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Strain number NIES-541  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Volvox aureus Ehrenberg var. aureus  
Synonym  
Former name  
Common name Green alga ; Volvocine algae  
Locality (Date of collection) Lake Yamanaka Yamanashi Japan (1981-**-**)  
Latitude / Longitude 35.4188881307 / 138.874219607 
Habitat (Isolation source) Freshwater (Sediment)  
History < Nozaki, Hisayoshi  
Isolator (Date of isolation) Nozaki, Hisayoshi (1981-07-06)  
Identified by Nozaki, Hisayoshi  
State of strain Subculture; Unialgal; Clonal; Axenic[2014 Jan]  
Culture condition
(Preculture condition)
Medium:  VT  
Temperature:  23 C
Light intensity:  28-32 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  42 D  
Gene information 18S rRNA ( LC086362 ) , atpB ( AB013998 ) , psaA ( AB044182 ) , psaB ( AB044424 ) , psbC ( AB044474 ) , rbcL ( D63445 )  
Cell size (min - max) - 3 μm  
Organization Colonial; Flagellate 
Characteristics Phototaxis  
Other strain no. Other strain no. : 1706-2  
Remarks Fragile species to transportation stresses; Read and agree "How to order 4.1" 
Movie https://www.youtube.com/watch?v=D0DKVeADVUs  
Reference
Nakada, T., Ito, T., Tomita, M. 2016 18S ribosomal RNA gene phylogeny of a colonial volvocalean lineage (Tetrabanaceae-Goniaceae-Volvocaceae, Volvocales, Chlorophyceae) and its close relatives. J. Jpn. Bot., 91, 345-354.
Keywords: 18S rRNA; colonial; green algae; molecular phylogeny; unicellular; Volvocales
Strain(s): 418456460474541544545564566571654666721725728735737867877879880881882883136222422246226625673388 

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19212223242526272829303132333435363738394041444546505152535455565758596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123124125127128129130131132133134135137138139144147148150151152153154155156157158159160162163164165166167168169170171172173174175176177178179180181182183185186187188189191192193194195197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268271287288289290294295297298299300301302303305306308309310311312313329334336337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

Nozaki, H., Misawa, K., Kajita, T., Kato, M., Nohara, S., Watanabe, M. M. 2000 Origin and evolution of the colonial Volvocales (Chlorophyceae) as inferred from multiple, chloroplast gene sequences. Mol. Phylogenet. Evol., 17, 256-268.
Strain(s): 418456460474541545564566569571572574577582653654666720721722724725727728730731732734735736737851854872882884886887888889890893895896898 

Nozaki, H., Ohta, N., Takano, H., Watanabe, M. M. 1999 Reexamination of phylogenetic relationships within the colonial Volvocales (Chlorophyta): an analysis of atpB and rbcL gene sequences. J. Phycol., 35, 104-112.
Keywords: atpB gene; Chlorophyta; Gonium; molecular phylogeny; rbcL gene; Volvocaceae; volvocine series; Volvox
Strain(s): 418456460541564566568569571572574577582653654666720721722724725727728730731732734735736737851852858861882884895 

Nozaki, H., Ito, M., Sano, R., Uchida, H., Watanabe, M. M., Kuroiwa, T. 1995 Phylogenetic relationships within the colonial Volvocales (Chlorophyta) inferred from rbcL gene sequence data. J. Phycol., 31, 970-979.
Keywords: Chlorophyta; Goniaceae; large subunit of ribulose-1,5-bisphosphate carboxylase / oxygenase (rbcL) gene; molecular phylogeny; Tetrabaenaceae; Volvocaceae
Strain(s): 418421456460541564566569571572574577653725731732734735737895 

Nozaki, H. 1994 Unequal flagellar formation in Volvox (Volvocaceae, Chlorophyta). Phycologia, 33, 58-61.
Strain(s): 541732 

Nozaki, H., Ito, M. 1994 Phylogenetic relationships within the colonial Volvocales (Chlorophyta) inferred from cladistic analysis based on morphological data. J. Phycol., 30, 353-365.
Keywords: Chlorophyta; cladistic analysis; Goniaceae; morphological data; phylogeny; sexual reproduction; Tetrabaena; Tetrabaenaceae fam. nov.; Tetrabaena socialis comb. nov.; Volvocaceae
Strain(s): 456460541565571654 

Nozaki, H. 1984 Volvox aureus Ehrenberg var. aureus. In Photomicrog. Fresh-w. Alg. [Tansui Sôrui Syashinsyû] Vol. 1, Eds. by Yamagishi, T. & Akiyama, M., Uchida Rokakuho, Tokyo, p. 97 (in Japanese & English).
Strain(s): 541542 

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