Strain number NIES-49  
Phylum Euglenozoa  
Class Euglenophyceae  
Scientific name Euglena gracilis Klebs var. bacillaris Pringsheim  
Synonym -----  
Former name  
Common name  
Locality (Date of collection) (-----)  
Latitude / Longitude  
Habitat (Isolation source)  
History < IAM (1983);  
Isolator (Date of isolation) (-----)  
Identified by -----  
State of strain Subculture; Unialgal; Clonal; Axenic[2013 Jan]  
Culture condition
(Preculture condition)
Medium:  HUT  
Temperature:  20 (25) C
Light intensity:  2 (15-19) µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  45 D (14 D)  
Gene information  
Cell size (min - max) 40 - 50 μm  
Organization Unicellular; Flagellate 
Other strain no. Other collection strain no. : IAM E-2 (=E-10)
Aoyama, K., Miyata, N., Iwahori, K. 2005 Evalution of DNA damaging potential in landfill leachates by comet assay using Euglena gracilis. Jpn. J. Water Treat. Biol., 41, 73-81.
Keywords: comet assay; Euglena gracilis; landfill leachate; umu test
Strain(s): 49 

Iwahori, K., Miyata, N., Umeda, Y., Aoyama, K., Shimoi, K., Kinae, N. 1999 Applicability of environmental microorganisms to alkaline single cell gel electrophoresis (comet) assay. Jpn. J. Water Treat. Biol., 35, 261-270 (in Japanese with English summary).
Keywords: comet assay; DNA damage; environmental microorganisms; Euglena gracilis; cell lysis; unwinding; electrophoresis; SDS; γ-irradiation
Strain(s): 49122403 

Iwata, H., Haji, A., Hashimoto, K., Mitsuyanagi, K., Kanegae, Y. 1991 Methods for producing heat-unsoluble β-1,3-glucan [Kanetsu gyôkosei β-1,3-glucan no seizôhô]. In Nippon-koku Tokkyo-chô (JP), Kôkai Tokkyo Kôhô (A), Hei3-163102, 5 pp. (in Japanese without English title).
Strain(s): 474849 

Ichimura, T., Itoh, T. 1977 17. Preservation methods of microalgae (I) [17. Bisaisôrui no hozonhô (I)]. In Preservation methods of microorganisms [Biseibutsu Hozonhô], Ed. by Nei, T., University of Tokyo Press, Tokyo, p. 355-373 (in Japanese without English title).
Strain(s): 19212223242728303132333436373839404144454647484950122123127131144152173175179180181182183185198200203206217226227241266267268271295309310334338379436448450455527 

Watanabe, A. 1960 List of algal strains in collection at the Institute of Applied Microbiology, University of Tokyo. J. Gen. Appl. Microbiol., 6, 283-292.
Strain(s): 192122232425262829374749226227 

Nishimura, M., Huzisige, H. 1959 Studies on the chlorophyll formation in Euglena gracilis with special reference to the action spectrum of the process. J. Biochem., 46, 225-234.
Strain(s): 49 

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