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Strain number NIES-255  
Phylum Chlorophyta  
Class Prasinophyceae  
Scientific name Monomastix minuta Skuja  
Synonym -----  
Former name  
Common name  
Locality (Date of collection) Tsuchiura Ibaraki Japan (1983-07-22)  
Latitude / Longitude 36.081096 / 140.16337 
Habitat (Isolation source) Freshwater (Pond water)  
History < Suda, Shoichiro  
Isolator (Date of isolation) Suda, Shoichiro (1983-07-22)  
Identified by Suda, Shoichiro  
State of strain Subculture; Unialgal; Clonal; Axenic[2013 Dec]  
Culture condition
(Preculture condition)
Medium:  C  
Temperature:  20 C
Light intensity:  24-39 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information 16S-ITS1-tRNA-Ile-tRNA-Ala-23S ( FN563091 ) , 18S-ITS1-5.8S-ITS2-28S ( FN562446 )  
Cell size (min - max) 3 - 12 μm  
Organization Unicellular; Flagellate 
Characteristics  
Other strain no. Other strain no. : SIS-Mono  
Remarks  
Movie  
Reference
Hamana, K., Niitsu, M., Hayashi, H. 2013 Occurrence of homospermidine and thermospermine as a cellular polyamine in unicellular chlorophyte and multicellular charophyte green algae J. Gen. Appl. Micobiol., 59, 313-319.
Keywords: green alga; homospermidine; polyamine; thermospermine
Strain(s): 1815116025529630636036364072283614091410141114191837216421993374 

Marin, B., Melkonian, M. 2010 Molecular phylogeny and classification of the Mamiellophyceae class. nov. (Chlorophyta) based on sequence comparisons of the nuclear- and plastid-encoded rRNA operons. Protist, 161, 304-336.
Keywords: prasinophytes; SSU rDNA; LSU rDNA; ITS2; systematics; freshwater; Monomastigales; Dolichomastigales; Mamiellales; Monomastigaceae; Dolichomastigaceae; Crustomastigaceae; Bathycoccaceae
Strain(s): 252255360483 
PubMed: 20005168
DOI: 10.1016/j.protis.2009.10.002

Nowack, E. C. M., Podola, B., Melkonian, M. 2005 The 96-well twin-layer system: a novel approach in the cultivation of microalgae. Protist, 156, 239-251.
Keywords: cultivation; culture collections; cell immobilisation; microalgae; microtiter plates; filter plates
Strain(s): 255483485 
DOI: 10.1016/j.protis.2005.04.003

Hamana, K., Aizaki T., Arai, E., Saito, A., Uchikata, K., Ohnishi, H. 2004 Distribution of norspermidine s a cellular polyamine within micro green algae including non-photosynthetic achlorophyllous Polytoma, Polytomella, Prototheca and Helicosporidium. J. Gen. Appl. Microbiol., 50, 289-295.
Keywords: Chlorophyta; green alga; Helicosporidium; norspermidine; polyamine; Polytoma; Polytomella; Prototheca
Strain(s): 18251254255296329483486536 

Kasai, F., Takamura, N., Hatakeyama, S. 1993 Effects of simetryne on growth of various freshwater algal taxa. Environ. Pollut., 79, 77-83.
Strain(s): 35447377102137151230231255275280298 

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