Strain number NIES-109  
Phylum Cyanophyta  
Class Cyanophyceae  
Scientific name Microcystis aeruginosa (Kützing) Lemmermann  
Synonym Microcystis ichthyoblabe Kützing; Microcystis novacekii (Komárek) Compère; Microcystis viridis (A.Brown) Lemmermann; Microcystis wesenbergii Komárek  
Former name Microcystis wesenbergii Komárek  
Common name Blue-green alga ; Cyanobacteria  
Locality (Date of collection) Lake Yogo, Shiga, Japan (1982-07-**)  
Latitude / Longitude 35.5231422558 / 136.194054928 
Habitat (Isolation source) Freshwater (Lake water)  
History < Watanabe, Makoto M.  
Isolator (Date of isolation) Watanabe, Michiko H. (1982-07-**)  
Identified by Watanabe, Michiko H.  
State of strain Cryopreservation; Unialgal; Clonal; Non-axenic  
Culture condition
(Preculture condition)
Medium:  MA  
Temperature:  25 C
Light intensity:  20-30 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information 16S rRNA ( AB023270 ) , ftsZ ( AB324864 ) , glnA ( AB324943 ) , gltX ( AB325022 ) , gyrB ( AB325101 ) , pgi ( AB325180 ) , recA ( AB325259 ) , tpi ( AB325338 )  
Cell size (min - max)  
Organization  
Characteristics Cyanobacterial water bloom (aoko) ; ST15 (Tanabe et al. 2007)  
Other strain no. Other strain no. : Y-MW-24  
Remarks Cryopreserved 
Movie  
Reference
Yamada, E., Sasai, K., Higa, R., Mizuguchi, H., Fuse Y. 2020 Characterization and dynamic analysis of dissolved proteins in Lake Biwa using two-dimensional electrophoresis. Anal. Sci., 36, 753-759.
Strain(s): 109275391665 
PubMed: 31956160
DOI: 10.2116/analsci.19P450

Tanabe, Y., Hodoki, Y., Sano, T., Tada, K., Watanabe, MM. 2018a Adaptation of the freshwater bloom-forming cyanobacterium Microcystis aeruginosa to brackish water is driven by recent horizontal transfer of sucrose genes. Front. Microbiol., 9, 1150 (article ID).
Keywords: Microcystis; bloom; salt tolerance; sucrose; genomics; brackish water; horizontal gene trasnfer; ecotype
Strain(s): 4487888990919899100101102103104105107108109112298299478604843901105010521059106310671069107010751076108510931094109811001101110211041105110611071108111311141115112211281130113111321134113911411142114311441150117011741175117611771179118111831185121112131214121512321234123812391252208820902091246524662467246824692470247124722473247524762477247824792480248124822483248424852486248724882489249024912492249424952546254725482549255025512552255325542555255625572558255925602561259225942595259625972598259926002601260226032604260526062607260826102611261226132617261826192620262126222623262426252626262826294234 
PubMed: 29922255
DOI: 10.3389/fmicb.2018.01150

Yamada, E., Ueda, T., Tanaka, T., Fujii, K., Mizuguchi, H., Fuse, Y. 2018 Effects of photoirradiation on the characteristics of dissolved organic matter in Lake Biwa and its surrounding rivers. J. Environ. Safety, 9, 23-32.
Keywords: photoirradiation; wavelength cut filter; photodegradation; dissolved organic matter; fulvic-like fluorophore; protein-like fluorophore; environmental waters
Strain(s): 109275665 
DOI: 10.11162/daikankyo.E17RP1101

Mizuguchi, H., Fujii, S., Fujii, S., Higa, R., Ishikawa, K., Hayakawa, K., Okamoto, T., Fuse, Y., Karatani, H., Yamada, E. 2018 Application of SYBR green real-time PCR assay to monitoring of phytoplankton during cultivation and in Lake Biwa. Limnological Study, , 3-12.
Keywords: SYBR Green; real-time PCR; phytoplankton; Lake Biwa; dissolved organic matter
Strain(s): 109275391665 

Sun, L.W., Jiang, W.J., Sato, H., Kawachi, M., Lu, X.W. 2016 Rapid classification and identification of Microcystis aeruginosa strains using MALDI-TOF MS and polygenetic analysis. PLoS One, 11, e0156275 (article ID).
Strain(s): 44878889901001011041051071091111124786048439011043105310581061106810691070107210761077108510941099110111041105111311151126113411351144115111571170121112131215121612171218122212241230123212331239 
PubMed: 27227555
DOI: 10.1371/journal.pone.0156275

Yamada, E., Hirota, T., Hatori, N., Kitao, Y., Fuse, Y., Aoki, S., Karatani, H., Matsunaga, T. 2012 Characterization of protein-like fluorophores released from lake phytoplankton on the basis of fractionation and electrophoresis. Anal. Sci., 28, 595-600.
Strain(s): 109275665 
PubMed: 22729046
DOI: 10.2116/analsci.28.595

Yamada, E., Ohara, S., Uehara, T., Hirota, T., Hatori, N., Fuse, Y., Aoki, S. 2012 Biodegradation of dissolved organic matter (DOM) released from phytoplankton in Lake Biwa. Anal. Sci., 28, 675-681.
Strain(s): 109275665 
PubMed: 22790369
DOI: 10.2116/analsci.28.675

Tanabe, Y. & Watanabe, M. M. 2011 Local expansion of a panmictic lineage of water bloom-forming cyanobacterium Microcystis aeruginosa. PLoS One, 6, e17085 (article ID).
Strain(s): 4487888990919899100101102103104105106107108109110111112298299478604843901105010511052105310541055105610571058105910601061106210631064106510661067106810691070107210751076107710851090109110921093109410951096109710981100110111021103110411051106110711081113111411151122112311241125112611271128112911301131113211331134113511361137113811391140114111421143114411451146114711481149115011511152115311551156115711591160116111621163116411701171117211731174117511761177117911801181118311841185118612091211121312141215121612171218121912201221122212231224122512261227122812291230123112321233123412351236123712381239124012411242124312441245125112521253125420882089209020912465246624672468246924702471247224732474247524762477247824792480248124822483248424852486248724882489249024912492249324942495249625422543254425452546254725482549255025512552255325542555255625572558255925602561259125922593259425952596259725982599260026012602260326042605260626072608260926102611261226132617261826192620262126222623262426252626262726282629 
PubMed: 21390221
DOI: 10.1371/journal.pone.0017085

Tanabe, Y., Sano, T., Kasai, F., Watanabe, M. M. 2009b Recombination, cryptic clades and neutral molecular divergence of the microcystin synthetase (mcy) genes of toxic cyanobacterium Microcystis aeruginosa. BMC Evol. Biol., 9, 115 (article ID).
Strain(s): 44878889909198991001011021031041051061071081091101111122982994786048439011050105110521053105410551056105710581059106010611062106310641065106610671068106910701072107510761077108510901091109210931094109510961097109811001101110211031104110511061107110811131114111511221123112411251126112711281129113011311132113311341135113611371138113911401141114211431144114511461147114811491150115111521153115511561157115911601161116211631164117011711172120912111213121412151216121712181219122012211222122312241225122612271228122912301231123212331234123512361237123812391240124112421243124412451251125212531254246524662467246924702471247224732474247524762477247824792561 
PubMed: 19463155
DOI: 10.1186/1471-2148-9-115

Ohara, S., Uehara, T., Kimura, K. Yoshida, T., Fujiwara, S., Mizuguchi, H., Fuse, Y., Yamada, E. 2009 Behavior of humic substances and algal dissolved organic matter as precursors of trihalomethane in Lake Biwa. Bunseki Kagaku, 58, 231-240 (in Japanese with English summary).
Keywords: dissolved organic matter (DOM); humic substances; algal DOM; trihalomethane precursors; Lake Biwa
Strain(s): 109275665 
DOI: 10.2116/bunsekikagaku.58.231

Aoki, S., Ohara, S., Kimura, K., Mizuguchi, H., Fuse, Y., Yamada, E. 2008 Characterization of dissolved organic matter released from Microcystis aeruginosa. Anal. Sci., 24, 389-394.
Strain(s): 109 
PubMed: 18332548
DOI: 10.2116/analsci.24.389

Aoki, S., Ohara, S., Kimura, K., Mizuguchi, H., Fuse, Y., Yamada, E. 2008 Characterization of fluorophores released from three kinds of lake phytoplankton using gel chromatography and fluorescence spectrophotometry. Anal. Sci., 24, 1461-1467.
Strain(s): 109275665 
PubMed: 18997376
DOI: 10.2116/analsci.24.1461

Tanabe, Y., Kasai, F., Watanabe, M. M. 2007 Multilocus sequence typing (MLST) reveals high genetic diversity and clonal population structure of the toxic cyanobacterium Microcystis aeruginosa. Microbiology, 153, 3695-3703.
Strain(s): 44878889909198991001011021031041051061071081091101111122982994786048439019331050105110521053105410551056105710581059106010611062106310641065106610671068106910701072107510761077108510901091109210931094109510961097109811001101110211031104110511061107110811131114111511221123112411251126112711281129113011311132113311341135113611371138113911401141114211431144114511461147114811491150115111521153115511561157115911601161116211631164117011711172120912111213121412151216121712181219122012211222122312241225122612271228122912301231123212331234123512361237123812391240124112421243124412451251125212531254 
PubMed: 17975077
DOI: 10.1099/mic.0.2007/010645-0

Yoshida, T., Yuki, Y., Lei, S., Chinen, H., Yoshida, M., Kondo, R., Hiroishi, S. 2003 Quantitative detection of toxic strains of the cyanobacterial genus Microcystis by competitive PCR. Microbes Environ., 18, 16-23.
Keywords: competitive PCR; microcystin; Microcystis; peptide synthetase; toxic cyanobacteria
Strain(s): 87888990102104105106108109110111112298299604 
DOI: 10.1264/jsme2.18.16

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 192122232425262728293031323334353637383940414445465051525354555658596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123125127128129130131132133134135137138139144147151152153154155156157158159160162163164165166167168170171172173174175176177178179180181182183185186187188189191192193194197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268287288289290294295297298299300301302303305306308309310312313329334337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

Yuki, Y., Yoshida, T., Hiroishi, S. 2002 Molecular detection of the toxic cyanobacteria [Yûdoku aoko no bunshi-shikibetsu to yosatsu he-no ôyô]. In Series of Fisheries Science Vol. 143, Prevention and Extermination Strategies of Harmful Algal Blooms [Suisan-gaku shirîzu 134, Yûgai-yûdoku sôrui burûmu no yobô to kujo] , Eds. by Hiroishi, S., Imai, I. & Ishimaru, T., Koseisha-Koseikaku, Tokyo, pp. 43-53 (in Japanese).
Strain(s): 738187888990919899100101102104105106108109110111112204230298299604 

Kondo, R., Kagiya, G., Hiroishi, S., Watanabe, M. 2000 Genetic typing of a bloom-forming cyanobacterial genus Microcystis in Japan using 16S rRNA gene sequence analysis. Plankton Biol. Ecol., 47, 1-6.
Keywords: genetic typing; 16S rDNA; Microcystis; cyanobacteria; phylogenetic analysis
Strain(s): 878889909198991001011021041051061081091101111122982996041055106110681076109010911105112211421164 

Kondo, R., Kagiya, G., Komura, M., Yuki, Y., Hiroishi, S., Watanabe, M. 2000 Molecular taxonomy of a bloom-forming cyanobacterial genus Microcystis. Bull. Plankton Soc. Japan, 47, 25-33 (in Japanese with English summary).
Keywords: cyanobacteria; Microcystis; bacterial criteria; molecular analysis
Strain(s): 6878889909198991001011021041051061081091101111122982996041055106110681076109010911105112211421164 

Kondo, R., Kagiya, G., Hiroishi, S., Watanabe, M., Hata, Y. 1999 Rapid DNA extraction from a bloom-forming cyanobacterium. Microbes Environ., 14, 157-161.
Keywords: DNA extraction; PCR; Microcystis; cyanobacteria
Strain(s): 878889909198991001011021041051061081091101111122982996041055106110681076109010911105112211421164 
DOI: 10.1264/jsme2.14.157

Kondo, R., Kagiya, G., Yuki, Y., Hiroishi, S., Watanabe, M. 1998 Taxonomy of a bloom-forming cyanobacterial genus Microcystis. Nippon Suisan Gakkaishi, 64, 291-292 (in Japanese with English title).
Strain(s): 87888990919899100101102104105106107108109110111112298299604 

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