Strain number NIES-252  
Phylum Chlorophyta  
Class Nephroselmidophyceae  
Scientific name Nephroselmis astigmatica Inouye & Pienaar  
Synonym -----  
Former name  
Common name  
Locality (Date of collection) Tateyama Harbor Chiba Japan (1983-08-10)  
Latitude / Longitude 34.998 / 139.842 
Habitat (Isolation source) Marine (Seawater)  
History < Inouye, Isao  
Isolator (Date of isolation) Inouye, Isao (1983-08-10)  
Identified by Inouye, Isao  
State of strain Subculture; Unialgal; Clonal; Axenic[2014 Feb.]  
Culture condition
(Preculture condition)
Medium:  f/2ESM  
Temperature:  20 C
Light intensity:  13-16 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  42 D  
Gene information Plastid DNA ( NC_024829 KJ746600 ) , 18S rRNA ( AB158374 FN562433 ) , 16S-ITS1-tRNA-Ile-tRNA-Ala-23S ( FN563084 ) , 18S-ITS1-5.8S-ITS2-28S ( HE610145 )  
Cell size (min - max) - 8 μm  
Organization Unicellular; Flagellate 
Characteristics Red tide ; Genome decoded strain (Lemieux, C. et al. 2014)  
Other strain no. Other strain no. : 810-13  
Remarks  
Movie  
Reference
Lemieux, C., Otis, C.,Turmel, M. 2014 Six newly sequenced chloroplast genomes from prasinophyte green algae provide insights into the relationships among prasinophyte lineages and the diversity of streamlined genome architecture in picoplanktonic species. BMC Evol. Biol., 14, 857 (article ID).
Keywords: Viridiplantae; Prasinophytes; Prasinococcales; Picocystis; Picoplanktonic algae; Nephroselmis; Plastid genome; Phylogenomics; Genome reduction; trans-spliced group II intron
Strain(s): 252 

Marin, B. 2012 Nested in the Chlorellales or independent class? Phylogeny and classification of the Pedinophyceae (Viridiplantae) revealed by molecular phylogenetic analyses of complete nuclear and plastid-encoded rRNA operons. Protist, 163, 778-805.
Keywords: Marsupiomonadales; Marsupiomonas; Pedinomonas; Pedinophyceae; Resultor; Resultomonas
Strain(s): 252483 
PubMed: 22192529
DOI: 10.1016/j.protis.2011.11.004

Marin, B., Melkonian, M. 2010 Molecular phylogeny and classification of the Mamiellophyceae class. nov. (Chlorophyta) based on sequence comparisons of the nuclear- and plastid-encoded rRNA operons. Protist, 161, 304-336.
Keywords: prasinophytes; SSU rDNA; LSU rDNA; ITS2; systematics; freshwater; Monomastigales; Dolichomastigales; Mamiellales; Monomastigaceae; Dolichomastigaceae; Crustomastigaceae; Bathycoccaceae
Strain(s): 252255360483 
PubMed: 20005168
DOI: 10.1016/j.protis.2009.10.002

Alamsjah, M. A., Hirao, S., Ishibashi, F., Oda, T., Fujita, Y. 2008 Algicidal activity of polyunsaturated fatty acids derived from Ulva fasciata and U. pertusa (Ulvaceae, Chlorophyta) on phytoplankton. J. Appl. Phycol., 20, 713-720.
Keywords: Algicidal compound; Red tide phytoplankton; Ulva fasciata; Ulva pertusa
Strain(s): 137221251252254359369381462486 

Nakayama, T., Suda, S., Kawachi, M., Inouye, I. 2007 Phylogeny and ultrastructure of Nephroselmis and Pseudoscourfieldia (Chlorophyta), including the description of Nephroselmis anterostigmatica sp. nov. and a proposal for the Nephroselmidales ord. nov. Phycologia, 46, 680-697.
Keywords: 18S rDNA; hair scales; Nephroselmidales ord nov.; Nephroselmis anterostigmatica sp. nov.; phylogeny; Prasinophyceae; Pseudoscourfieldia marina; taxonomy; ultrastructure
Strain(s): 252935 
DOI: 10.2216/04-25.1

Yoshii, Y., Takaichi, S., Maoka, T., Suda, S., Sekiguchi, H., Nakayama, T., Inouye, I. 2005 Variation of siphonaxanthin series among the genus Nephroselmis (Prasinophyceae, Chlorophyta), including a novel primary methoxy carotenoid. J. Phycol., 41, 827-834.
Keywords: carotenoids; methylation; Nephroselmis; photosynthetic antenna system; Prasinophyceae; siphonaxanthin series
Strain(s): 2524834869349352309 

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