Strain number NIES-67  
Phylum Streptophyta  
Class Charophyceae  
Scientific name Closterium peracerosum-strigosum-littorale complex  
Synonym -----  
Former name  
Common name Desmid  
Locality (Date of collection) Damchan Nepal (1965-11-05)  
Latitude / Longitude  
Habitat (Isolation source) Freshwater (Pool soil)  
History < Ichimura, Terunobu  
Isolator (Date of isolation) Ichimura, Terunobu (-----)  
Identified by Ichimura, Terunobu  
State of strain Subculture; Unialgal; Clonal; Non-axenic  
Culture condition
(Preculture condition)
Medium:  C  
Temperature:  15 (20) C
Light intensity:  7-14 (10-17) µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  3 M (21 D)  
Gene information 18S rRNA ( AB363691 ) , 5.8S-ITS2-28S ( AB625554 ) , CpMADS1 mRNA ( AB091476 ) , CpMXIc mRNA ( AB197782 ) , CpPI mRNA ( AB012698 ) , ESTs(cDNA) ( AU294770-AU295959 ) , PR-IP(two subunits) ( AB000908 AB000909 ) , PR-IP ( AB000909 D21249 )  
Cell size (min - max) 90 - 125 μm  
Organization Unicellular 
Characteristics Heterothallic: Mating type (+) ; Mating group I B  
Other strain no. Other strain no. : N-31-19  
Remarks  
Movie  
Reference
Abe, J., Hirano, N., Komiya, A., Kanda, N., Fujiwara, A., Hori, S., Tsuchikane, Y., Sekimoto, H. 2016 Preparation of knockdown transformants of unicellular charophycean alga, Closterium peracerosum-strigosum-littorale complex. bio-protocol, 6,
Strain(s): 6768 
DOI: 10.21769/BioProtoc.1813

Tsuchikane, Y., Tsuchiya, M., Hindák, F., Nozaki, H., Sekimoto, H. 2012 Zygospore formation between homothallic and heterothallic strains of Closterium. Sex. Plant Reprod., 25, 1-9.
Keywords: Closterium; Conjugation; Homotallism; Heterothallism: Dexual reproduction; Zygospore
Strain(s): 5859606162636465676869702612666 

Matsumoto, H., Takechi, K., Sato, H., Takio, S., Takano, H. 2012 Treatment with antibiotics that interfere with peptidoglycan biosynthesis inhibits chloroplast division in the desmid Closterium. PLoS One, 7, e40734 (article ID).
Strain(s): 67 
DOI: 10.1371/journal.pone.0040734

Hori, S., Sekimoto, H., Abe, J. 2012 Properties of cell surface carbohydrates in sexual reproduction of the Closterium peracerosum–strigosum–littorale complex (Zygnematophyceae, Charophyta). Phycol. Res., 60, 254-260.
Keywords: cell-cell recognition; Closterium; ConcanavalinA; conjugation; lectin; Lycopersicon esculentum lectin; sexual reproduction
Strain(s): 6768 

Abe, J., Hori, S., Tsuchikane, Y., Kitao, N., Kato, M., Shiraiwa, H. 2011 Stable nuclear transformation of the Closterium complex. Plant Cell Physiol., 52, 1676-1685.
Strain(s): 6768 

Tsuchikane, Y., Ito, M., Sekimoto, H. 2008 Reproductive isolation by sex pheromones in the Closterium peracerosum-strigosum-littorale complex (Zygnematales, Charophyceae). J. Phycol., 44, 1197-1203.
Keywords: Closterium; conjugation; pheromone; reproductive isolation; sexual reproduction
Strain(s): 53545859646567686970229261 

Imaizumi, M., Sekimoto, H., Noguchi, T. 2007 Induction of sex pheromone secretion and observations of the secretory pathway in the Closterium peracerosum-strigosum-littorale complex. Plant Sci., 173, 206-212.
Keywords: Closterium peracerosum-strigosum-littorale; Immunoelectron microscopy; Protoplast release-inducing protein; Sex pheromone; Sexual reproduction; Zygote formation
Strain(s): 6768 

Takahashi, F., Okabe, Y., Nakada, T., Sekimoto, H., Ito, M., Kataoka, H. & Nozaki, H. 2007 Origins of the secondary plastids of Euglenophyta and Chlorarachniophyta as revealed by an analysis of the plastid-targeting, nuclear-encoded gene psbO. J. Phycol., 43, 1302-1309.
Keywords: alanine-X-alanine motif; Chlorarachniophyta; Euglenophyta; phylogeny; plastids; psbO; secondary endosymbiosis; transit peptide; thylakoid-targeting domain
Strain(s): 67203254381483533624656 

Akatsuka, S., Tsuchikane, Y., Fukumoto, R., Fujii, T., Sekimoto, H. 2006 Physiological characterization of the sex pheromone protoplast-release-inducing protein from the Closterium peracerosum-strigosum-littorale complex (Charophyta). Phycol. Res., 54, 116-121.
Keywords: Closterium; conjugation; mucilage; protoplast release; sex pheromone; sexual reproduction
Strain(s): 6768 

Sekimoto, H., Tanabe, Y., Tsuchikane, Y., Shirosaki, H., Fukuda,H., Demura, T., Ito, M. 2006 Gene expression profiling using cDNA microarray analysis of the sexual reproduction stage of the unicellular charophcean alga Closterium peracerosum-strigosum-littorale comples. Plant Physiol., 141, 271-279.
Strain(s): 6769 
DOI: 10.1104/pp.116.078048

Hamada, S., Sekimoto, H., Tanabe, Y., Tsuchikane, Y., Ito, M. 2006 Isolation of myosin XI genes from the Closterium peracerosum-strigosum-littorale complex and analysis of their expression during sexual reproduction. J. Plant Res., 119, 105-113.
Keywords: Closterium; Cytokinesis; Myosin XI; Real-time PCR; Sexual cell division; Sexual reproduction
Strain(s): 656768 

Tanabe, Y., Hasebe, M., Sekimoto, H., Nishiyama, T., Kitani, M., Henschel, K., Münster, T., Theißen, G., Nozaki, H., Ito, M. 2005 Characterizaion of MADS-box genes in charophycean green alga and its implication for the evolution of MADS-box genes. Proc. Nati. Acad. Sci. U. S. A., 102, 2436-2441.
Keywords: charophyve; land plants; Chara; Coleochaete; Closterium
Strain(s): 6768 
PubMed: 15699346
DOI: 10.1073/pnas.0409860102

Tsuchikane, Y., Fujii, T., Ito, M., Sekimoto, H. 2005 A sex pheromone, protoplast release-inducing protein (PR-IP) inducer, induces sexual cell division and production of PR-IP in Closterium. Plant Cell Physiol., 46, 1472-1476.
Keywords: Closterium; conjugation; pheromone; sexual cell division; sexual reproduction
Strain(s): 6768 

Sekimoto, H. 2004 Molecular characterization of a novel sex pheromone, PR-IP inducer, in Closterium peracerosum-strigosum-littorale complex. Jpn. J. Phycol., 52, Suppl., 117-122.
Keywords: Closterium; conjugation; gamete mating; sex pheromone, sexual reproduction
Strain(s): 6768 

Tsuchikane, Y., Fukumoto, R., Akatsuka, S. Fujii, T., Sekimoto, H. 2003 Sex pheromones that induce sexual cell division in the Closterium peracerosum-strigosum-littorale complex (Charophyta). J. Phycol., 39, 303-309.
Keywords: Closterium; conjugation; gametangial cell; pheromone; sexual cell division; sexual reproduction
Strain(s): 6768 

Sekimoto, H., Tanabe, Y., Takizawa, M., Ito, N., Fukumoto, R., Ito, M. 2003 Expressed sequence tags from Closterium peracerosum-strigosum-littorale complex, a unicellular charophycean alga, in the sexual reproduction process. DNA Res., 10, 147-153.
Keywords: Closterium; conjugation; EST; sexual reproduction
Strain(s): 6768 
DOI: 10.1093/dnares/10.4.147

Akatsuka, S., Sekimoto, H., Iwai, H., Fukumoto, R., Fujii, T. 2003 Mucilage secretion regulated by sex pheromones in Closterium peracerosum-strigosum-littorale complex. Plant Cell Physiol., 44, 1081-1087.
Keywords: Closterium; mucilage; sex pheromone; sexual reproduction
Strain(s): 6768 

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19212223242526272829303132333435363738394041444546505152535455565758596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123124125127128129130131132133134135137138139144147148150151152153154155156157158159160162163164165166167168169170171172173174175176177178179180181182183185186187188189191192193194195197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268271287288289290294295297298299300301302303305306308309310311312313329334336337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

Sekimoto, H. 2002 Production and secretion of a biologically active Closterium sex pheromone by Saccharomyces cerevisiae. Plant Physiol. Biochem., 40, 789-794.
Keywords: Closterium; conjugation; pheromone; PR-IP; sexual reproduction
Strain(s): 6768 

Sekimoto, H., Fukumoto, R. 1999 Sex pheromones in Closterium. Chem. Regul. Plant, 34, 305-315.
Strain(s): 64656768 

Sekimoto, H., Fukumoto, R., Dohmae, N., Takio, K. Fujii, T., Kamiya, Y. 1998 Molecular cloning of a novel sex pheromone responsible for the release of a different sex pheromone in Closterium peracerosum-strigosum-littorale complex. Plant Cell Physiol., 39, 1169-1175.
Keywords: Clostreium; conjugation; gamete; mating; sex pheromone; sexual reproduction
Strain(s): 6768 

Endo, B., Fujii, T., Kamiya, Y., Sekimoto, H. 1997 Analysis of genomic sequences encoding a sex pheromone from the Closterium peracerosum-strigosum-littorale complex. J. Plant Res., 110, 463-467.
Keywords: cis-element; Closterium; genomic DNA; protoplast-release- inducing protein (PR-IP); sex pheromone; transcription
Strain(s): 6768 

Nojiri, T., Fujii, T., Sekimoto, H. 1995 Purification and characterization of a novel sex pheromone that induces the release of another sex pheromone during sexual reproduction of the heterothallic Closterium peracerosum-strigosum-littorale complex. Plant Cell Physiol., 36, 79-84.
Keywords: Closterium; conjugation (Closterium); protoplast-release-inducing protein; pheromone
Strain(s): 6768 

Sekimoto, H., Sone, Y., Fujii, T. 1995 Biochemical, physiological, and molecular analysis of sexual isolation in the species complex Closterium peracerosum-strigosum-littorale (Chlorophyta). J. Phycol., 31, 611-615.
Keywords: Chlorophyta; Closterium peracerosum-strigosum-littorale complex; conjugation; pheromone; plotoplast-release-inducing protein (PR-IP); sexual isolation
Strain(s): 64656768 

Sekimoto, H., Sone, Y., Fujii, T. 1994 cDNA cloning of a 42-kilodalton subunit of protoplast-release-inducing protein from Closterium. Plant Physiol., 104, 1095-1096.
Strain(s): 67 

Sekimoto, H., Sone, Y., Fujii, T. 1994 A cDNA encoding a 19-kilodalton subunit of protoplast-release-inducing protein from Closterium. Plant Physiol., 105, 447.
Strain(s): 67 

Sekimoto, H., Sone, Y., Fujii, T. 1994 Regulation of expression of the genes for a sex pheromone by an inducer of the sex pheromone in the Closterium peracerosum-strigosum-littorale complex. Planta, 193, 137-144.
Keywords: Clostreium complex; inducer (of protoplast-release-inducing protein); pheromone; protoplast-release-inducing protein (PR-IP); sexual reproduction
Strain(s): 6768 

Sekimoto, H., Fujii, T. 1993 Cell-cell interaction involved in the sexual reproduction of Closterium [Mikadukimo no yûsei seisyoku ni kakawaru saibôkan jôhô kôkan]. Iden, 47 (1), 43-48 (in Japanese without English title).
Strain(s): 64656768 

Sekimoto, H., Satoh, S., Fujii, T. 1993 Analysis of binding of biotinylated protoplast-release-inducing protein that induces release of gametic protoplasts in the Closterium peracerosum-strigosum-littorale complex. Planta, 189, 468-474.
Keywords: Clostreium; conjugation (Closterium); protoplast-release; inducing protein; pheromone; receptor
Strain(s): 6768 

Sekimoto, H., Fujii, T. 1992 Analysis of gametic protoplast release in the Closterium peracerosum-strigosum-littorale complex (Chlorophyta). J. Phycol., 28, 615-619.
Keywords: biologically active glycoprotein; Closterium peracerosum-strigosum-littorale complex; conjugation; gametic protoplast release
Strain(s): 6768 

Sekimoto, H., Satoh, S., Fujii, T. 1992 Biochemical and physiological properties of a gametic protoplast-release-inducing protein in Closterium. Korean J. Phycol., 7, 121-129.
Keywords: Clostreium; conjugation; glycoprotein; protoplast release in conjugation
Strain(s): 6768 

Sekimoto, H., Fujii, T. 1991 Sexual reproduction of microalgae : its physiological and biochemical study. Cell Science, 7, 562-571 (in Japanese with English title).
Strain(s): 6768 

Sekimoto, H., Satoh, S., Fujii, T. 1990 Biochemical and physiological properties of a protein inducing protoplast release during conjugation in the Closterium peracerosum-strigosum-littorale complex. Planta, 182, 348-354.
Keywords: Clostreium; conjugation (Closterium); glycoprotein; protoplast release in conjugation
Strain(s): 6768 

Watanabe, M. M. 1977 Biosystematics in Closterium of sexual unicellular green algae and Calothrix and Spirulina of asexual filamentous blue-green algae, with special reference to the analyses of natural populations. Thesis D. Sci., Hokkaido University, 114 pp., 28 tables, 54 figs.
Strain(s): 3945465152535455565758596061626364656667686970261262266268 

Ichimura, T. 1973 The life cycle and its control in some species of Closterium, with special reference to the biological species problems. Thesis D. Sci., University of Tokyo, 69 pp., 11 tables, 40 figs.
Strain(s): 666768124125127174175176177179180181182183185198202262337338450451 

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