A big image opens when the thumbnail is clicked.(nies-0223.jpg)
Strain number NIES-223  
Phylum Heterokontophyta  
Class Bacillariophyceae  
Scientific name Skeletonema marinoi-dohrnii complex  
Synonym  
Former name Skeletonema costatum (Greville) Cleve  
Common name Centric diatom  
Locality (Date of collection) Shodo Isl. Kagawa Japan (1979-07-12)  
Latitude / Longitude  
Habitat (Isolation source) Marine  
History < KAGAWA  
Isolator (Date of isolation) Yuki, Katsuhisa  
Identified by  
State of strain Subculture; Unialgal; Clonal; Non-axenic  
Culture condition
(Preculture condition)
Medium:  f/2  
Temperature:  10 C
Light intensity:  3-6 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information 18S rRNA ( AB488609 ) , 28S rRNA ( DQ396507 ) , CO1 ( AB706229 )  
Cell size (min - max) - 5 μm  
Organization Unicellular 
Characteristics Red tide ; Planktonic ; DNA sequencing indicates this strain is included in Skeletonema marinoi/dohrnii clade  
Other strain no. Other strain no. : KGW-26  
Remarks  
Movie  
Reference
Yamada, M., Otsubo, M., Tsutsumi, Y., Mizota, C., Nakamura, Y., Takahashi, K., Iwataki M. 2017 Utility of mitochondrial-encoded cytochrome c oxidase I gene for phylogenetic analysis and species identification of the planktonic diatom genus Skeletonema. Phycol. Res., 65, 217-225.
Keywords: cox1; genetic marker; LSU rDNA; phylogenetictree; species delimitation; SSU rDNA
Strain(s): 16223283728382842 
DOI: 10.1111/pre.12179

Mitani, E., Nakayama, F., Matsuwaki, I., Ichi, I., Kawabata, A., Kawachi, M., Kato, M. 2017 Fatty acid composition profiles of 235 strains of three microalgal divisions within the NIES Microbial Culture Collection. Microb. Resour. Syst., 33, 19-29.
Keywords: Cryptophyta; docosahexaenoic acid; eicosapentaenoic acid; fatty acid; Haptophyta; Heterokontophyta; microalgae
Strain(s): 189141517711151452232252332342652742752762772782792802812822842933233243303333443453463473483503533723773883913954074084094134144174614624664875345485535565575585595605625875885895906036056216226236956966976986997007017027037047057067077087097107117127137147157167417657667678058379971001100210031004100510061007100910111016101710441045104610471302130313241327133013391340134913531370137513761379138313841385138613871391139213931395139813991400140116991700173018131815181618261827183118621863186418651874196319641965214221432144214521472148230023312332235123632364236523692370237625062533253425352536253725902633266826892690269126932694269626972716271727182720272227232725272627292730273127322770277127722773283928402841284228432844285928722878289028993391 

Kooistra, W. H. C. F., Sarno, D., Balzano, S., Gu, H., Andersen, R. A., Zingone, A. 2008 Global diversity and biogeography of Skeletonema species (Bacillariophyta). Protist, 159, 177-193.
Keywords: biogeography; cryptic species; distribution; LSU rDNA; phytoplankton; Skeletonema costatum
Strain(s): 1617223323324 
PubMed: 18042429
DOI: 10.1016/j.protis.2007.09.004

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