Strain number NIES-545  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Volvulina steinii Playfair  
Synonym -----  
Former name  
Common name Green alga  
Locality (Date of collection) Hayama, Kanagawa Japan (1980-12-**)  
Latitude / Longitude 35.271286 / 139.600715 
Habitat (Isolation source) Freshwater (Paddy soil)  
History < Nozaki, Hisayoshi  
Isolator (Date of isolation) Nozaki, Hisayoshi (1981-01-07)  
Identified by Nozaki, Hisayoshi  
State of strain Subculture; Unialgal; Clonal; Axenic[2014 Jan]  
Culture condition
(Preculture condition)
Medium:  VTAC  
Temperature:  20 C
Light intensity:  21 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  2 M  
Gene information 18S rRNA ( LC086366 ) , atpB ( AB044713 ) , rbcL ( AB044159 ) , psaA ( AB044222 ) , psaB ( AB044448 ) , psbC ( AB044500 )  
Cell size (min - max)  
Organization Colonial; Flagellate 
Characteristics Heterothallic ; Mating type (−) ; Crosses with NIES-546  
Other strain no. Other strain no. : 1107-5 (-)  
Nakada, T., Ito, T., Tomita, M. 2016 18S ribosomal RNA gene phylogeny of a colonial volvocalean lineage (Tetrabanaceae-Goniaceae-Volvocaceae, Volvocales, Chlorophyceae) and its close relatives. J. Jpn. Bot., 91, 345-354.
Keywords: 18S rRNA; colonial; green algae; molecular phylogeny; unicellular; Volvocales
Strain(s): 418456460474541544545564566571654666721725728735737867877879880881882883136222422246226625673388 

Nakazawa, A., Nishii, I. 2012 Amidic and acetonic cryoprotectants improve cryopreservation of volvocine green algae. CryoLetters, 33, 201-212.
Keywords: cryopreservation; dimethylformamide; hydroxyacetone; Volvocaceae; Volvox; Chlamydomonas
Strain(s): 4184685455645747287297307311361 

Ando, T., Otsuka, S., Nishiyama, M., Sendo, K., Watanabe, M. M., Matsumoto, S. 2003 Toxic effects of dichloromethane and trichloroethlene on the growth of planktonic green algae, Chlorella vulgaris NIES227, Selenastrum capricornutum NIES35, and Volvulina steinii NIES545. Microbes. Environ., 18, 43-46.
Keywords: algal growth potential (AGP); bioindicator; dichloromethane; organic solvents; trichloroethlene
Strain(s): 35227545 

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 1922232425262728293031323334353637383944454650515253545556576061626364656667707475787980818789909192939495979899100102105106107108109110111112119120123124127128129130131132133134135137138139144147148150151152153154155156157158159160162163164166167168169170171172173174175176177178179180181182183185186187188189191192193194195197198199200201202203205206207208209210211212213214215216217224226227228229230231232241242243244246248259262263266267268271287288289290294295297298299300301302303305306308309310311312313329336337338339340341342349351359360361362375378379380382384385390394396397415418419422423424426427429430431432433434436437438447448449450451452453457458459464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540542543544545546564565566567568570572573574575576577579580581584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653655656657658659660661662663664665666667672685686687688689690692693694718719720723724726727728729 

Nozaki, H., Misawa, K., Kajita, T., Kato, M., Nohara, S., Watanabe, M. M. 2000 Origin and evolution of the colonial Volvocales (Chlorophyceae) as inferred from multiple, chloroplast gene sequences. Mol. Phylogenet. Evol., 17, 256-268.
Strain(s): 418474545564566572574577653666720724727728730731734736851872882884886888889890893895896898 

Nozaki, H., Hara, Y., Kasaki, H. 1987 Light and electron microscopy of pyrenoids and species delimitation in Volvulina (Chlorophyta, Volvocaceae). J. Phycol., 23, 359-364.
Keywords: Chlorophyta; culture age; pyrenoid; pyrenoid development; taxonomy; ultrastructure; Volvocaceae; Volvulina pringsheimii; Volvulina steinii
Strain(s): 545896897898 

Nozaki, H. 1982 Morphology and reproduction of Japanese Volvulina steinii (Chlorophyta, Volvocales). J. Jpn. Bot., 57, 105-113, pls. 3-4.
Strain(s): 545546896897898 

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