Strain number NIES-87  
Phylum Cyanophyta  
Class Cyanophyceae  
Scientific name Microcystis aeruginosa (Kützing) Lemmermann  
Synonym Microcystis ichthyoblabe Kützing; Microcystis novacekii (Komárek) Compère; Microcystis viridis (A.Brown) Lemmermann; Microcystis wesenbergii Komárek  
Former name Microcystis aeruginosa (Kützing) Lemmermann f. aeruginosa  
Common name Blue-green alga ; Cyanobacteria  
Locality (Date of collection) Lake Kasumigaura Ibaraki Japan (1982-09-**)  
Latitude / Longitude 36.0428477398 / 140.373233439 
Habitat (Isolation source) Freshwater (Lake water)  
History < Watanabe, Makoto M.  
Isolator (Date of isolation) Watanabe, Michiko H. (1982-09-**)  
Identified by Watanabe, Michiko H.  
State of strain Cryopreservation; Unialgal; Clonal; Axenic  
Culture condition
(Preculture condition)
Medium:  MA  
Temperature:  25 C
Light intensity:  20-30 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  20 D  
Gene information 16S rRNA ( D89031 ) , ftsZ ( AB324851 ) , glnA ( AB324930 ) , gltX ( AB325009 ) , gyrB ( AB325088 ) , pgi ( AB325167 ) , recA ( AB325246 ) , tpi ( AB325325 ) , CRISPR, ISMae21, ISMae22 ( AB644439 )  
Cell size (min - max) - 5 μm  
Organization  
Characteristics Cyanobacterial water bloom (aoko) ; ST2 (Tanabe, Y. et al. 2007)  
Other strain no. Other strain no. : K-MA-11  
Remarks Cryopreserved 
Movie  
Reference
Nakashima, Y., Egami, Y., Kimura, M., Wakimoto, T. 2016 Metagenomic analysis of the sponge Discodermia reveals the production of the cyanobacterial natural product kasumigamide by ‘Entotheonella’. PLoS One, 11, e0164468 (article ID).
Strain(s): 87 
DOI: 10.1371/journal.pone.0164468

Sun, L.W., Jiang, W.J., Sato, H., Kawachi, M., Lu, X.W. 2016 Rapid classification and identification of Microcystis aeruginosa strains using MALDI-TOF MS and polygenetic analysis. PLoS One, 11, e0156275 (article ID).
Strain(s): 44878889901001011041051071091111124786048439011043105310581061106810691070107210761077108510941099110111041105111311151126113411351144115111571170121112131215121612171218122212241230123212331239 
DOI: 10.1371/journal.pone.0156275

Akao, S., Hosoi, Y., Fujiwara, T. 2014 Utilization of water chestnut for reclamation of water environment and control of cyanobacterial blooms. Environ. Sci. Pollut. Res., 21, 2249-2255.
Keywords: Nitrogen; Phosphorus; Phenolic compounds; Potassium; Trapa natans
Strain(s): 87 
DOI: 10.1007/s11356-013-2102-2

Takeda, F., Nakano, K., Aikawa, Y., Nishimura, O., Shimada, Y., Fukuro, S., Nakazawa, T., Tanaka, H., Hayashi, N., Inamori, Y. 2014 Analysis of impact of allelopathy by submerged macrophytes simulated by ecosystem model on microalgal biomass and community. J. Jpn. Soc. Wat. Environ., 15, 15-28 (in Japanese with English summary).
Keywords: Allelopathy; Potamogeton pusillus; E cosystem model; M icroalgal community; Cladoceran
Strain(s): 3587 

Nakai, S., Asaoka, S., Okuda, T., Nishijima, W. 2014 Growth inhibition of Microcystis aeruginosa by allelopathic compounds originally isolated from Myriophyllum spicatum: temperature and light effects and evidence of possible major mechanisms. J. Chem. Eng. Japan, 47, 488-493.
Keywords: Allelopathy; Cyanobacterium; Light Intensity Effect; Photosystem II Quantum Yield; Temperature Effect
Strain(s): 87 

Nakai, S., Seto, N., Asaoka, S., Okuda, T., Nishijima, W. 2013 Impact of the allelopathic effect of a submerged macrophyte Myriophyllum spicatum on the competition between the cyanobacterium Microcystis aeruginosa and the green alga Scenedesmus obliquus. J. Jpn. Soc. Wat. Environ., 36, 85-90 (in Japanese with English summary).
Keywords: Allelopathy; Cyanobacterium; Dominance prevention; Fatty acid; Polyphenol; Submerged macrophyte
Strain(s): 872279 

Kataoka, T., Homma, T., Nakano, S., Hodoki, Y., Ohbayashi, K., Kondo, R. 2013 PCR primers for selective detection of intra-species variations in the bloom-forming cyanobacterium, Microcystis. Harmful Algae, 23, 46-54.
Keywords: Microcystis aeruginosa; Quantitative PCR; 16S-23S rDNA ITS fegion; Selective detection; Intra-species variation
Strain(s): 4487909810110210310711111229860410621070 

Yoshida, T., Takashima, Y., Tomaru Y., Shirai, Y., Takao, Y., Hiroishi, S., Nagasaki, K. 2012 Intricate interactions between the bloom-forming cyanobacterium Microcystis aeruginosa and foreign genetic elements, revealed by diversified clustered regularly interspaced short palindromic repeat (CRISPR) signatures. Appl. Environ. Microbiol., 78, 5353-5360.
Strain(s): 87102298 
PubMed: 22636003
DOI: 10.1128/AEM.00626-12

Aktas, T. S., Takeda, F., Maruo, C., Chiba, N., Nishimura, O. 2012 A comparison of zeta potentials and coagulation behaviors of cyanobacteria amd algae. Desalin. Water Treat., 48, 294-301.
Keywords: Zeta potential; Coagulation; Turbidity; Cyanobacteria; algae
Strain(s): 8713482170 

Fujii, M., Sato, Y., Ito, H., Masago, Y., Omura, T. 2012 Monosaccharide composition of the outer membrane lipopolysaccharide and O-chain from the freshwater cyanobacterium Microcystis aeruginosa NIES-87. J. Appl. Microbiol., 113, 896-903.
Keywords: chemical characterization, lipopolysaccharide, Microcystis aeruginosa, O-chain
Strain(s): 87 

Tanabe, Y., Watanabe, M. M. 2011 Local expansion of a panmictic lineage of water bloom-forming cyanobacterium Microcystis aeruginosa. PLoS One, 6, e17085 (article ID).
Strain(s): 448788899091989910010110210310410510610710810911011111229829947860484390110501051105210531054105510561057105810591060106110621063106410651066106710681069107010721075107610771085109010911092109310941095109610971098110011011102110311041105110611071108111311141115112211231124112511261127112811291130113111321133113411351136113711381139114011411142114311441145114611471148114911501151115211531155115611571159116011611162116311641170117111721173117411751176117711791180118111831184118511861209121112131214121512161217121812191220122112221223122412251226122712281229123012311232123312341235123612371238123912401241124212431244124512511252125312542088208920902091246524662467246824692470247124722473247424752476247724782479248024812482248324842485248624872488248924902491249224932494249524962546254725482549255025512552255325542555255625572558255925602561259125922593259425952596259725982599260026012602260326042605260626072608260926102611261226132617261826192620262126222623262426252626262726282629 

Takeda, F., Nakano, K., Nishimura, O., Shimada, Y., Fukuro, S., Tanaka, H., Hayashi, N. Inamori, Y. 2011 Allelopathic potential Potamogeton pusillus communitya against Microcystis aeruginosa. J. Water Environ. Technol., 9, 21-28.
Keywords: allelopathy; Microcystis aeruginosa; Potamogeton pusillus
Strain(s): 87 

Kawahara, K., Sagehashi, M., Fujii, T., Fujita, H., Hu, H.-Y., Sakoda, A. 2011 Potential of a green alga Botryococcus braunii for simultaneous water purification and biofuel production under open-air condition. J. Water Environ. Technol., 9, 29-37.
Keywords: biofuel production; Botryococcus braunii; Microcystis aeruginosa; water purification
Strain(s): 878361090 

Takeda, F., Nakano, K., Aikawa, Y., Nishimura, O., Shimada, Y., Fukuro, S., Tanaka, H., Hayashi, N. Inamori, Y. 2011 Allelopathic potential against Microcystis aeruginosa by emergent macrophytes on floating beds. J. Water Environ. Technol., 9, 371-380.
Keywords: allelopathy; Carex dispalata; floating bed; Microcystis aeruginosa; Phragmites australis; Typha domingensis
Strain(s): 87 

Nakai, S., Zou, G., Okuda, T., Tsai, T.-Y., Song, X., Nishijima, W., Okada, M. 2010 Anti-cyanobacterial allelopathic effects of plants used for artificial floating islands. Allelopathy J., 26, 113-122.
Keywords: Acorus gramineus; aquatic and terrestrial plants; artificial floating island; Canna generalis; cyanobacterium; growth inhibition; Iris pseudacorus; Lythrum salicaria; Scirpus tabernaemontani; Typha angustifolia
Strain(s): 87 

Fujise, D., Tsuji, K., Fukushima, N., Kawai, K., Harada, K. 2010 Analytical aspects of cyanobacterial volatile organic compounds for investigation of their production behavior. J. Chromatogr. A, 1217, 6122-6125.
Keywords: Cyanobacteria; Volatile organic compounds; Analysis; b-Cyclocitral; 2-Methyl-1-butanol; 3-Methyl-1-butanol; SPME
Strain(s): 87298 
DOI: 10.1016/j.chroma.2010.07.008

Tanabe, Y., Sano, T., Kasai, F., Watanabe, M. M. 2009b Recombination, cryptic clades and neutral molecular divergence of the microcystin synthetase (mcy) genes of toxic cyanobacterium Microcystis aeruginosa. BMC Evol. Biol., 9, 115 (article ID).
Strain(s): 44878889909198991001011021031041051061071081091101111122982994786048439011050105110521053105410551056105710581059106010611062106310641065106610671068106910701072107510761077108510901091109210931094109510961097109811001101110211031104110511061107110811131114111511221123112411251126112711281129113011311132113311341135113611371138113911401141114211431144114511461147114811491150115111521153115511561157115911601161116211631164117011711172120912111213121412151216121712181219122012211222122312241225122612271228122912301231123212331234123512361237123812391240124112421243124412451251125212531254246524662467246924702471247224732474247524762477247824792561 
DOI: 10.1186/1471-2148-9-115

Nakai, S., Zou, G., Song, X., Pan, Q., Zhou, S., Hosomi, M. 2008 Release of anti-cyanobacterial allelochemicals from aquatic and terrestrial plants applicable for artificial floating islands. J. Water Environ. Technol., 6, 55-63.
Keywords: allelopathy; canna; carboxylic comp.; cyanobacteria; growth inhibition; phenolic comp.; umbrella plant
Strain(s): 87 

Tanabe, Y., Kasai, F., Watanabe, M. M. 2007 Multilocus sequence typing (MLST) reveals high genetic diversity and clonal population structure of the toxic cyanobacterium Microcystis aeruginosa. Microbiology, 153, 3695-3703.
Strain(s): 44878889909198991001011021031041051061071081091101111122982994786048439019331050105110521053105410551056105710581059106010611062106310641065106610671068106910701072107510761077108510901091109210931094109510961097109811001101110211031104110511061107110811131114111511221123112411251126112711281129113011311132113311341135113611371138113911401141114211431144114511461147114811491150115111521153115511561157115911601161116211631164117011711172120912111213121412151216121712181219122012211222122312241225122612271228122912301231123212331234123512361237123812391240124112421243124412451251125212531254 

Yoshida, T., Takashima, Y., Tomaru Y., Shirai, Y., Takao, Y., Hiroishi, S., Nagasaki, K. 2006 Isolation and characterization of a cyanophage infecting the toxic cyanobacterium Microcystis aeruginosa. Appl. Environ. Microbiol., 72, 1239-1247.
Strain(s): 44879098102104111112298604 

Nakai, S., Yamada, S., Hosomi, M. 2005 Anti-cyanobacterial fatty acids released from Myriophyllum spicatum. Hydrobiologia, 543, 71-78.
Keywords: allelochemical; fatty acid; Microcystis aeruginosa; Myriophyllum spicatum
Strain(s): 87 

Nakai, S., Yamada, S., Hosomi, M. 2004 Cyanobacterial growth inhibition effects of fatty acids released from Myriophyllum spicatum. J. Jpn. Soc. Water Environ., 27, 125-130 (in Japanese with English summary).
Keywords: allelochemical; Microcystis aeruginosa; Myriophyllum spicatum; fatty acid; synergistic growth inhibition
Strain(s): 87 

Jang, M-H., Ha, K., Joo, G-J., Takamura, N. 2003 Toxin production of cyanobacteria is increased by exposure to zooplankton. Freshw. Biol., 48, 1540-1550.
Keywords: info-chemicals; microcystin production; Microcystis aeruginosa; zooplankton; zooplankton culture media filtrate
Strain(s): 44878899 

Yoshida, T., Yuki, Y., Lei, S., Chinen, H., Yoshida, M., Kondo, R., Hiroishi, S. 2003 Quantitative detection of toxic strains of the cyanobacterial genus Microcystis by competitive PCR. Microbes. Environ., 18, 16-23.
Keywords: competitive PCR; microcystin; Microcystis; peptide synthetase; toxic cyanobacteria
Strain(s): 87888990102104105106108109110111112298299604 

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19212223242526272829303132333435363738394041444546505152535455565758596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123124125127128129130131132133134135137138139144147148150151152153154155156157158159160162163164165166167168169170171172173174175176177178179180181182183185186187188189191192193194195197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268271287288289290294295297298299300301302303305306308309310311312313329334336337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

Yuki, Y., Yoshida, T., Hiroishi, S. 2002 Molecular detection of the toxic cyanobacteria [Yûdoku aoko no bunshi-shikibetsu to yosatsu he-no ôyô]. In Series of Fisheries Science Vol. 143, Prevention and Extermination Strategies of Harmful Algal Blooms [Suisan-gaku shirîzu 134, Yûgai-yûdoku sôrui burûmu no yobô to kujo] , Eds. by Hiroishi, S., Imai, I. & Ishimaru, T., Koseisha-Koseikaku, Tokyo, p. 43-53 (in Japanese without English title).
Strain(s): 738187888990919899100101102104105106108109110111112204230298299604 

Ishida, K., Murakami, M. 2000 Kasumigamide, an antialgal peptide from the cyanobacterium Microcystis aeruginosa. J. Org. Chem., 65, 5898-5900.
Strain(s): 87439 

Kondo, R., Kagiya, G., Hiroishi, S., Watanabe, M. 2000 Genetic typing of a bloom-forming cyanobacterial genus Microcystis in Japan using 16S rRNA gene sequence analysis. Plankton Biol. Ecol., 47, 1-6.
Keywords: genetic typing; 16S rDNA; Microcystis; cyanobacteria; phylogenetic analysis
Strain(s): 878889909198991001011021041051061081091101111122982996041055106110681076109010911105112211421164 

Kondo, R., Kagiya, G., Komura, M., Yuki, Y., Hiroishi, S., Watanabe, M. 2000 Molecular taxonomy of a bloom-forming cyanobacterial genus Microcystis. Bull. Plankton Soc. Japan, 47, 25-33 (in Japanese with English summary).
Keywords: cyanobacteria; Microcystis; bacterial criteria; molecular analysis
Strain(s): 6878889909198991001011021041051061081091101111122982996041055106110681076109010911105112211421164 

Kondo, R., Yoshida, T., Yuki, Y., Hiroishi, S. 2000 DNA-DNA reassociation among a bloom-forming cyanobacterial genus, Microcystis. Int. J. Syst. Evol. Microbiol., 50, 67-770.
Keywords: DNA hybridization; DNA G+C content; cyanobacteria; Microcystis
Strain(s): 878998102104111112298604 

Nakai, S., Inoue, Y., Hosomi, M., Murakami, A. 2000 Myriophyllum spicatum-released allelopathic polyphenols inhibiting growth of blue-green algae Microcystis aeruginosa. Wat. Res., 34, 3026-3032.
Keywords: Myriophyllum spicatum; allelochemical; polyphenol; synergistic growth inhibition; autoxidation; blue-green algae
Strain(s): 87 

Nakai, S., Yamane, S., Hosomi, M. 2000 Algal growth inhibition effects of Myriophyllum spicatum-releasing four allelopathic polyphenols. J. Jpn. Soc. Wat. Environ., 23, 726-730 (in Japanese with English summary).
Keywords: allelochemical; polyphenol; algae; concentration-dependent synergistic growth inhibition; autoxidation
Strain(s): 35718796489512 

Kondo, R., Kagiya, G., Hiroishi, S., Watanabe, M., Hata, Y. 1999 Rapid DNA extraction from a bloom-forming cyanobacterium. Microbes. Environ., 14, 157-161.
Keywords: DNA extraction; PCR; Microcystis; cyanobacteria
Strain(s): 878889909198991001011021041051061081091101111122982996041055106110681076109010911105112211421164 

Nakai, S., Inoue, Y., Hosomi, M., Murakami, A. 1999 Growth inhibition of blue-green algae by allelopathic effects of macrophytes. Wat. Sci. Tech., 39, 47-53.
Keywords: allelopathy; blue-green algae; coexistence; growth inhibition; macrophyte; Myriophyllum spicatum
Strain(s): 7587512 

Kondo, R., Kagiya, G., Yuki, Y., Hiroishi, S., Watanabe, M. 1998 Taxonomy of a bloom-forming cyanobacterial genus Microcystis. Nippon Suisan Gakkaishi, 64, 291-292 (in Japanese with English title).
Strain(s): 87888990919899100101102104105106107108109110111112298299604 

Li, R., Yokota, A., Sugiyama, J., Watanabe, M., Hiroki, M., Watanabe, M. M. 1998 Chemotaxonomy of planktonic cyanobacteria based on non-polar and 3-hydroxy fatty acid composition. Phycol. Res., 46, 21-28.
Keywords: 3-hydroxy fatty acids; chemotaxonomy; fatty acid; non-polar fatty acids; planktonic cyanobacteria
Strain(s): 2740447678798081878998102104111112204205207298604810821825826 

Nakai, S., Inoue, Y., Hosomi, M., Murakami, A. 1998 Concurrently inhibitory and allelopathic effects of allelochemicals secreted by Myriophyllum spicatum on growth of blue-green algae. J. Jpn. Soc. Wat. Environ., 21, 663-669 (in Japanese with English summary).
Keywords: allelopathy; algal growth; inhibition; polyphenol; concurrent action; autoxidation
Strain(s): 87 

Neilan, B. A., Jacobs, D., Goodman, A. E. 1995 Genetic diversity and phylogeny of toxic cyanobacteria determined by DNA polymorphisms within the phycocyanin locus. Appl. Environ. Microbiol., 61, 3875-3883.
Strain(s): 244041447376788081878998102107111112204207298 

Nakai, S., Inoue, H., Hosomi, M., Okada, M., Murakami, A. 1995 Control of phytoplankton growth by bioactive compounds extracted from macrophytes. J. Jpn. Soc. Wat. Environ., 18, 1012-1019 (in Japanese with English summary).
Keywords: macrophyte; algae; allelopathy; antialgal bioactive compound; growth inhibition
Strain(s): 3587 

Tominaga, H., Soejima, K., Kawagishi, S., Ashida, H., Sawa, Y., Ochiai, H. 1993 Structural organization of a cryptic plasmid, pMA1, from Microcystis aeruginosa f. aeruginosa Kutzing. Biosci. Biotechnol. Biochem., 57, 1503-1507.
Strain(s): 87 

Omura, T., Umita, T., Aizawa, J., Komatsu, Y., Ishizaki, M., Onuma, M. 1991 Accumulation of extracellular non-volatile organic acids in batch cultures of Chlorella vulgaris and Microcystis aeruginosa. Jpn. J. Water Poll. Res., 14, 615-623 (in Japanese with English summary).
Keywords: Chlorella vulgaris; Microcystis aeruginosa; batch culture; non-volatile organic acids; dissolved organic carbon
Strain(s): 87227 

Watanabe, M. M., Kasai, F., Hiwatari, T., Suda, S., Nei, T. 1984 Cryopreservation of microalgae in liquid nitrogen - Survival of algal cultures after freezing. Jpn. J. Freez. Dry., 30, 23-26 (in Japanese with English title).
Strain(s): 1819212426293139404144454650737475767780818788899091939598991001011201221311351371441512042072132572633794152219223522392579 

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