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Strain number NIES-228  
Phylum Streptophyta  
Class Charophyceae  
Scientific name Closterium ehrenbergii Meneghini ex Ralfs  
Synonym -----  
Former name  
Common name Desmid  
Locality (Date of collection) Ebina Kanagawa Japan (1975-12-04)  
Latitude / Longitude 35.437621 / 139.393061 
Habitat (Isolation source) Freshwater (Paddy soil)  
History < Ichimura, Terunobu  
Isolator (Date of isolation) Ichimura, Terunobu (-----)  
Identified by Ichimura, Terunobu  
State of strain Subculture; Unialgal; Clonal; Axenic[2013 Jan]  
Culture condition
(Preculture condition)
Medium:  C  
Temperature:  20 (25) C
Light intensity:  4-8 (12-15) µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  3 M (1 M)  
Gene information 18S rRNA ( AY148818 ) , 5.8S-ITS2-28S ( AY148895 ) , cdip-1 mRNA ( AB066448 ) , CeRLK1 ( AB306552 ) , CeRLK2 ( AB306553 ) , CeRLK3 ( AB306554 ) , CeRLK4 ( AB306555 ) , CeRLK5 ( AB306556 ) , CeRLK6 ( AB306557 ) , CeRLK7 ( AB306558 ) , CeRLK8 ( AB306559 ) , CeRLK9 ( AB306560 ) , CeRLK10 ( AB306561 ) , CeRLK11 ( AB306562 ) , CeRLK12 ( AB306563 ) , CeRLK13 ( AB306564 ) , CeRLK14 ( AB306565 )  
Cell size (min - max) 80 - 500 μm  
Organization Unicellular 
Characteristics Heterothallic ; Mating type (+) ; Mating group B ; Crosses with NIES-229  
Other strain no. Other strain no. : KK-33-1  
Shinozaki, A., Sato, N., Hayashi, Y. 2009 Peroxisomal targeting signals in green algae. Protoplasma, 235, 57-66.
Keywords: Closterium; green algae; peroxisomal targeting signal (PTS); peroxisome; transport
Strain(s): 228 

Sasaki, G., Katoh, K., Hirose, N., Suga, H., Kuma, K., Miyata, T., Su, Z. H. 2007 Multiple receptor-like kinase cDNAs from liverwort Marchantia polymorpha and two charophycean green algae, Closterium ehrenbergii and Nitella axillaris: Extensive gene duplications and gene shufflings in the early evolution of streptophytes. Gene, 401(1-2), 135-144.
Keywords: conjugate alga; stonewort; gene family; divergence; phylogenetic tree; molecular evolution
Strain(s): 228 

Ciniglia, C., Cascone, C., Giudice, R. L., Pinto, G., Pollio, A. 2005 Application of methods for assessing the geno- and cytotoxicity of Triclosan to C. ehrenbergii. J. Hazard. Mater., 122, 227-232.
Keywords: Closterium ehrenbergii; Triclosan; sexual reproduction; vegetative reproduction; comet assay
Strain(s): 228229 

Fukumoto, R., Fujii, T., Sekimoto, H. 2003 Cloning of a cDNA encoding a sexual cell division-inducing pheromone from a unicellular green alga Closterium ehrenbergii. J. Phycol., 39, 931-936.
Keywords: Closterium; conjugation; pheromone; sexual reproduction; RACE-PCR
Strain(s): 228229 

Denboh, T., Ichimura, T., Hendrayanti, D., Coleman, A. W. 2003 Closterium moniliferum-ehrenbergii (Charophyceae, Chlorophyta) species complex viewed from the 1506 Group I intron and ITS2 of nuclear rDNA. J. Phycol., 39, 960-977.
Keywords: biological species; Closterium; group I introns; ITS2; mating group; molecular phylogeny; speciation
Strain(s): 172173174182183228229 

Fukumoto, R., Dohmae, N., Takio, K., Satoh, S., Fujii, T., Sekimoto, H. 2002 Purification and characterization of a pheromone that induces sexual cell division in the unicellular green alga Closterium ehrenbergii. Plant Physiol. Biochem., 40, 183-188.
Keywords: Closterium ehrenbergii; pheromone; sexual reproduction
Strain(s): 228229 

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 1922232425262728293031323334353637383944454650515253545556576061626364656667707475787980818789909192939495979899100102105106107108109110111112119120123124127128129130131132133134135137138139144147148150151152153154155156157158159160162163164166167168169170171172173174175176177178179180181182183185186187188189191192193194195197198199200201202203205206207208209210211212213214215216217224226227228229230231232241242243244246248259262263266267268271287288289290294295297298299300301302303305306308309310311312313329336337338339340341342349351359360361362375378379380382384385390394396397415418419422423424426427429430431432433434436437438447448449450451452453457458459464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540542543544545546564565566567568570572573574575576577579580581584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653655656657658659660661662663664665666667672685686687688689690692693694718719720723724726727728729 

Imamura, N., Motoike, I., Shimada, N., Nishikori, M., Morisaki, H., Fukami, H. 2001 An efficient screening approach for anti-Microcystis compounds based on knowledge of aquatic microbial ecosystem. J. Antibiotics, 54, 582-587.
Strain(s): 102228 

Ogawa, S., Hirota, K. 2000 Disintegration of chloroplasts during zygote maturation in Closterium ehrenbergii (Zygnematales, Chlorophyta). Int. J. Plant Sci., 161, 609-614.
Keywords: chloroplast; Closterium; conjugation; disintegration of chloroplast; Zygnematales; zygote maturation
Strain(s): 228229 

Sekimoto, H. 2000 Intercellular communication during sexual reproduction of Closterium (Conjugatophyceae). J. Plant Res., 113, 343-352.
Keywords: chemotaxis; Closterium; conjugation; mating; sex pheromone; sexual reproduction
Strain(s): 6465228229 

Fukumoto, R., Fujii, T., Sekimoto, H. 1998 A newly identified chemotactic sexual pheromone from Closterium ehrenbergii. Sex. Plant Reprod., 11, 81-85.
Keywords: Closterium ehrenbergii; chemoattractant; conjugation; sexual pheromone; protoplast-release inducing protein
Strain(s): 228229 

Fukumoto, R., Fujii, T., Sekimoto, H. 1997 Detection and evaluation of a novel sexual pheromone that induces sexual cell division of Closterium ehrenbergii (Chlorophyta). J. Phycol., 33, 441-445.
Keywords: Chlorophyta; Closterium ehrenbergii; conjugation; gametogenesis; gamete; pheromone; protoplast-release-inducing protein; sexual cell division
Strain(s): 228229 

Kim, Y.-S., Oyaizu, H., Matsumoto, S, Watanabe, M. M., Nozaki, H. 1994 Chloroplast small-subunit ribosomal RNA gene sequence from Chlamydomonas parkeae (Chlorophyta) : molecular phylogeny of a green alga with a peculiar pigment composition. Eur. J. Phycol., 29, 213-217.
Keywords: Chlamydomonas parkeae; Chlorophyta; chloroplast small-subunit (SSU) rRNA; phylogeny; pigment composition
Strain(s): 228254468666 

Hino, S. 1991 An examination of algal bioassay method for agricultural chemicals and heavy metals in a water environment. Hokkaido Kankyô Kagaku Kenkyû Sentâ Syohô, 18, 41-49 (in Japanese with English summary).
Strain(s): 228229 

Kasai, F., Ichimura, T. 1990 Temperature optima of three closely related mating groups of the Closterium ehrenbergii (Chlorophyta) species complex. Phycologia, 29, 396-402.
Strain(s): 228229 

Ichimura, T., Kasai, F. 1987 Time-lapse analyses of sexual isolation between two closely related mating groups of the Closterium ehrenbergii species complex (Chlorophyta). J. Phycol., 23, 523-534.
Keywords: Closterium ehrenbergii; desmids; mating group; multiple-choice mating; no-choice mating; sexual interactions; sexual isolation; time-lapse photomicrography
Strain(s): 228229 

Kasai, F., Ichimura, T. 1986 Morphological variabilities of three closely related mating groups of Closterium ehrenbergii Meneghini (Chlorophyta). J. Phycol., 22, 158-168.
Keywords: cell shape and size; clonal culture; Closterium ehrenbergii; mating group; morphological variability; statistical analysis
Strain(s): 171228229 

Ichimura, T., Kasai, F. 1984 Time lapse analyses of sexual reproduction in Closterium ehrenbergii (Conjugatophyceae). J. Phycol., 20, 258-265.
Keywords: Closterium ehrenbergii; conjugation; desmids; mating type; sexual reproduction
Strain(s): 228229 

Ichimura, T. 1983 Hybrid inviability and predominant survival of mating type minus progeny in laboratory crosses between two closely related mating groups of Closterium ehrenbergii. Evolution, 37, 252-260.
Strain(s): 228229 

Ichimura, T. 1981 Mating types and reproductive isolation in Closterium ehrenbergii Meneghini. Bot. Mag. Tokyo, 94, 325-334.
Keywords: biosystematic study; Closterium ehrenbergii; mating group; mating type; reproductive isolation
Strain(s): 228229 
DOI: 10.1007/BF02493393

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