Strain number NIES-3669  
Phylum Chlorophyta  
Class Chloropicophyceae  
Scientific name Chloroparvula pacifica Lopes dos Santos, Noël & Eikrem  
Synonym  
Former name  
Common name  
Locality (Date of collection) Pacific Ocean (Oyashio region) Japan (2012-10-03)  
Latitude / Longitude 42.1634 / 145.0674 
Habitat (Isolation source) Marine (Seawater)  
History < Noël, Mary-Hélène  
Isolator (Date of isolation) Noël, Mary-Hélène (2013-02-08)  
Identified by Identified by DNA analysis at NIES  
State of strain Subculture; Unialgal; Clonal; Axenic[2020 Dec]  
Culture condition
(Preculture condition)
Medium:  ESM  
Temperature:  20 C
Light intensity:  22-24 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  25 D  
Gene information Mitochondrial DNA ( MK086011 ) , Plastid DNA ( MK085995 ) , 16S rRNA ( KU843560 ) , 18S rRNA ( KU843574 ) , ITS1-5.8S-ITS2-28S ( MF077486 )  
Cell size (min - max)  
Organization Unicellular 
Characteristics Genome decoded strain (Turmel et al. 2019) ; Authentic strain (Lopes dos Santos et al. 2017) ; Clade VIIB1  
Other strain no. Other collection strain no. : RCC4656
Other strain no. : MH 509  
Remarks Axenic 
Movie  
Reference
Turmel, M., Lopes Dos Santos, A., Otis, C., Sergerie, R., Lemieux, C. 2019 Tracing the evolution of the plastome and mitogenome in the Chloropicophyceae uncovered convergent tRNA gene losses and a variant plastid genetic code. Genome Biol. Evol., 11, 1275–1292.
Keywords: prasinophyte green algae; Picocystis salinarum; chloroplast genome; mitochondrial genome; phylogenomic analyses; AUA codon reassignment
Strain(s): 27552758366938353849 
PubMed: 30937436
DOI: 10.1093/gbe/evz074

Lopes dos Santos, A., Pollina, T., Gourvil, P., Corre, E., Marie, D., Garrido, J. L., Rodríguez, F., Noël, M.-H., Vaulot, D., Eikrem, W. 2017 Chloropicophyceae, a new class of picophytoplanktonic prasinophytes. Sci Rep, 7, 14019 (article ID).
Strain(s): 2755275627583667366836693670367138353849 
PubMed: 10.3775/jie.96.352
DOI: 10.1038/s41598-017-12412-5

Lopes dos Santos, A., Gourvil, P., Tragin, M., Noël, M.-H., Decelle, J., Romac, S., Vaulot, D. 2016 Diversity and oceanic distribution of prasinophytes clade VII, the dominant group of green algae in oceanic waters. ISME J., 11, 512-528.
Strain(s): 275527562758366836693670367138353849 
PubMed: 27779617
DOI: 10.1038/ismej.2016.120

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