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Strain number NIES-1335  
Phylum Kathablepharida  
Class Kathablepharidea  
Scientific name Leucocryptos marina (Braarud) Butcher  
Synonym  
Former name  
Common name  
Locality (Date of collection) Tokyo Bay Japan (2003-10-16)  
Latitude / Longitude  
Habitat (Isolation source) Marine (Seawater)  
History < TKB;  
Isolator (Date of isolation) Okamoto, Noriko (2003-12-**)  
Identified by Okamoto, Noriko  
State of strain Subculture; Mixed (Chrysochromulina sp. (NIES-1333) as food); Non-clonal; Non-axenic  
Culture condition
(Preculture condition)
Medium:  mIMR  
Temperature:  20 C
Light intensity:  4-5 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information 18S rRNA ( AB194980 DQ980480 DQ980481 ) , LSU rRNA ( DQ980471 ) , actin ( DQ980424 DQ980425 DQ980426 DQ980439 DQ980440 DQ980441 ) , alpha-tubulin ( DQ980496 DQ980535 DQ980537 DQ980538 ) , beta-tubulin ( AB194977 AB194978 DQ980511 FJ973420 ) , eEF-2 ( EU812190 EU812191 EU812192 EU812193 EU812194 EU812195 ) , Hsp70 ( EU812178 EU812179 EU812180 EU812181 EU812182 ) , Hsp90 ( DQ980461 DQ980462 DQ980463 ) , nad11 ( AB693966 )  
Cell size (min - max) - 15 μm  
Organization Unicellular; Flagellate 
Characteristics Heterotrophic ; Phagotrophic  
Other strain no. Other strain no. : TKB-081 (nrc057)  
Remarks  
Movie  
Reference
Nishimura, Y., Kamikawa, R., Hashimoto, T., Inagaki, Y. 2012 Separate origins of group I introns in two mitochondrial genes of the katablepharid Leucocryptos marina. PLoS One, 7, e37307 (article ID).
Strain(s): 13331335 
DOI: 10.1371/journal.pone.0037307

Reeb, V. C., Peglar, M. T., Yoon, H. S., Bai, J. R., Wu, M., Siu, P., Grafenberg, J. L., Reyes-Prieto, A., Rummele, S. E., Gross, J., Bhattacharya, D. 2009 Interrelationships of chromalveolates within a broadly sampled tree of photosynthetic protists. Mol. Phylogenet. Evol., 53, 202–211.
Keywords: Chromalveolata; Multigene phylogenetics; Plastidophila; Rhizaria; Supergroup; Tree of life
Strain(s): 13341335 
DOI: 10.1016/j.ympev.2009.04.012

Kim, E., Graham, L. E. 2008 EEF2 analysis challenges the monophyly of Archaeplastida and Chromalveolata. PLoS One, 3, e0002621 (article ID).
Strain(s): 1335 
DOI: 10.1371/journal.pone.0002621

Kim, E., Simpson, A. G., Graham, L. E. 2006 Evolutionary relationships of apusomonads inferred from taxon-rich analyses of nuclear encoded genes. Mol. Biol. Evol., 23, 2455-2466.
Keywords: Apusomonas; Apusomonadidae; Opisthokonta; Amoebozoa; protists; evolution
Strain(s): 93695513331335 
DOI: 10.1093/molbev/msl120

Okamoto, N., Inouye, I. 2005 The katablepharids are a distant sister group of the Cryptophyta: a proposal for Katablepharidophyta divisio nova/Kathablepharida phylum novum based on SSU rDNA and beta-tubulin phylogeny. Protist, 156, 163-179.
Keywords: beta-tubulin; Cryptophyta; Katablepharidophyta/Kathablepharida; Katablepharis/Kathablepharis japonica; Leucocrypta; Leucocryptos marina; phylogeny; SSU rDNA
Strain(s): 133313341335 

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