Strain number NIES-215  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Pediastrum simplex Meyen  
Synonym  
Former name  
Common name Green alga  
Locality (Date of collection) Lake Biwa Shiga Japan (1982-07-**)  
Latitude / Longitude 35.2852741059 / 136.08878954 
Habitat (Isolation source) Freshwater (Lake water)  
History < Watanabe, Michiko H.  
Isolator (Date of isolation) Watanabe, Michiko H. (1982-07-**)  
Identified by Watanabe, Michiko H.  
State of strain Cryopreservation; Unialgal; Clonal; Axenic  
Culture condition
(Preculture condition)
Medium:  C  
Temperature:  20 C
Light intensity:  8-15 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  2 M  
Gene information  
Cell size (min - max) - 20 μm  
Organization Colonial 
Characteristics  
Other strain no. Other strain no. : B-P-18  
Remarks Cryopreserved; Axenic 
Movie  
Reference
Naito, K., Suzuki, M., Mito, S., Hasegawa, H., Matsui, M., Imai, I. 2006 Effects of the substances secreted from Closterium aciculare (Charophyceae, Chlorophyta) on the growth of freshwater phytoplankton under iron-deficient conditions. Plankton Benthos Res., 1, 191-199.
Keywords: Closterium aciculare; freshwater phytoplankton; growth; iron-complexing ligand; iron-deficient conditions
Strain(s): 104215248333528 
DOI: 10.3800/pbr.1.191

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19212223242526272829303132333435363738394041444546505152535455565758596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123124125127128129130131132133134135137138139144147148150151152153154155156157158159160162163164165166167168169170171172173174175176177178179180181182183185186187188189191192193194195197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268271287288289290294295297298299300301302303305306308309310311312313329334336337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

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