株番号 NIES-2235  
門名 Chlorophyta  
綱名 Chlorophyceae 
学名 Chlamydomonas reinhardtii Dangeard  
シノニム Chlamydomonas morieri Dangeard 1888; Chlamydomonas pseudodebaryana Brabez 1941  
以前使われていた種名  
一般名 緑藻 ; クラミドモナス ; コナミドリムシ  
地名(採集年月)  
緯度/経度  
生息環境(分離源)  
分離後の履歴 ← IAM ← 坪由宏 (1959) ← Sager (1954) ← Smith (1953)  
分離者(分離年月)  
同定者  
株の状態 継代培養; 無菌[2014 Mar]; 単藻; クローン  
培養条件
(前培養条件)
培地名:  C (寒天)  
温度: 20 ℃
光強度: 2-3 µmol photons/m2/sec,  明暗周期: 10L:14D
継代培養周期: 6 M  
遺伝子情報 Total genome information ( http://chlamy.pmb.lif.kyoto-u.ac.jp/chlamybase ) , EST information ( http://est.kazusa.or.jp/en/plant/chlamy/EST/index.html ) , Amt protein mRNA(putative) ( AF509497 ) , CAH1 ( D90206 AB026126 ) , CAH2 ( X54488 ) , CrDES ( AB239769 ) , GCS1 ( AB206813 ) , mmp1 ( AB058411 AB058413 ) , mmp2 ( AB058412 AB058414 ) , mmp3 ( AB108850 ) , NSG1 ( AB167472 ) , NSG6 ( AB167474 ) , NSG7 ( AB167475 ) , NSG11 ( AB167476 ) , NSG13 ( AB167477 ) , NSG17 ( AB167478 ) , RECA ( AB048829 ) , SIG1 mRNA ( AB049220 ) , 18S rRNA ( AB701550 )  
特性 走光性 ; デンプン生産 (Kuchitsu, K. et al. 1988) ; ゲノム解読株 ; 交配型 (−) ; NIES-2239 (IAM C-562)と交配 ; 餌料 (Liu, X., et al. 2016)  
サイズ (min - max)  
体制 単細胞性; 鞭毛性 
その他の株番号 他のコレクション番号:IAM C-9
その他の株番号:Tsubo R (−)  
注意事項  
動画  
文献
Liu, X., Syuhei, B. 2017 Effects of acclimatization on metabolic plasticity of Eodiaptomus japonicus (Copepoda: Calanoida) determined using an optical oxygen meter. J. Plankton Res., 39, 111-121.
キーワード: temperature acclimatization; metabolic rates; oxygen consumption rates; zooplankton; optical oxygen meter
Strain(s): 2822235 
DOI: 10.1093/plankt/fbw084

Hirashima, T., Toyoshima, M., Moriyama, T., Nakamura, Y., Sato, N. 2017 Characterization of phosphoethanolamine-N-methyltransferases in green algae. Biochem. Biophys. Res. Commun., 488, 141-146.
キーワード: Green algal evolution; Chlamydomonas; Phosphatidylcholine biosynthesis; Phosphoethanolamine-N-methyltransferase
Strain(s): 22022207221222352242 
PubMed: 2848352
DOI: 10.1016/j.bbrc.2017.05.026

Liu, X., Beyrend-Dur, D., Dur, G., Ban, S. 2015 Combined effects of temperature and food concentration on growth and reproduction of Eodiaptomus japonicus (Copepoda: Calanoida) from Lake Biwa (Japan). Freshw. Biol., 60, 2003-2018.
キーワード: copepod dynamics; egg production; Eodiaptomus japonicus; individual growth rates; temperature–food effects
Strain(s): 2822235 
DOI: 10.1111/fwb.12626

Kong, F., Yamasaki, T., Kurniasih, S. D., Hou, L., Li, X., Ivanova, N., Okada, S., Ohama, T. 2015 Robust expression of heterologous genes by selection marker fusion system in improved Chlamydomonas strains. Biotech. Bioeng., 120, 239-245.
キーワード: Transgene silencing; Position effect; Squalene synthase-like gene; Botryococcus braunii; Methyltransferase gene
Strain(s): 2235 
PubMed: 25660568
DOI: 10.1016/j.jbiosc.2015.01.005

Wang, L., Yamano, T., Kajikawa, M., Hirono, M., Fukuzawa, H. 2014 Isolation and characterization of novel high-CO2-requiring mutants of Chlamydomonas reinhardtii. Photosynth. Res., 121, 175-184.
キーワード: Chlamydomonas reinhardtii; Carbon-concentrating mechanism; CO2-signal transduction; High-CO2-requiring mutants
Strain(s): 2235 
DOI: 10.1007/s11120-014-9983-x

Liu, X., Beyrend-Dur, D., Dur, G., Ban, S. 2014 Effects of temperature on life history traits of Eodiaptomus japonicus (Copepoda: Calanoida) from Lake Biwa (Japan). Limnology, 15, 85-97.
キーワード: Life history traits; Temperature effects; Eodiaptomus japonicus; Copepod dynamics; Individual variability
Strain(s): 2822235 
DOI: 10.1007/s10201-013-0417-9

Yumoto, K., Kasai, F., Kawachi, M. 2013 Taxonomic re-examination of Chlamydomonas strains maintained in the NIES-Collection. Microbiol. Cult. Coll., 29, 1-12.
Strain(s): 1584374384408849681021102210481733184818492201220222032204220622072208220922102211221222132214221522162218221922202222222322242225222822292230223122332234223522362237223922402241224222432246224722482314231523162317231823192320232123222323232424622463249925752576257725782579 

Yamano, T., Iguchi, H. Fukuzawa, H. 2013 Rapid transformation of Chlamydomonas reinhardtii without cell-wall removal. J. Biosci. Bioeng., 115, 691-694.
キーワード: Chlamydomonas reinhardtii; Electroporation; Square electric pulse; Transformation
Strain(s): 2235 
PubMed: 23333644
DOI: 10.1016/j.jbiosc.2012.12.020

Ioki, M., Ohkoshi, M., Nakajima, N., Nakahira-Yanaka, Y., Watanabe, M. M. 2012 Isolation of herbicide-resistant mutants of Botryococcus braunii. Biores. Technol., 109, 300-303.
キーワード: Botryococcus braunii; Paraquat; Methyl viologen; Basta; Glufosinate
Strain(s): 351192272302235 
DOI: 10.1016/j.biortech.2011.07.101

Fujii, K., Nakashima, H., Hashidzume, Y. 2010 Isolation of folate-producing microalgae, from oligotrophic ponds in Yamaguchi, Japan. J. Appl. Microbiol., 108, 1421-1429.
キーワード: B-vitamins; Chlamydomonas; Chlorococcum; folate; microalgae; thiamine
Strain(s): 217222352848284928502851 
PubMed: 19796121
DOI: 10.1111/j.1365-2672.2009.04542.x

Yamano, T., Miura, K., Fukuzawa, H. 2008 Expression analysis of genes associated with the induction of the carbon-concentrating mechanism in Chlamydomonas reinhardtii. Plant Physiol., 147, 340-354.
Strain(s): 2235 
PubMed: 18322145
DOI: 10.1104/pp.107.114652

Kajikawa, M., Yamato, K. T., Kohzu, Y., Shoji, S., Sakai, Y., Fukuzawa, H. 2006 A front-end desaturase from the green alga Chlamydomonas reinhardtii produces pinolenic and coniferonic acids by ω13 desaturation. Plant Cell Physiol., 47, 64-73.
キーワード: Chlamydomonas reinhardtii; Δ5-unsaturated bis-methylene-interrupted fatty acids; fatty acid desaturase; Pichia pastoris; tobacco plant
Strain(s): 2235 
PubMed: 16267098
DOI: 10.1093/pcp/pci224

Yoshioka, S., Taniguchi, F., Miura, K., Inoue, T., Yamano, T., Fukuzawa, H. 2004 The novel Myb transcription factor LCR1 regulates the CO2-responsive gene Cah1, encoding a periplasmic carbonic anhydrase in Chlamydomonas reinhardtii. Plant Cell, 16, 1466-1477.
Strain(s): 2235 
PubMed: 15155888
DOI: 10.1105/tpc.021162

Miura, K., Yamano, T., Yoshioka, S., Kohinata, T., Inoue, Y., Taniguchi, F., Asamizu, E., Nakamura, Y., Tabata, S., Yamato, S. T., Ohyama, K., Fukuzawa, H. 2004 Expression profiling-based identification of CO2-responsive genes regulated by CCM1 controlling a carbon-concentrating mechanism in Chlamydomonas reinhardtii. Plant Physiol., 135, 1595-1607.
Strain(s): 2235 
PubMed: 15235119
DOI: 10.1104/pp.104.041400

Asamizu, E., Nakamura, Y., Miura, K., Fukuzawa, H., Fujiwara, S., Hirono, M., Iwamoto, K., Matsuda, Y., Minagawa, J., Shimogawara, K., Takahashi, Y., Tabata, S. 2004 Establishment of publicly available cDNA material and information resource of Chlamydomonas reinhardtii (Chlorophyta), to facilitate gene function analysis. Phycologia, 43, 722-726.
Strain(s): 2235 
DOI: 10.2216/i0031-8884-43-6-722.1

Abe, J., Kubo, T., Takagi, Y., Saito, T., Miura, K., Fukuzawa, H., Matsuda, Y. 2004 The transcriptional program of synchronous gametogenesis in Chlamydomonas reinhardtii. Curr. Genet., 46, 304-315.
Strain(s): 2235 
PubMed: 15459796
DOI: 10.1007/s00294-004-0526-4

Kubo, T., Abe, J.,  Saito, T., Matsuda, Y. 2002 Genealogical relationships among laboratory strains of Chlamydomonas reinhardtii as inferred from matrix metalloprotease genes. Curr. Genet., 41, 115-122.
キーワード: Chlamydomonas reinhardtii; Chlamydomonas smithii; matrix metalloprotease genes; molecular phylogeny
Strain(s): 2235 

Kubo, T., Saito, T., Fukuzawa, H., Matsuda, Y. 2001 Two tandemly-located matrix metalloprotease genes with different expression patterns in the Chlamydomonas sexual cell cycle. Curr. Genet., 40, 136-143.
キーワード: cell wall degradation; Chlamydomonas reinhardtii; gametolysin; zygote-specific gene
Strain(s): 2235 

Asamizu, E., Miura, K., Kucho, K., Inoue, Y., Fukuzawa, H., Ohyama, K., Nakamura, Y., Tabata, S. 2000 Generation of expressed sequence tags from low-CO2 and high-CO2 adapted cells of Chlamydomonas reinhardtii. DNA Res., 7, 305-307.
キーワード: Chlamydomonas reinhardtii; cDNA; EST; CO2 stress
Strain(s): 2235 

Asamizu, E., Nakamura, Y., Sato, S., Fukuzawa, H., Tabata, S. 1999 A large scale structure analysis of cDNAs in a unicellular green alga, Chlamydomonas reinhardtii. I. Generation of 3433 non-redundant expressed sequence tags. DNA Res., 6, 369-373.
キーワード: green alga; Chlamydomonas reinhardtii; cDNA; EST; normalized library; similarity search
Strain(s): 2235 

Ban, S. 1992 Effects of photoperiod, temperature, and population density on induction of diapause egg production in Eurytemora affinis (Copepoda: Calanoida) in Lake Ohnuma, Hokkaido, Japan. J. Crustacean Biol., 12, 361-367.
Strain(s): 2822235 
DOI: 10.1163/1937240X92X00247

Tachiki, A., Fukuzawa, H., Miyachi, S. 1992 Characterization of carbonic anhydrase isozyme CA2, which is the CAH2 gene product, in Chlamydomonas reinhardtii. Biosci. Biotechnol. Biochem., 56, 794-798.
Strain(s): 2235 

Fukuzawa, H., Ishida, S., Miyachi, S. 1991 cDNA cloning and gene expression of carbonic anhydrase in Chlamydomonas reinhardtii. Can. J. Bot., 69, 1088-1096.
キーワード: photosynthesis; light regulation; zinc; CO2-concentrating mechanism; intracellular processing
Strain(s): 2235 

Ehara, T., Ogasawara, Y., Osafune, T. 1990 Behavior of chloroplast nucleoids during the cell cycle of Chlamydomonas reinhardtii (Chlorophyta) in synchronized culture. J. Phycol., 26, 317-323.
キーワード: cell cycle; Chlamydomons; chloroplast division; DAPI; nucleoid
Strain(s): 2235 

Kashino, Y., Enami, I., Satoh, K., Katoh, S. 1990 Immunological cross-reactivity among corresponding proteins of photosystems I and II from widely divergent photosynthetic organisms. Plant Cell Physiol., 31, 479-488.
キーワード: antiserum; cross-reactivity; immunoblotting; photosystem; protein
Strain(s): 209321382235 
DOI: 10.1093/oxfordjournals.pcp.a077935

Tsuzuki, M., Ohnuma, E., Sato, N., Takaku, T., Kawaguchi, A. 1990 Effects of CO2 concentration during growth on fatty acid composition in microalgae. Plant Physiol., 93, 851-856.
Strain(s): 482093213821602235 
DOI: 10.1104/pp.93.3.851

Fukuzawa, H., Fujiwara, S., Yamamoto, Y., Dionisio-Sese, M. L., Miyachi, S. 1990 cDNA cloning, sequence, and expression of carbonic anhydrase in Chlamydomonas reinhardtii: Regulation by environmental CO2 concentration. Proc. Nati. Acad. Sci. U. S. A., 87, 4383-4387.
Strain(s): 2235 

Fujiwara, S., Fukuzawa, H., Tachiki, A., Miyachi, S. 1990 Structure and differential expression of two genes encoding carbonic anhydrase in Chlamydomonas reinhardtii. Proc. Nati. Acad. Sci. U. S. A., 87, 9779-9783.
Strain(s): 2235 

Kamo, T., Shimogawara, K., Fukuzawa, H., Muto, S., Miyachi, S. 1990 Subunit constitution of carbonic anhydrase from Chlamydomonas reinhardtii. Eur. J. Biochem., 192, 557-562.
Strain(s): 2235 

Dionisio-Sese, M. L., Fukuzawa, H., Miyachi, S. 1990 Light-induced carbonic anhydrase expression in Chlamydomonas reinhardtii. Plant Physiol., 94, 1103-1110.
Strain(s): 2235 
PubMed: 16667803
DOI: 10.1104/pp.94.3.1103

Tsuzuki, M., Miyachi, S. 1989 The function of carbonic anhydrase in aquatic photosynthesis. Aquatic Botany, 34, 85-104.
Strain(s): 20932138216021732235 

Dionisio, M. L., Tsuzuki, M., Miyachi, S. 1989 Light requirement for carbonic anhydrase induction in Chlamydomonas reinhardtii. Plant Cell Physiol., 30, 207-213.
キーワード: blue light; carbonic anhydrase; Chlamydomonas reinhardtii; enzyme induction; light intensity
Strain(s): 2235 

Dionisio, M. L., Tsuzuki, M., Miyachi, S. 1989 Blue light induction of carbonic anhydrase activity in Chlamydomonas reinhardtii. Plant Cell Physiol., 30, 215-219.
キーワード: blue light; carbonic anhydrase; Chlamydomonas reinhardtii; enzyme induction; photoreceptor
Strain(s): 2235 

Toguri, T., Muto, S., Mihara, S., Miyachi, S. 1989 Synthesis and degradation of carbonic anhydrase in a synchronized culture of Chlamydomonas reinhardtii. Plant Cell Physiol., 30, 533-539.
キーワード: carbonic anhydrase synthesis; Chlamydomonas reinhardtii; synchronous culture
Strain(s): 2235 

Kuchitsu, K., Tsuzuki, M., Miyachi, S. 1988 Changes of starch localization within the chloroplast induced by changes in CO2 concentration during growth of Chlamydomonas reinhardtii. Independent regulation of pyrenoid starch and stroma starch. Plant Cell Physiol., 29, 1269-1278.
キーワード: Chlamydomonas (Chlorophyta); chloroplast; CO2 concentration; pyrenoid; starch; stroma
Strain(s): 2235 

Kuchitsu, K., Tsuzuki, M., Miyachi, S. 1988 Characterization of the pyrenoid isolated from unicellular green alga Chlamydomonas reinhardtii. Particulate form of RuBisCO protein. Protoplasma, 144, 17-24.
キーワード: Chlamydomonas; pyrenoid; ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO); stroma
Strain(s): 2235 

Yagawa, Y., Muto, S., Miyachi, S. 1988 Carbonic anhydrase of Chlamydomonas reinhardtii. Cellular distribution and subunit constitution in two strains. Plant Cell Physiol., 29, 185-188.
キーワード: carbonic anhydrase (cellular distribution); Chlamydomonas
Strain(s): 2235 

Bürger, J., Miyachi, S., Galland, P., Senger, H. 1988 Quantum requirements of photosynthetic oxygen evolution and 77K fluorescence emission spectra in unicellular green algae grown under low- and high-CO2-conditions. Botanica Acta, 101, 229-232.
Strain(s): 21732235 

Toguri, T., Muto, S., Miyachi, S. 1986 Biosynthesis and intracellular processing of carbonic anhydrase in Chlamydomonas reinhardtii. Eur. J. Biochem., 58, 443-450.
Strain(s): 2235 

Aizawa, K., Miyachi, S. 1986 Carbonic anhydrase and CO2 concentrating mechanisms in microalgae and cyanobacteria. FEMS Mircobiol. Lett., 39, 215-233.
キーワード: aquatic algae; cyanobacteria; photoautotrophs; photorespiration; photosynthesis; transport
Strain(s): 2093216021732235 

Yagawa, Y., Aizawa, K., Yang, S.-Y., Miyachi, S. 1986 Release of carbonic anhydrase from the cell surface of Chlamydomonas reinhardtii by trypsin. Plant Cell Physiol., 27, 215-221.
キーワード: carbonic anhydrase; cell surface; Chlamydomonas reinhardtii; trypsin treatment
Strain(s): 2235 

Miyachi, S., Tsuzuki, M., Aizawa, K. 1985 Factors which enhance photosynthesis in microalgae under limiting CO2 concentrations. In Kinetics of Photosynthetic Carbon Metabolism in C3-plants. II. Electron Transport, Transport of CO2, Mathematical Models, Eds. by Viil, J. et al., Akademiya Nauka, Estonia SSR, p. 88-92.
Strain(s): 21602235 

Yang, S.-Y., Tsuzuki, M., Miyachi, S. 1985 Carbonic anhydrase of Chlamydomonas: Pulification and studies on its induction using antiserum against Chlamydomonas carbonic anhydrase. Plant Cell Physiol., 26, 25-34.
キーワード: affinity chromatography; carbon dioxide concentration; carbonic anhydrase; Chlamydomonas reinhardtii; enzyme induction
Strain(s): 2235 
DOI: 10.1093/oxfordjournals.pcp.a076892

Watanabe, M. M., Kasai, F., Hiwatari, T., Suda, S., Nei, T. 1984 Cryopreservation of microalgae in liquid nitrogen - Survival of algal cultures after freezing. Jpn. J. Freez. Dry., 30, 23-26 (in Japanese with English title).
Strain(s): 1819212426293139404144454650737475767780818788899091939598991001011201221311351371441512042072132572633794152219223522392579 

Tsuzuki, M., Shimamoto, T., Yang, S. Y., Miyachi, S. 1984 Diversity in intracellular locality, nature, and function of carbonic anhydrase in verious plants. Ann. NY Acad. Sci., 429, 238-240.
Strain(s): 6872160216121622170217322352280 
DOI: 10.1111/j.1749-6632.1984.tb12341.x

Tsuzuki, M. 1983 Mode of HCO3-utilization by the cells of Chlamydomonas reinhardtii grown under ordinary air. Z. Pflanzenphysiol., 110, 29-37.
Strain(s): 216021702235 

Mihara, S., Hase, E. 1978 Studies on the vegetative life cycle of Chlamydomonas reinhardi Dangeard in synchronous culture. V. The transition point for the effect of 6-methyl purine in the cell cycle. Plant Cell Physiol., 19, 83-89.
キーワード: cell cycle; Chlamydomonas; 6-methyl purine; transition point
Strain(s): 2235 

Osafune, T., Mihara, S., Hase, E., Ohkuro, I. 1976 Electron microscope studies on the vegetative cellular life cycle of Chlamydomonas reinhardi Dangeard in synchronous culture. IV. Mitochondria in dividing cells. J. Electr. Microsc., 25, 261-269.
Strain(s): 2235 

Osafune, T., Mihara, S., Hase, E., Ohkuro, I. 1975 Electron microscope studies on the vegetative cellular life cycle of Chlamydomonas reinhardi Dangeard in synchronous culture. III. Three-dimensional structures of mitochondria in the cells at intermediate stages of the growth phase of the cell cycle. J. Electr. Microsc., 24, 247-252.
Strain(s): 2235 

Mihara, S., Hase, E. 1975 Studies on the vegetative life cycle of Chlamydomonas reinhardtii Dangeard in synchronous culture. III. Some notes on the process of zoospore liberation. Plant Cell Physiol., 16, 371-375.
Strain(s): 2235 

Mihara, S., Hase, E. 1975 Studies on the vegetative life cycle of Chlamydomonas reinhardtii Dangeard in synchronous culture. IV. Effects of 6-methyl purine on the revolution of the cell cycle. Plant Cell Physiol., 16, 1121-1130.
Strain(s): 2235 

Osafune, T., Mihara, S., Hase, E., Ohkuro, I. 1972 Electron microscope studies on the vegetative cellular life cycle of Chlamydomonas reinhardi Dangeard in synchronous culture. II. Association of mitochondria and the chloroplast at an early developmental stage. Plant Cell Physiol., 13, 981-989.
Strain(s): 2235 

Osafune, T., Mihara, S., Hase, E., Ohkuro, I. 1972 Electron microscope studies on the vegetative cellular life cycle of Chlamydomonas reinhardi Dangeard in synchronous culture. I. Some characteristics of changes in subcellular structures during the cell cycle, especially in formation of giant mitochondria. Plant Cell Physiol., 13, 211-227.
Strain(s): 2235 

Mihara, S., Hase, E. 1971 Studies on the vegetative life cycle of Chlamydomonas reinhardtii Dangeard in synchronous culture. I. Some characteristics of the cell cycle. Plant Cell Physiol., 12, 225-236.
Strain(s): 2235 

Mihara, S., Hase, E. 1971 Studies on the vegetative life cycle of Chlamydomonas reinhardtii Dangeard in synchronous culture. II. Effects of chloramphenicol and cycloheximide on the length of cell cycle. Plant Cell Physiol., 12, 237-241.
Strain(s): 2235 

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