Strain number NIES-104  
Phylum Cyanophyta  
Class Cyanophyceae  
Scientific name Microcystis aeruginosa (Kützing) Lemmermann  
Synonym Microcystis ichthyoblabe Kützing; Microcystis novacekii (Komárek) Compère; Microcystis viridis (A.Brown) Lemmermann; Microcystis wesenbergii Komárek  
Former name Microcystis wesenbergii Komárek  
Common name Blue-green alga ; Cyanobacteria  
Locality (Date of collection) The Imperial Palace Chiyoda-ku Tokyo Japan (1982-11-**)  
Latitude / Longitude 35.681958 / 139.755369 
Habitat (Isolation source) Freshwater (Moat water)  
History < Watanabe, Makoto M.  
Isolator (Date of isolation) Watanabe, Michiko H. (1982-11-**)  
Identified by Watanabe, Michiko H.  
State of strain Cryopreservation; Unialgal; Clonal; Axenic  
Culture condition
(Preculture condition)
Medium:  CBMA  
Temperature:  25 C
Light intensity:  13-16 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information 16S rRNA ( AB023266 AJ133174 ) , ftsZ ( AB324860 ) , gyrB ( AB325097 ) , glnA ( AB324939 ) , gyrB ( AB014989 ) , gltX ( AB325018 ) , pgi ( AB325176 ) , recA ( AB325255 ) , tpi ( AB325334 )  
Cell size (min - max) - 5 μm  
Organization  
Characteristics Cyanobacterial water bloom (aoko) ; ST11 (Tanabe et al. 2007)  
Other strain no. Other strain no. : MW-H1  
Remarks Cryopreserved; Axenic 
Movie  
Reference
Tanabe, Y., Hodoki, Y., Sano, T., Tada, K., Watanabe, MM. 2018a Adaptation of the freshwater bloom-forming cyanobacterium Microcystis aeruginosa to brackish water is driven by recent horizontal transfer of sucrose genes. Front. Microbiol., 9, 1150 (article ID).
Keywords: Microcystis; bloom; salt tolerance; sucrose; genomics; brackish water; horizontal gene trasnfer; ecotype
Strain(s): 4487888990919899100101102103104105107108109112298299478604843901105010521059106310671069107010751076108510931094109811001101110211041105110611071108111311141115112211281130113111321134113911411142114311441150117011741175117611771179118111831185121112131214121512321234123812391252208820902091246524662467246824692470247124722473247524762477247824792480248124822483248424852486248724882489249024912492249424952546254725482549255025512552255325542555255625572558255925602561259225942595259625972598259926002601260226032604260526062607260826102611261226132617261826192620262126222623262426252626262826294234 
PubMed: 29922255
DOI: 10.3389/fmicb.2018.01150

Sun, L.W., Jiang, W.J., Sato, H., Kawachi, M., Lu, X.W. 2016 Rapid classification and identification of Microcystis aeruginosa strains using MALDI-TOF MS and polygenetic analysis. PLoS One, 11, e0156275 (article ID).
Strain(s): 44878889901001011041051071091111124786048439011043105310581061106810691070107210761077108510941099110111041105111311151126113411351144115111571170121112131215121612171218122212241230123212331239 
PubMed: 27227555
DOI: 10.1371/journal.pone.0156275

Tanabe, Y., Watanabe, M. M. 2011 Local expansion of a panmictic lineage of water bloom-forming cyanobacterium Microcystis aeruginosa. PLoS One, 6, e17085 (article ID).
Strain(s): 4487888990919899100101102103104105106107108109110111112298299478604843901105010511052105310541055105610571058105910601061106210631064106510661067106810691070107210751076107710851090109110921093109410951096109710981100110111021103110411051106110711081113111411151122112311241125112611271128112911301131113211331134113511361137113811391140114111421143114411451146114711481149115011511152115311551156115711591160116111621163116411701171117211731174117511761177117911801181118311841185118612091211121312141215121612171218121912201221122212231224122512261227122812291230123112321233123412351236123712381239124012411242124312441245125112521253125420882089209020912465246624672468246924702471247224732474247524762477247824792480248124822483248424852486248724882489249024912492249324942495249625422543254425452546254725482549255025512552255325542555255625572558255925602561259125922593259425952596259725982599260026012602260326042605260626072608260926102611261226132617261826192620262126222623262426252626262726282629 
PubMed: 21390221
DOI: 10.1371/journal.pone.0017085

Tanabe, Y., Sano, T., Kasai, F., Watanabe, M. M. 2009b Recombination, cryptic clades and neutral molecular divergence of the microcystin synthetase (mcy) genes of toxic cyanobacterium Microcystis aeruginosa. BMC Evol. Biol., 9, 115 (article ID).
Strain(s): 44878889909198991001011021031041051061071081091101111122982994786048439011050105110521053105410551056105710581059106010611062106310641065106610671068106910701072107510761077108510901091109210931094109510961097109811001101110211031104110511061107110811131114111511221123112411251126112711281129113011311132113311341135113611371138113911401141114211431144114511461147114811491150115111521153115511561157115911601161116211631164117011711172120912111213121412151216121712181219122012211222122312241225122612271228122912301231123212331234123512361237123812391240124112421243124412451251125212531254246524662467246924702471247224732474247524762477247824792561 
PubMed: 19463155
DOI: 10.1186/1471-2148-9-115

Tanabe, Y., Kasai, F., Watanabe, M. M. 2007 Multilocus sequence typing (MLST) reveals high genetic diversity and clonal population structure of the toxic cyanobacterium Microcystis aeruginosa. Microbiology, 153, 3695-3703.
Strain(s): 44878889909198991001011021031041051061071081091101111122982994786048439019331050105110521053105410551056105710581059106010611062106310641065106610671068106910701072107510761077108510901091109210931094109510961097109811001101110211031104110511061107110811131114111511221123112411251126112711281129113011311132113311341135113611371138113911401141114211431144114511461147114811491150115111521153115511561157115911601161116211631164117011711172120912111213121412151216121712181219122012211222122312241225122612271228122912301231123212331234123512361237123812391240124112421243124412451251125212531254 

Yoshida, T., Takashima, Y., Tomaru Y., Shirai, Y., Takao, Y., Hiroishi, S., Nagasaki, K. 2006 Isolation and characterization of a cyanophage infecting the toxic cyanobacterium Microcystis aeruginosa. Appl. Environ. Microbiol., 72, 1239-1247.
Strain(s): 44879098102104111112298604 
PubMed: 16461672
DOI: 10.1128/AEM.72.2.1239-1247.2006

Naito, K., Suzuki, M., Mito, S., Hasegawa, H., Matsui, M., Imai, I. 2006 Effects of the substances secreted from Closterium aciculare (Charophyceae, Chlorophyta) on the growth of freshwater phytoplankton under iron-deficient conditions. Plankton Benthos Res., 1, 191-199.
Keywords: Closterium aciculare; freshwater phytoplankton; growth; iron-complexing ligand; iron-deficient conditions
Strain(s): 104215248333528 
DOI: 10.3800/pbr.1.191

Seo, P-S., Yokota, A. 2003 The phylogenetic relationships of cyanobacteria inferred from 16S rRNA, gyrB, rpoC1 and rpoD1 gene sequences. J. Gen. Appl. Micobiol., 49, 191-203.
Keywords: cyanobacteria; gyrB; phylogeny; protein evolution; rpoC1; rpoD1; 16S rDNA
Strain(s): 19212225391042093209521283277 
DOI: 10.2323/jgam.49.191

Yoshida, T., Yuki, Y., Lei, S., Chinen, H., Yoshida, M., Kondo, R., Hiroishi, S. 2003 Quantitative detection of toxic strains of the cyanobacterial genus Microcystis by competitive PCR. Microbes Environ., 18, 16-23.
Keywords: competitive PCR; microcystin; Microcystis; peptide synthetase; toxic cyanobacteria
Strain(s): 87888990102104105106108109110111112298299604 
DOI: 10.1264/jsme2.18.16

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19212223242526272829303132333435363738394041444546505152535455565758596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123124125127128129130131132133134135137138139144147148150151152153154155156157158159160162163164165166167168169170171172173174175176177178179180181182183185186187188189191192193194195197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268271287288289290294295297298299300301302303305306308309310311312313329334336337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

Yuki, Y., Yoshida, T., Hiroishi, S. 2002 Molecular detection of the toxic cyanobacteria [Yûdoku aoko no bunshi-shikibetsu to yosatsu he-no ôyô]. In Series of Fisheries Science Vol. 143, Prevention and Extermination Strategies of Harmful Algal Blooms [Suisan-gaku shirîzu 134, Yûgai-yûdoku sôrui burûmu no yobô to kujo] , Eds. by Hiroishi, S., Imai, I. & Ishimaru, T., Koseisha-Koseikaku, Tokyo, p. 43-53 (in Japanese without English title).
Strain(s): 738187888990919899100101102104105106108109110111112204230298299604 

Lyra, C., Suomalainen, S., Gugger, M., Vezie, C., Sundman, P., Paulin, L., Sivonen, K. 2001 Molecular characterization of planktic cyanobacteria of Anabaena, Aphanizomenon, Microcystis and Planktothrix genera. Int. J. Syst. Evol. Microbiol., 51, 13-526.
Keywords: cyanobacteria; 16S rRNA gene; RFLP; sequencing; REP; ERIC
Strain(s): 41447379818998102104 
DOI: 10.1099/00207713-51-2-513

Kondo, R., Kagiya, G., Hiroishi, S., Watanabe, M. 2000 Genetic typing of a bloom-forming cyanobacterial genus Microcystis in Japan using 16S rRNA gene sequence analysis. Plankton Biol. Ecol., 47, 1-6.
Keywords: genetic typing; 16S rDNA; Microcystis; cyanobacteria; phylogenetic analysis
Strain(s): 878889909198991001011021041051061081091101111122982996041055106110681076109010911105112211421164 

Kondo, R., Kagiya, G., Komura, M., Yuki, Y., Hiroishi, S., Watanabe, M. 2000 Molecular taxonomy of a bloom-forming cyanobacterial genus Microcystis. Bull. Plankton Soc. Japan, 47, 25-33 (in Japanese with English summary).
Keywords: cyanobacteria; Microcystis; bacterial criteria; molecular analysis
Strain(s): 6878889909198991001011021041051061081091101111122982996041055106110681076109010911105112211421164 

Kondo, R., Yoshida, T., Yuki, Y., Hiroishi, S. 2000 DNA-DNA reassociation among a bloom-forming cyanobacterial genus, Microcystis. Int. J. Syst. Evol. Microbiol., 50, 767-770.
Keywords: DNA hybridization; DNA G+C content; cyanobacteria; Microcystis
Strain(s): 878998102104111112298604 

Metcalf, J. S., Bell, S. G., Codd, G. A. 2000 Production of novel polyclonal antibodies against the cyanobacterial toxin microcystin-LR and their application for the detection and quantification of microcystins and nodularin. Water Res., 34, 2761-2769.
Keywords: cyanobacteria; blue-green algae; microcystin; toxins; antibodies; immunoassays
Strain(s): 42102103104595 
DOI: 10.1016/S0043-1354(99)00429-7

Kondo, R., Kagiya, G., Hiroishi, S., Watanabe, M., Hata, Y. 1999 Rapid DNA extraction from a bloom-forming cyanobacterium. Microbes Environ., 14, 157-161.
Keywords: DNA extraction; PCR; Microcystis; cyanobacteria
Strain(s): 878889909198991001011021041051061081091101111122982996041055106110681076109010911105112211421164 
DOI: 10.1264/jsme2.14.157

Otsuka, S., Suda, S., Li, R., Watanabe, M., Oyaizu, H., Matsumoto, S., Watanabe, M. M. 1999 Phylogenetic relationships between toxic and non-toxic strains of the genus Microcystis based on 16S to 23S internal transcribed spacer sequence. FEMS Mircobiol. Lett., 172, 15-21.
Keywords: cyanobacteria; Microcystis; internal transcribed spacer; phylogeny; microcystin
Strain(s): 44104111105410551058106710681072108510861090109110921105111511171143 

Kondo, R., Kagiya, G., Yuki, Y., Hiroishi, S., Watanabe, M. 1998 Taxonomy of a bloom-forming cyanobacterial genus Microcystis. Nippon Suisan Gakkaishi, 64, 291-292 (in Japanese with English title).
Strain(s): 87888990919899100101102104105106107108109110111112298299604 

Li, R., Yokota, A., Sugiyama, J., Watanabe, M., Hiroki, M., Watanabe, M. M. 1998 Chemotaxonomy of planktonic cyanobacteria based on non-polar and 3-hydroxy fatty acid composition. Phycol. Res., 46, 21-28.
Keywords: 3-hydroxy fatty acids; chemotaxonomy; fatty acid; non-polar fatty acids; planktonic cyanobacteria
Strain(s): 2740447678798081878998102104111112204205207298604810821825826 
DOI: 10.1111/j.1440-1835.1998.tb00093.x

Ward, C. J., Beattie, K. A., Lee, E. Y. C., Codd, G. A. 1997 Colorimetric protein phosphatase inhibition assay of laboratory strains and natural blooms of cyanobacteria: comparisons with high-performance liquid chromatographic analysis for microcystins. FEMS Mircobiol. Lett., 153, 465-473.
Keywords: Microcystin; Protein phosphatase; Cyanobacteria; High-performance liquid chromatography
Strain(s): 42102103104298595 
PubMed: 9271876
DOI: 10.1111/j.1574-6968.1997.tb12611.x

Soma, Y., Imaizumi, T., Yagi, K., Kasuga, S. 1993 Estimation of algal succession in lake water using HPLC analysis of pigments. Can. J. Fish. Aquat. Sci., 50, 1142-1146.
Strain(s): 44738188104111233234333351 

Yamaguchi, K., Murakami, M., Okino, T. 1989 Screening of angiotensin-converting enzyme inhibitory activities in microalgae. J. Appl. Phycol., 1, 271-275.
Keywords: angiotensin converting enzyme; inhibitor; microalgae; peptide; screening
Strain(s): 14100102104114 

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