Strain number NIES-304  
Phylum Dinophyta  
Class Dinophyceae  
Scientific name Peridinium willei Huitfeldt-Kaas  
Synonym  
Former name  
Common name Dinoflagellate  
Locality (Date of collection) Tsukiyono Gunma Japan (1984-06-01)  
Latitude / Longitude  
Habitat (Isolation source) Freshwater (Pond water)  
History < Sawaguchi, Tomohiro  
Isolator (Date of isolation) Sawaguchi, Tomohiro (1984-06-14)  
Identified by Sawaguchi, Tomohiro  
State of strain Subculture; Unialgal; Clonal; Axenic[2017 Nov]  
Culture condition
(Preculture condition)
Medium:  Carefoot  
Temperature:  15 C
Light intensity:  20 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  2 M  
Gene information 18S rRNA ( AF274272 ) , act ( AF482420 ) , beta-tubulin ( AF482421 )  
Cell size (min - max) - 50 μm  
Organization Unicellular; Flagellate 
Characteristics Homothallic  
Other strain no. Other strain no. : 8423-P  
Remarks Fragile species to transportation stresses; Read and agree "How to order 4.1"; Axenic 
Movie  
Reference
Logares, R., Shalchian-Tabrizi, K., Boltovskoy, A., Rengefors, K. 2007 Extensive dinoflagellate phylogenies indicate infrequent marine–freshwater transitions. Mol. Phylogenet. Evol., 45, 887-903.
Keywords: Dinoflagellates; Freshwater; Colonization; Phylogeny
Strain(s): 304463471495 
PubMed: 17928239
DOI: 10.1016/j.ympev.2007.08.005

Hamana, K., Sakamoto, A., Nishina, M., Niitsu, M. 2004 Cellular polyamine profile of the phyla Dinophyta, Apicomplexa, Ciliophora, Euglenozoa, Cercozoa and Heterokonta. J. Gen. Appl. Microbiol., 50, 297-303.
Keywords: Apicomlexa; Cercozoa; Ciliophora; Dinophyta; Euglenozoa; Heterokonta; norspermidine; polyamine
Strain(s): 12286304331381387463 
PubMed: 15747233
DOI: 10.2323/jgam.50.297

Saldarriaga, J. F., McEwan, M. L., Fast, N. M., Taylor, F. J. R., Keeling, P. J. 2003 Multiple protein phylogenies show that Oxyrrhis marina and Perkinsus marinus are early branches of the dinoflagellate lineage. Int. J. Syst. Evol. Microbiol., 53, 355-365.
Strain(s): 304 
PubMed: 12656195
DOI: 10.1099/ijs.0.02328-0

Saldarriaga, J. F., Taylor, F. J. R., Keeling, P. J., Cavalier-Smith, T. 2001 Dinoflagellate nuclear SSU rRNA phylogeny suggests multiple plastid losses and replacements. J. Mol. Evol., 53, 204-213.
Keywords: Plastid; Dinoflagellates; Small subunit rRNA; Phylogeny; Endosymbiosis
Strain(s): 304463 
PubMed: 11523007
DOI: 10.1007/s002390010210

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