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Strain number NIES-415  
Phylum Chlorophyta  
Class Trebouxiophyceae  
Scientific name Actinastrum hantzschii Lagerheim  
Synonym -----  
Former name  
Common name Green alga  
Locality (Date of collection) Lake Kasumigaura Ibaraki Japan (1983-07-22)  
Latitude / Longitude 36.0428477398 / 140.373233439 
Habitat (Isolation source) Freshwater (Lake water)  
History < Kasai, Fumie  
Isolator (Date of isolation) Kasai, Fumie (1983-07-22)  
Identified by Watanabe, Masayuki  
State of strain Cryopreservation; Unialgal; Clonal; Non-axenic  
Culture condition
(Preculture condition)
Medium:  C (agar)  
Temperature:  20 (25) C
Light intensity:  4-10 (15-19) µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  3 M (14 D)  
Gene information 18S rRNA ( LC192144 )  
Cell size (min - max) - 17 μm  
Organization  
Characteristics  
Other strain no. Other strain no. : F7-4  
Remarks Cryopreserved 
Movie  
Reference
Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19212223242526272829303132333435363738394041444546505152535455565758596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123124125127128129130131132133134135137138139144147148150151152153154155156157158159160162163164165166167168169170171172173174175176177178179180181182183185186187188189191192193194195197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268271287288289290294295297298299300301302303305306308309310311312313329334336337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

Hayashi-Ishimaru, Y., Ohama, T., Kawatsu, Y., Nakamura, K., Osawa, S. 1996 UAG is a sense codon in several chlorophycean mitochondria. Curr. Genet., 30, 29-33.
Keywords: deviant genetic code; COXI; Chlorophyceaen mitochondria; release factor
Strain(s): 96209227415 

Watanabe, M. M., Kasai, F., Hiwatari, T., Suda, S., Nei, T. 1984 Cryopreservation of microalgae in liquid nitrogen - Survival of algal cultures after freezing. Jpn. J. Freez. Dry., 30, 23-26 (in Japanese with English title).
Strain(s): 1819212426293139404144454650737475767780818788899091939598991001011201221311351371441512042072132572633794152219223522392579 

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