Strain number NIES-686  
Phylum Chlorophyta  
Class Trebouxiophyceae  
Scientific name Chlorella vulgaris Beijerinck var. vulgaris  
Synonym  
Former name  
Common name Green alga  
Locality (Date of collection) Delft, Netherland  
Latitude / Longitude  
Habitat (Isolation source)  
History < Katagiri, Masayuki < CCAP  
Isolator (Date of isolation) Beijerinck, M. W. (1892-**-**)  
Identified by Confirmed by DNA sequencing at NIES  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2021 Jul]  
Culture condition
(Preculture condition)
Medium:  C (agar)  
Temperature:  20 (25) C
Light intensity:  4-10 (14-16) µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  3 M (21 D)  
Gene information 18S rRNA ( AB488578 )  
Cell size (min - max) - 3 μm  
Organization Unicellular 
Characteristics Authentic strain  
Other strain no. Other collection strain no. : CCAP 211/11b; IAM C-207; SAG 211-11b
Other strain no. : C-207  
Remarks Cryopreserved; Axenic 
Movie  
Reference
Jo, B.-H., Lee, C. S., Song, H.-R., Lee, H.-G., Oh, H.-M. 2014 Development of novel microsatellite markers for strain-specific identification of Chlorella vulgaris. J. Microbiol. Biotechnol., 24, 10.3354/ame01939
Keywords: Chlorella vulgaris; chloroplast; microsatellite; marker; polymorphism; sequence repeat
Strain(s): 64164268612692170 
PubMed: 24931503
DOI: 10.4014/jmb.1405.05047

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 192122232425262728293031323334353637383940414445465051525354555658596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123125127128129130131132133134135137138139144147151152153154155156157158159160162163164165166167168170171172173174175176177178179180181182183185186187188189191192193194197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268287288289290294295297298299300301302303305306308309310312313329334337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

Suda, K., Tanji, K., Hori, K., Unno, H. 1999 Evidence for a novel Chlorella virus -encoded alginate lyase. FEMS Microbiol. Lett., 180, 45-53.
Keywords: Chlorella; virus; alginate lyase; lysis
Strain(s): 6868432170 
PubMed: 10547443
DOI: 10.1111/j.1574-6968.1999.tb08776.x

Nozaki, H., Katagiri, M., Nakagawa, M., Aizawa, K., Watanabe, M. M. 1995 Taxonomic re-examination of the two strains labeled Chlorella in the Microbial Culture Collection at the National Institute for Environmental Studies (NIES-Collection). Microbiol. Cult. Coll., 11, 11-18.
Keywords: Chlorella; Graesiella emersonii comb. nov.; green algae; morphology; taxonomy
Strain(s): 226227686687688689690 

Kessler, E. & Huss, V. A. R. 1992 Comparative physiology and biochemistry and taxonomic assignment of the Chlorella (Chlorophyceae) strains of the Culture Collection of the University of Texas at Austin. J. Phycol., 28, 550-553.
Keywords: chemotaxonomy; Chlorella; Chlorophyta; UTEX culture collection; utilization for research and biotechnology
Strain(s): 6866872150215121602163216921752176 
DOI: 10.1111/j.0022-3646.1992.00550.x

Cannell, R. J. P., Owsianka, A. M., Walker, J. M. 1988 Results of a large-scale screening programme to detect antibacterial activity from freshwater algae. Br. Phycol. J., 23, 41-44.
Strain(s): 6862287 
DOI: 10.1080/00071618800650051

Yamada, T. & Sakaguchi, K. 1982 Comparative studies on Chlorella cell walls: Induction of protoplast formation. Arch. Mikrobiol., 132, 10-13.
Strain(s): 2276862159216321662168217121892352 
DOI: 10.1007/BF00690809

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