Strain Data

Strain number		NIES-396
Phylum		Chlorophyta
Class		Chlorophyceae
Scientific name		Volvox aureus Ehrenberg
Synonym		Janetosphaera aurea (Ehrenberg) W.R.Shaw 1922; Volvox dioica var. lismorensis Playfair null; Volvox minor F.Stein 1854; Volvox dioicus F.J.Cohn 1875; Volvox lismorensis Playfair 1915
Former name
Common name		Green alga ; Volvocine alga
Locality (Date of collection)		Koshoku-gun, Nagano, Japan (1983-08-27)
Latitude / Longitude
Habitat (Isolation source)		Freshwater (Paddy water)
History		< Ogasawara, Yoshikazu
Isolator (Date of isolation)		Ogasawara, Yoshikazu (1983-08-29)
Identified by		Ogasawara, Yoshikazu
State of strain		Subculture; Unialgal; Clonal; Axenic[2018 Feb]
Culture condition (Preculture condition)		Medium: VT Temperature: 23 C Light intensity: 27-31 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 1 M
Gene information
Cell size (min - max)
Organization		Colonial; Flagellate
Characteristics		Phototaxis ; Homothallic
Other strain no.
Remarks		Fragile species to transportation stresses; Read and agree "How to order 4.1"; Axenic
Movie

Reference
Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55. Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection Strain(s): 19, 21, 22, 23, 25, 26, 27, 29, 31, 32, 34, 35, 36, 37, 38, 40, 44, 45, 46, 50, 51, 52, 53, 54, 55, 56, 58, 59, 60, 66, 67, 68, 73, 74, 75, 77, 78, 79, 80, 81, 87, 89, 92, 93, 95, 97, 98, 99, 100, 105, 106, 107, 108, 109, 110, 111, 112, 119, 120, 127, 128, 129, 130, 131, 132, 133, 134, 135, 137, 139, 144, 152, 153, 154, 155, 156, 157, 158, 159, 160, 162, 163, 164, 165, 166, 167, 168, 171, 177, 186, 189, 192, 193, 197, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 214, 215, 216, 217, 224, 226, 230, 231, 232, 242, 243, 244, 245, 246, 248, 259, 263, 266, 267, 268, 287, 288, 290, 294, 295, 297, 299, 300, 302, 303, 305, 306, 308, 309, 310, 312, 313, 329, 334, 337, 338, 340, 341, 342, 349, 351, 359, 361, 362, 375, 378, 379, 380, 382, 384, 385, 390, 394, 396, 398, 415, 416, 419, 422, 423, 426, 427, 429, 430, 431, 433, 434, 436, 438, 439, 440, 446, 447, 448, 450, 451, 452, 453, 454, 455, 456, 458, 460, 464, 474, 478, 479, 481, 503, 504, 505, 506, 507, 509, 510, 512, 514, 515, 522, 523, 524, 527, 529, 530, 531, 532, 536, 537, 538, 539, 540, 541, 542, 544, 545, 546, 564, 565, 566, 568, 569, 570, 572, 573, 574, 575, 578, 579, 580, 581, 582, 584, 592, 593, 594, 595, 596, 597, 598, 610, 628, 630, 631, 635, 638, 639, 640, 641, 642, 643, 644, 645, 647, 648, 649, 650, 652, 653, 655, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 672, 685, 686, 687, 688, 689, 691, 692, 693, 694, 717, 718, 719, 722, 723, 726, 727, 729 Hirata, K., Phunchindawan, M., Tukamoto, J., Goda, S., Miyamoto, K. 1996 Cryopreservation of microalgae using encapsulation-dehydration. CryoLetters, 17, 321-328. Keywords: Dunaliella tertiolecta; microalgae; cryopreservation; encapsulation-dehydration Strain(s): 8, 19, 22, 27, 32, 44, 242, 375, 396, 422

Reference

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19, 21, 22, 23, 25, 26, 27, 29, 31, 32, 34, 35, 36, 37, 38, 40, 44, 45, 46, 50, 51, 52, 53, 54, 55, 56, 58, 59, 60, 66, 67, 68, 73, 74, 75, 77, 78, 79, 80, 81, 87, 89, 92, 93, 95, 97, 98, 99, 100, 105, 106, 107, 108, 109, 110, 111, 112, 119, 120, 127, 128, 129, 130, 131, 132, 133, 134, 135, 137, 139, 144, 152, 153, 154, 155, 156, 157, 158, 159, 160, 162, 163, 164, 165, 166, 167, 168, 171, 177, 186, 189, 192, 193, 197, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 214, 215, 216, 217, 224, 226, 230, 231, 232, 242, 243, 244, 245, 246, 248, 259, 263, 266, 267, 268, 287, 288, 290, 294, 295, 297, 299, 300, 302, 303, 305, 306, 308, 309, 310, 312, 313, 329, 334, 337, 338, 340, 341, 342, 349, 351, 359, 361, 362, 375, 378, 379, 380, 382, 384, 385, 390, 394, 396, 398, 415, 416, 419, 422, 423, 426, 427, 429, 430, 431, 433, 434, 436, 438, 439, 440, 446, 447, 448, 450, 451, 452, 453, 454, 455, 456, 458, 460, 464, 474, 478, 479, 481, 503, 504, 505, 506, 507, 509, 510, 512, 514, 515, 522, 523, 524, 527, 529, 530, 531, 532, 536, 537, 538, 539, 540, 541, 542, 544, 545, 546, 564, 565, 566, 568, 569, 570, 572, 573, 574, 575, 578, 579, 580, 581, 582, 584, 592, 593, 594, 595, 596, 597, 598, 610, 628, 630, 631, 635, 638, 639, 640, 641, 642, 643, 644, 645, 647, 648, 649, 650, 652, 653, 655, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 672, 685, 686, 687, 688, 689, 691, 692, 693, 694, 717, 718, 719, 722, 723, 726, 727, 729

Hirata, K., Phunchindawan, M., Tukamoto, J., Goda, S., Miyamoto, K. 1996 Cryopreservation of microalgae using encapsulation-dehydration. CryoLetters, 17, 321-328.
Keywords: Dunaliella tertiolecta; microalgae; cryopreservation; encapsulation-dehydration
Strain(s): 8, 19, 22, 27, 32, 44, 242, 375, 396, 422

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