Strain Data

Strain number		NIES-230
Phylum		Cyanophyta
Class		Cyanophyceae
Scientific name		Merismopedia tenuissima Lemmermann
Synonym
Former name
Common name		Blue-green alga ; Cyanobacteria
Locality (Date of collection)		Tsukuba, Ibaraki, Japan (1984-05-07)
Latitude / Longitude		36.105319 / 140.081738
Habitat (Isolation source)		Freshwater (Pond water)
History		< Kasai, Fumie
Isolator (Date of isolation)		Kasai, Fumie (1984-05-09)
Identified by		Watanabe, Makoto M.
State of strain		Cryopreservation; Unialgal; Clonal; Non-axenic
Culture condition (Preculture condition)		Medium: C Temperature: 20 C Light intensity: 15-22 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 1 M
Gene information
Cell size (min - max)		- 3 μm
Organization
Characteristics
Other strain no.		Other strain no. : F98-2
Remarks		Cryopreserved
Movie

Reference
Asukabe, H., Akahori, S., Ueno, E., Nakayama, T., Yamashita, R., Arii, S., Harada, K., Imanishi, S. Y. 2020 Cyanobacterial classification with the toxicity using MALDI Biotyper. J. Am. Soc. Mass Spectrom., 31, 1572-1578. Keywords: cyanobacteria; MALDI Biotyper; microcystins; profiling Strain(s): 27, 42, 73, 81, 87, 89, 100, 101, 102, 103, 104, 105, 107, 111, 112, 207, 208, 230, 266, 298, 416, 433, 478, 510, 512, 592, 593, 604, 611, 808, 809, 835, 843, 846, 847, 905, 928, 931, 932, 981, 989, 1025, 1043, 1058, 1061, 1064, 1068, 1069, 1071, 1076, 1085, 1099, 1101, 1105, 1113, 1115, 1126, 1133, 1134, 1135, 1144, 1157, 1215, 1642, 1652, 1668, 1672, 1673, 1678, 1698, 2087, 2131, 2412, 3708, 3733, 3734, 3736, 3755, 3756, 3757 PubMed: 32501712 DOI: 10.1021/jasms.0c00148 Hodoki, Y., Ohbayashi, K., Kobayashi, Y., Okuda, N., Nakano, S. 2012 Detection and identiﬁcation of potentially toxic cyanobacteria: Ubiquitous distribution of Microcystis aeruginosa and Cuspidothrix issatschenkoi in Japanese lakes. Harmful Algae, 16, 49-57. Keywords: Anatoxin-a; Automated rRNA intergenic spacer analysis (ARISA); Cuspidothrix issatschenkoi; Cyanotoxin; Microcystin; Microcystis aeruginosa Strain(s): 40, 77, 111, 230, 512, 843, 905, 928, 1645, 1669, 1950 DOI: 10.1016/j.hal.2012.01.003 Ioki, M., Ohkoshi, M., Nakajima, N., Nakahira-Yanaka, Y., Watanabe, M. M. 2012 Isolation of herbicide-resistant mutants of Botryococcus braunii. Bioresour. Technol., 109, 300-303. Keywords: Botryococcus braunii; Paraquat; Methyl viologen; Basta; Glufosinate Strain(s): 35, 230 PubMed: 21906932 DOI: 10.1016/j.biortech.2011.07.101 Ishihara, S., Horio, T., Kobara, Y., Yokoyama, A. 2006 Evaluation of herbicide effects on microalgal cells by flow cytometric analysis. J. Weed Sci. Tech., 51, 239-248 (in Japanese with English summary). Keywords: herbicide; flow cytometry; algae; morphological change; recoverability; ecological risk assessment Strain(s): 230 DOI: 10.3719/weed.51.239 Kim, B.-H., Oh, H.-M., Lee, Y.-K., Choi, G.-G., Ahn, C.-y., Yoon, B.-D., Kim, H.-S., 2006 Simple method for RNA preparation from cyanobacteria. J. Phycol., 42, 1137-1141. Keywords: bead–phenol–chloroform method; cyanobacteria; RNA extraction; xanthogenate–SDS–phenol method Strain(s): 230 DOI: 10.1111/j.1529-8817.2006.00263.x Hirabayashi, R. & Imamura, N. 2003 Action mechanism of a selective anti-cyanobacterial compound, argimicin A. J. Antibiotics, 56, 154-159. Strain(s): 45, 81, 204, 230, 592 PubMed: 12715875 DOI: 10.7164/antibiotics.56.154 Yamaguchi, T., Kobayashi, Y., Adachi, K., Imamura, N. 2003 Argimicins B and C, new anti-cyanobacterial compounds produced by Sphingomonas sp. M-17. J. Antibiot., 56, 655-657. Strain(s): 45, 81, 230, 592 PubMed: 14513910 DOI: 10.7164/antibiotics.56.655 Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55. Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection Strain(s): 19, 21, 22, 25, 26, 27, 29, 32, 34, 35, 37, 38, 40, 44, 45, 46, 50, 51, 52, 53, 54, 55, 56, 58, 59, 60, 66, 68, 73, 74, 75, 77, 78, 79, 80, 81, 87, 89, 92, 95, 97, 99, 100, 105, 106, 107, 108, 109, 110, 111, 112, 120, 127, 128, 129, 130, 131, 132, 133, 134, 135, 137, 139, 144, 152, 153, 154, 156, 157, 158, 159, 162, 163, 164, 165, 166, 167, 168, 171, 177, 186, 189, 192, 193, 197, 202, 203, 204, 205, 207, 208, 209, 210, 211, 212, 214, 215, 216, 217, 224, 226, 230, 231, 232, 242, 243, 244, 245, 248, 259, 263, 267, 287, 288, 290, 294, 295, 297, 299, 300, 302, 303, 305, 306, 308, 309, 310, 312, 313, 329, 334, 337, 338, 340, 341, 342, 349, 351, 359, 361, 362, 375, 378, 379, 380, 382, 384, 385, 390, 394, 396, 398, 415, 416, 419, 422, 423, 426, 427, 429, 430, 431, 433, 434, 436, 438, 440, 446, 448, 450, 451, 452, 453, 454, 455, 456, 458, 474, 478, 479, 481, 503, 505, 506, 507, 509, 510, 512, 514, 523, 524, 527, 529, 530, 531, 532, 536, 537, 538, 539, 540, 541, 542, 544, 545, 546, 564, 565, 566, 568, 570, 573, 575, 578, 579, 580, 581, 582, 584, 592, 593, 594, 595, 596, 597, 598, 610, 628, 630, 631, 635, 638, 639, 640, 641, 642, 643, 644, 645, 647, 648, 649, 650, 652, 653, 655, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 672, 685, 686, 687, 688, 689, 691, 693, 694, 717, 718, 719, 722, 723, 726, 727, 729 Yuki, Y., Yoshida, T., Hiroishi, S. 2002 Molecular detection of the toxic cyanobacteria [Yûdoku aoko no bunshi-shikibetsu to yosatsu he-no ôyô]. In Series of Fisheries Science Vol. 143, Prevention and Extermination Strategies of Harmful Algal Blooms [Suisan-gaku shirîzu 134, Yûgai-yûdoku sôrui burûmu no yobô to kujo] , Eds. by Hiroishi, S., Imai, I. & Ishimaru, T., Koseisha-Koseikaku, Tokyo, pp. 43-53 (in Japanese). Strain(s): 73, 81, 87, 88, 89, 99, 100, 105, 106, 108, 109, 110, 111, 112, 204, 230, 299 Kasai, F., Takamura, N., Hatakeyama, S. 1993 Effects of simetryne on growth of various freshwater algal taxa. Environ. Pollut., 79, 77-83. Strain(s): 35, 44, 73, 77, 137, 230, 231, 275, 280 DOI: 10.1016/0269-7491(93)90180-V

Reference

Asukabe, H., Akahori, S., Ueno, E., Nakayama, T., Yamashita, R., Arii, S., Harada, K., Imanishi, S. Y. 2020 Cyanobacterial classification with the toxicity using MALDI Biotyper. J. Am. Soc. Mass Spectrom., 31, 1572-1578.
Keywords: cyanobacteria; MALDI Biotyper; microcystins; profiling
Strain(s): 27, 42, 73, 81, 87, 89, 100, 101, 102, 103, 104, 105, 107, 111, 112, 207, 208, 230, 266, 298, 416, 433, 478, 510, 512, 592, 593, 604, 611, 808, 809, 835, 843, 846, 847, 905, 928, 931, 932, 981, 989, 1025, 1043, 1058, 1061, 1064, 1068, 1069, 1071, 1076, 1085, 1099, 1101, 1105, 1113, 1115, 1126, 1133, 1134, 1135, 1144, 1157, 1215, 1642, 1652, 1668, 1672, 1673, 1678, 1698, 2087, 2131, 2412, 3708, 3733, 3734, 3736, 3755, 3756, 3757
PubMed: 32501712
DOI: 10.1021/jasms.0c00148

Hodoki, Y., Ohbayashi, K., Kobayashi, Y., Okuda, N., Nakano, S. 2012 Detection and identiﬁcation of potentially toxic cyanobacteria: Ubiquitous distribution of Microcystis aeruginosa and Cuspidothrix issatschenkoi in Japanese lakes. Harmful Algae, 16, 49-57.
Keywords: Anatoxin-a; Automated rRNA intergenic spacer analysis (ARISA); Cuspidothrix issatschenkoi; Cyanotoxin; Microcystin; Microcystis aeruginosa
Strain(s): 40, 77, 111, 230, 512, 843, 905, 928, 1645, 1669, 1950
DOI: 10.1016/j.hal.2012.01.003

Ioki, M., Ohkoshi, M., Nakajima, N., Nakahira-Yanaka, Y., Watanabe, M. M. 2012 Isolation of herbicide-resistant mutants of Botryococcus braunii. Bioresour. Technol., 109, 300-303.
Keywords: Botryococcus braunii; Paraquat; Methyl viologen; Basta; Glufosinate
Strain(s): 35, 230
PubMed: 21906932
DOI: 10.1016/j.biortech.2011.07.101

Ishihara, S., Horio, T., Kobara, Y., Yokoyama, A. 2006 Evaluation of herbicide effects on microalgal cells by flow cytometric analysis. J. Weed Sci. Tech., 51, 239-248 (in Japanese with English summary).
Keywords: herbicide; flow cytometry; algae; morphological change; recoverability; ecological risk assessment
Strain(s): 230
DOI: 10.3719/weed.51.239

Kim, B.-H., Oh, H.-M., Lee, Y.-K., Choi, G.-G., Ahn, C.-y., Yoon, B.-D., Kim, H.-S., 2006 Simple method for RNA preparation from cyanobacteria. J. Phycol., 42, 1137-1141.
Keywords: bead–phenol–chloroform method; cyanobacteria; RNA extraction; xanthogenate–SDS–phenol method
Strain(s): 230
DOI: 10.1111/j.1529-8817.2006.00263.x

Hirabayashi, R. & Imamura, N. 2003 Action mechanism of a selective anti-cyanobacterial compound, argimicin A. J. Antibiotics, 56, 154-159.
Strain(s): 45, 81, 204, 230, 592
PubMed: 12715875
DOI: 10.7164/antibiotics.56.154

Yamaguchi, T., Kobayashi, Y., Adachi, K., Imamura, N. 2003 Argimicins B and C, new anti-cyanobacterial compounds produced by Sphingomonas sp. M-17. J. Antibiot., 56, 655-657.
Strain(s): 45, 81, 230, 592
PubMed: 14513910
DOI: 10.7164/antibiotics.56.655

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19, 21, 22, 25, 26, 27, 29, 32, 34, 35, 37, 38, 40, 44, 45, 46, 50, 51, 52, 53, 54, 55, 56, 58, 59, 60, 66, 68, 73, 74, 75, 77, 78, 79, 80, 81, 87, 89, 92, 95, 97, 99, 100, 105, 106, 107, 108, 109, 110, 111, 112, 120, 127, 128, 129, 130, 131, 132, 133, 134, 135, 137, 139, 144, 152, 153, 154, 156, 157, 158, 159, 162, 163, 164, 165, 166, 167, 168, 171, 177, 186, 189, 192, 193, 197, 202, 203, 204, 205, 207, 208, 209, 210, 211, 212, 214, 215, 216, 217, 224, 226, 230, 231, 232, 242, 243, 244, 245, 248, 259, 263, 267, 287, 288, 290, 294, 295, 297, 299, 300, 302, 303, 305, 306, 308, 309, 310, 312, 313, 329, 334, 337, 338, 340, 341, 342, 349, 351, 359, 361, 362, 375, 378, 379, 380, 382, 384, 385, 390, 394, 396, 398, 415, 416, 419, 422, 423, 426, 427, 429, 430, 431, 433, 434, 436, 438, 440, 446, 448, 450, 451, 452, 453, 454, 455, 456, 458, 474, 478, 479, 481, 503, 505, 506, 507, 509, 510, 512, 514, 523, 524, 527, 529, 530, 531, 532, 536, 537, 538, 539, 540, 541, 542, 544, 545, 546, 564, 565, 566, 568, 570, 573, 575, 578, 579, 580, 581, 582, 584, 592, 593, 594, 595, 596, 597, 598, 610, 628, 630, 631, 635, 638, 639, 640, 641, 642, 643, 644, 645, 647, 648, 649, 650, 652, 653, 655, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 672, 685, 686, 687, 688, 689, 691, 693, 694, 717, 718, 719, 722, 723, 726, 727, 729

Yuki, Y., Yoshida, T., Hiroishi, S. 2002 Molecular detection of the toxic cyanobacteria [Yûdoku aoko no bunshi-shikibetsu to yosatsu he-no ôyô]. In Series of Fisheries Science Vol. 143, Prevention and Extermination Strategies of Harmful Algal Blooms [Suisan-gaku shirîzu 134, Yûgai-yûdoku sôrui burûmu no yobô to kujo] , Eds. by Hiroishi, S., Imai, I. & Ishimaru, T., Koseisha-Koseikaku, Tokyo, pp. 43-53 (in Japanese).
Strain(s): 73, 81, 87, 88, 89, 99, 100, 105, 106, 108, 109, 110, 111, 112, 204, 230, 299

Kasai, F., Takamura, N., Hatakeyama, S. 1993 Effects of simetryne on growth of various freshwater algal taxa. Environ. Pollut., 79, 77-83.
Strain(s): 35, 44, 73, 77, 137, 230, 231, 275, 280
DOI: 10.1016/0269-7491(93)90180-V

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