株番号 NIES-227  
門名 Chlorophyta  
綱名 Trebouxiophyceae 
学名 Chlorella vulgaris Beijerinck var. vulgaris  
シノニム  
以前使われていた種名  
一般名 クロレラ ; 緑藻  
地名(採集年月) 日本  
緯度/経度  
生息環境(分離源) 陸域  
分離後の履歴 ← IAM;  
分離者(分離年月) 渡辺篤  
同定者 福島博; Confirmed at NIES by DNA sequencing  
株の状態 凍結保存; 無菌[2014 Feb]; 単藻; クローン  
培養条件
(前培養条件)
培地名:  C (寒天)  
温度: 20 (25) ℃
光強度: 4-10 (15-19) µmol photons/m2/sec,  明暗周期: 10L:14D
継代培養周期: 3 M (14 D)  
遺伝子情報 16S rRNA ( AJ242754 AJ242771 ) , 18S rRNA ( AJ242756 AB488575 ) , 18S-ITS1-5.8S-ITS2 ( AB162910 ) , 28S rRNA ( AB237642 ) , CO1 ( D63763 AB011523 ) , psaA ( AB260919 ) , rbcL ( AB260909 )  
特性  
サイズ (min - max) 2 - 10 μm  
体制 単細胞性 
その他の株番号 他のコレクション番号:IAM C-30
 
注意事項 凍結保存; 無菌  
動画  
文献
Suzuki, N., Sanada, T., Terashima, C., Suzuki, T., Arai, T., Saitou, S., Kawashima, T., Mizutani, A., Saitou, T., Nakata, K., Katsumata, K., Kondo, T., Yuasa, M., Fujishima, A. 2017 Systematic studies of TiO2-based photocatalysts anti-algal effects on Chlorella vulgaris. J. Appl. Electrochem., 47, 197-203.
キーワード: Titanium dioxide; Photocatalyst; Anti-algal effect; Hydrogen peroxide
Strain(s): 227 
DOI: 10.1007/s10800-016-1031-4

Shen, X.-F., Liu, J.-J., Chauhan, A. S., Hu, H., Ma, L.-L., Lam, P.K.S., Zeng, R. J. 2016 Combining nitrogen starvation with sufficient phosphorus supply for enhanced biodiesel productivity of Chlorella vulgaris fed on acetate. Algal Research, 17, 261-267.
キーワード: Heterotrophic cultivation; Lipid productivity; Biodiesel; Microalgae; Nutrient regulation; pH control
Strain(s): 227 
DOI: 10.1016/j.algal.2016.05.018

Shen, X.-F., Chu, F.-F., Lam, P.K.S., Zeng, R.J. 2015 Biosynthesis of high yield fatty acids from Chlorella vulgaris NIES-227 under nitrogen starvation stress during heterotrophic cultivation. Water Res., 81, 294-300.
キーワード: Microalgae; Nitrogen starvation; Heterotrophic cultivation; Fatty acid content; Fatty acid yield; Glucose
Strain(s): 227 
PubMed: 26081436
DOI: 10.1016/j.watres.2015.06.003

Ioki, M., Ohkoshi, M., Nakajima, N., Nakahira-Yanaka, Y., Watanabe, M. M. 2012 Isolation of herbicide-resistant mutants of Botryococcus braunii. Biores. Technol., 109, 300-303.
キーワード: Botryococcus braunii; Paraquat; Methyl viologen; Basta; Glufosinate
Strain(s): 351192272302235 
DOI: 10.1016/j.biortech.2011.07.101

Ueda, H., Otsuka, S., Senoo, K. 2010 Bacterial communities constructed in artificial consortia of bacteria and Chlorella vulgaris. Microbes Environ., 25, 36-40.
キーワード: Chlorella vulgaris; algal-bacterial association; bacteria; co-cultivation; consortium
Strain(s): 227 
PubMed: 21576850
DOI: 10.1264/jsme2.ME09177

Kato, Y., Imamura, N., 2008 Effect of calcium ion on uptake of amino acids by symbiotic Chlorella F36-ZK isolated from Japanese Paramecium bursaria. Plant Sci., 174, 88-96.
キーワード: Paramecium bursaria; Chlorella; Calcium; Divalent cation; Amino acid transport; Symbiosis
Strain(s): 22721572540 
DOI: 10.1016/j.plantsci.2007.10.001

Hoshina, R., Imamura, N. 2008 Eu-Chlorella large subunit rDNA sequences and group I introns in ribosomal DNA of the paramecian symbiotic alga NC64A. Phycol. Res., 56, 21-32.
キーワード: Chlorella vulgaris; group I intron; intron phylogeny; intron transposition; large subunit ribosomal DNA; NC64A
Strain(s): 227 
DOI: 10.1111/j.1440-1835.2008.00481.x

Kato, Y., Ueno, S., Imamura, N. 2006 Studies on the nitrogen utilization of endosymbiotic algae isolated from Japanese Paramecium bursaria. Plant Sci., 170, 481-486.
キーワード: Endosymbiont; Paramecium bursaria; Nitrogen utilization; Chlorella
Strain(s): 22725402541 
DOI: 10.1016/j.plantsci.2005.09.018

Shitanda, I., Takada, K., Sakai, Y., Tatsuma, T. 2005 Compact amperometric algal biosensors for the evaluation of water toxicity. Anal. Chim. Acta, 530, 191-197.
キーワード: amperometric biosensor; Chlorella vulgaris; algae; alginate gel; polyion complex; herbicides
Strain(s): 227 
DOI: 10.1016/j.aca.2004.09.073

押見誠則, 藤本尚志, 大西章博, 鈴木昌治, 秋葉道宏, 国包章一 2005 かび臭産生藍藻類Phormidium tenueを溶解する細菌の分離と溶藻特性に関する研究 (Isolation of bacteria lysing musty odor producing alga Phormidium tenue and its lytic characteristics.) 水環境学会誌 (J. Jpn. Soc. Wat. Environ.), 28, 379-384 (in Japanese with English summary).
キーワード: alga-lytic bacteria; musty odor; Phormidium tenue; Pseudomonas KA6
Strain(s): 96211227244298512817 
DOI: 10.2965/jswe.28.379

Sato, R., Fujishima, H., Itabashi, Y. 2003 Analysis of algal phosphatidylglycerols by chiral phase high-performane liquid chromatography. Bull. Fish. Sci. Hokkaido Univ., 54, 7-12 (in Japanese with English summary).
キーワード: phosphatidylglycerol; diastereomer; Enteromorpha sp.; Chlorella vulgaris; Heterosigma akashiwo; spinach; parsley; green tea; chiral phase HPLC; 3,5-dinitrophenylurethane
Strain(s): 227 

Ando, T., Otsuka, S., Nishiyama, M., Sendo, K., Watanabe, M. M., Matsumoto, S. 2003 Toxic effects of dichloromethane and trichloroethlene on the growth of planktonic green algae, Chlorella vulgaris NIES227, Selenastrum capricornutum NIES35, and Volvulina steinii NIES545. Microbes Environ., 18, 43-46.
キーワード: algal growth potential (AGP); bioindicator; dichloromethane; organic solvents; trichloroethlene
Strain(s): 35227545 
DOI: 10.1264/jsme2.18.43

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
キーワード: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19212223242526272829303132333435363738394041444546505152535455565758596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123124125127128129130131132133134135137138139144147148150151152153154155156157158159160162163164165166167168169170171172173174175176177178179180181182183185186187188189191192193194195197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268271287288289290294295297298299300301302303305306308309310311312313329334336337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

Kuramoto, T., Iwamoto, K., Izumi, M., Kirihata, M., Yoshizako, F. 1999 Asymmetric reduction of ethyl 2-methyl 3-oxobutanoate by Chlorella. Biosci. Biotechnol. Biochem., 63, 598-601.
キーワード: Chlorella; asymmetric reduction; ethyl 2-methyl 3-oxobutanoate; (2S, 3S)-ethyl 2-methyl 3-hydroxybutanoate; (2S, 3R)-ethyl 2-methyl 3-hydroxybutanoate
Strain(s): 226227 
PubMed: 10227152
DOI: 10.1271/bbb.63.598

Inagaki, Y., Ehara, M., Watanabe, K. I., Hayashi-Ishimaru, Y., Ohama, T. 1998 Directionally evolving genetic code: the UGA codon from stop to tryptophan in mitochondria. J. Mol. Evol., 47, 378-384.
キーワード: COXI phylogenetic tree; UGA codon; deviant genetic code; directional codon reassignment
Strain(s): 12227274 
PubMed: 9767683
DOI: 10.1007/PL00006395

Watanabe, K. I., Ehara, M., Inagaki, Y., Ohama, T. 1998 Distinctive origins of group I introns found in the COXI genes of three green algae. Gene, 213, 1-7.
キーワード: Group I intron; Chlorella vulgaris; Scenedesmus quadricauda; Protosiphon botryoides; cytochrome c oxidase subunit I; intron homing; frameshift
Strain(s): 96227 
DOI: 10.1016/S0378-1119(98)00235-2

Hayashi-Ishimaru, Y., Ohama, T., Kawatsu, Y., Nakamura, K., Osawa, S. 1996 UAG is a sense codon in several chlorophycean mitochondria. Curr. Genet., 30, 29-33.
キーワード: deviant genetic code; COXI; Chlorophyceaen mitochondria; release factor
Strain(s): 96209227415 

Liu, C. B., Lin, L. P., Su, Y. C. 1996 Utilization of Chlorella vulgaris for uptake of nitrogen, phosphorus and heavy metals. J. Chin. Agr. Chem. Soc., 34, 331-343 (in Chinese with English summary).
キーワード: Chlorella vulgaris; heavy metals; nitrogen; phosphorus
Strain(s): 227 

Nozaki, H., Katagiri, M., Nakagawa, M., Aizawa, K., Watanabe, M. M. 1995 Taxonomic re-examination of the two strains labeled Chlorella in the Microbial Culture Collection at the National Institute for Environmental Studies (NIES-Collection). Microbiol. Cult. Coll., 11, 11-18.
キーワード: Chlorella; Graesiella emersonii comb. nov.; green algae; morphology; taxonomy
Strain(s): 226227686687688689690 

Kasai, F., Hatakeyama, S. 1993 Herbicide susceptibility in two green algae, Chlorella vulgaris and Selenastrum capricornutum. Chemosphere, 27, 899-904.
Strain(s): 35227 
DOI: 10.1016/0045-6535(93)90019-2

Morimoto, T., Murakami, N., Nagatsu, A., Sakakibara, J. 1993 Studies on glycolipids. VII. Isolation of two new sulfoquinovosyl diacylglycerols from the green alga Chlorella vulgaris. Chem. Pharm. Bull., 41, 1545-1548.
キーワード: sulfoquinovosyl diacylglycerol (SQDG); glyceroglycolipid; green alga; Chlorella vulgaris; branched fatty acid
Strain(s): 227 
DOI: 10.1248/cpb.41.1545

Watanabe, Y., Ohmura, N., Saiki, H. 1992 Microbial CO2 fixation. 2. Isolation and determination of cultural characteristics of Chlorella strains which function under CO2 enriched atmosphere. CRIEPI Kenkyû-Hôkoku, U92014, 1-21 (in Japanese with English summary).
キーワード: climate change; CO2; CO2 fixation; microalgae; Chlorella
Strain(s): 27459294122158204226227 

Omura, T., Umita, T., Aizawa, J., Komatsu, Y., Ishizaki, M., Onuma, M. 1991 Accumulation of extracellular non-volatile organic acids in batch cultures of Chlorella vulgaris and Microcystis aeruginosa. Jpn. J. Water Poll. Res., 14, 615-623 (in Japanese with English summary).
キーワード: Chlorella vulgaris; Microcystis aeruginosa; batch culture; non-volatile organic acids; dissolved organic carbon
Strain(s): 87227 

Yoshizako, F., Nishimura, A., Chubachi, M., Horii, T., Ueno, T. 1991 Bioconversion of cyclohexaneacetic acid to monohydroxycyclo-hexaneacetic acids by Chlorella pyrenoidosa Chick. J. Ferment. Bioeng., 72, 343-346.
Strain(s): 226227 

Kawanobe, K. 1990 A report in the completion of the research work [Chôsa kenkyû syûryô hôkoku]. Unpublished report in Kankyô Hozen Kyôkai, 3 pp. (in Japanese without English title).
Strain(s): 35227 

Iehara, M. 1990 Kinetic analysis of the growth of Chlorella vulgaris. Biotech. Bioeng., 36, 198-206.
Strain(s): 227 
PubMed: 18595068
DOI: 10.1002/bit.260360212

Ubekôsan Co. Ltd. 1988 Effects of UR-158 on the algal mortality [UR-158 no sassôkôka]. Unpublished report in Ubekôsan Co. Ltd, 3 pp. (in Japanese without English title).
Strain(s): 32227 

Yamada, T., Sakaguchi, K. 1982 Comparative studies on Chlorella cell walls: Induction of protoplast formation. Arch. Mikrobiol., 132, 10-13.
Strain(s): 2276862159216321662168217121892352 

Oh-hama, T., Senger, H. 1978 Spectral effectiveness in chlorophyll and 5-amino levulinic acid formation during regreening of glucose-bleached cells of Chlorella protothecoides. Plant Cell Physiol., 19, 1295-1299.
キーワード: ALA synthesis; blue light effect; Chlorella protothecoides; chlorophyll formation
Strain(s): 227 

Ichimura, T., Itoh, T. 1977 17. Preservation methods of microalgae (I) [17. Bisaisôrui no hozonhô (I)]. In Preservation methods of microorganisms [Biseibutsu Hozonhô], Ed. by Nei, T., University of Tokyo Press, Tokyo, p. 355-373 (in Japanese without English title).
Strain(s): 19212223242728303132333436373839404144454647484950122123127131144152173175179180181182183185198200203206217226227241266267268271295309310334338379436448450455527 

Maruyama, K. 1977 Classification of Chlorella strain by infrared absorption spectra of cell samples. Bot. Mag. Tokyo, 90, 67-77.
Strain(s): 2262276856872151216321702175 

Maruyama, K. 1977 Classification of Chlorella strains by cell appearance and group sera. Bot. Mag. Tokyo, 90, 57-66.
Strain(s): 2262276852151216321702175 

Watanabe, A. 1960 List of algal strains in collection at the Institute of Applied Microbiology, University of Tokyo. J. Gen. Appl. Microbiol., 6, 283-292.
Strain(s): 192122232425262829374749226227 

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