Strain number NIES-69  
Phylum Streptophyta  
Class Charophyceae  
Scientific name Closterium peracerosum-strigosum-littorale complex  
Synonym -----  
Former name  
Common name Desmid  
Locality (Date of collection) Lake Teganuma Abiko Chiba Japan (1974-06-**)  
Latitude / Longitude 35.8581459719 / 140.031447988 
Habitat (Isolation source) Freshwater  
History < Watanabe, Makoto M.  
Isolator (Date of isolation) Watanabe, Makoto M. (1974-06-**)  
Identified by Watanabe, Makoto M.  
State of strain Subculture; Unialgal; Clonal; Non-axenic  
Culture condition
(Preculture condition)
Medium:  C  
Temperature:  15 (20) C
Light intensity:  7-14 (10-17) µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  3 M (21 D)  
Gene information 18S rRNA ( AB363688 ) , 5.8S-ITS2-28S ( AB625546 )  
Cell size (min - max)  
Organization Unicellular 
Characteristics Heterothallic: Mating type (+) ; Mating group II B  
Other strain no. Other strain no. : TG-2-21  

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