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Strain number NIES-57  
Phylum Streptophyta  
Class Charophyceae  
Scientific name Closterium peracerosum-strigosum-littorale complex  
Synonym -----  
Former name  
Common name Desmid  
Locality (Date of collection) Katsuta Ibaraki Japan (1975-05-**)  
Latitude / Longitude  
Habitat (Isolation source) Freshwater (Paddy water)  
History < Watanabe, Makoto M.  
Isolator (Date of isolation) Watanabe, Makoto M. (1975-05-**)  
Identified by Watanabe, Makoto M.  
State of strain Subculture; Unialgal; Clonal; Axenic[2014 Jan]  
Culture condition
(Preculture condition)
Medium:  C  
Temperature:  15 (20) C
Light intensity:  7-14 (10-17) µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  3 M (21 D)  
Gene information  
Cell size (min - max)  
Organization Unicellular 
Characteristics Heterothallic: Mating type (+) ; Mating group II A  
Other strain no. Other strain no. : IB-8-25  
Remarks  
Movie  
Reference
Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19212223242526272829303132333435363738394041444546505152535455565758596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123124125127128129130131132133134135137138139144147148150151152153154155156157158159160162163164165166167168169170171172173174175176177178179180181182183185186187188189191192193194195197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268271287288289290294295297298299300301302303305306308309310311312313329334336337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

Otsuki, A., Watanabe, M. M., Sugahara, K. 1987 Chlorophyll pigments in methanol extracts from ten axenic cultured diatoms and three green algae as determined by reverse phase HPLC with fluorometric detection. J. Phycol., 23, 406-414.
Keywords: chlorophyll c complex; chlorophyll pigments; diatoms; field desorption mass spectrometry; methanol extracts; reverse phase HPLC
Strain(s): 16565771157225534 

Watanabe, M. M., Ichimura, T. 1978 Biosystematic studies of the Closterium peracerosum-strigosum-littorale complex. II. Reproductive isolation and morphological variation among several populations from the Northern Kanto area in Japan. Bot. Mag. Tokyo, 91, 1-10.
Strain(s): 5152535455565758596061626364656970261 

Watanabe, M. M., Ichimura, T. 1978 Biosystematic studies of the Closterium peracerosum-strigosum-littorale complex. III. Degrees of sexual isolation among the three population groups from the Northern Kanto area. Bot. Mag. Tokyo, 91, 11-24.
Strain(s): 5152535455565758596061626364656970261 

Watanabe, M. M. 1977 Biosystematics in Closterium of sexual unicellular green algae and Calothrix and Spirulina of asexual filamentous blue-green algae, with special reference to the analyses of natural populations. Thesis D. Sci., Hokkaido University, 114 pp., 28 tables, 54 figs.
Strain(s): 3945465152535455565758596061626364656667686970261262266268 

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