Strain number NIES-565  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Astrephomene perforata Nozaki  
Synonym  
Former name  
Common name Green alga  
Locality (Date of collection) Hayama, Kanagawa, Japan (1980-12-04)  
Latitude / Longitude 35.271286 / 139.600715 
Habitat (Isolation source) Freshwater (Paddy soil)  
History < Nozaki, Hisayoshi  
Isolator (Date of isolation) Nozaki, Hisayoshi (1981-06-20)  
Identified by Nozaki, Hisayoshi  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2018 Feb]  
Culture condition
(Preculture condition)
Medium:  VTAC  
Temperature:  20 C
Light intensity:  3-5 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  42 D  
Gene information  
Cell size (min - max)  
Organization Colonial; Flagellate 
Characteristics Phototaxis ; Authentic strain ; Heterothallic ; Isogamous ; Mating type (−) ; Crosses with NIES-564  
Other strain no. Other collection strain no. : UTEX 2475
Other strain no. : 1620-4-1  
Remarks Cryopreserved; Axenic 
Movie  
Reference
Nozaki, H., Mori, F., Tanaka, Y., Matsuzaki, R., Yamashita, S., Yamaguchi, H., Kawachi, K. 2023 Cryopreservation of two species of the multicellular volvocine green algal genus Astrephomene. BMC Microbiol., 23, 16 (article ID).
Keywords: Asexual cycle; Astrephomene; Cryopreservation; Cryoprotectant; Culture collection; Volvocine green algae
Strain(s): 4184195645656288538548554017 
PubMed: 36650459
DOI: 10.1186/s12866-023-02767-3

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 192122232425262728293031323334353637383940414445465051525354555658596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123125127128129130131132133134135137138139144147151152153154155156157158159160162163164165166167168170171172173174175176177178179180181182183185186187188189191192193194197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268287288289290294295297298299300301302303305306308309310312313329334337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

Nozaki, H. & Ito, M. 1994 Phylogenetic relationships within the colonial Volvocales (Chlorophyta) inferred from cladistic analysis based on morphological data. J. Phycol., 30, 353-365.
Keywords: Chlorophyta; cladistic analysis; Goniaceae; morphological data; phylogeny; sexual reproduction; Tetrabaena; Tetrabaenaceae fam. nov.; Tetrabaena socialis comb. nov.; Volvocaceae
Strain(s): 456460541565571654 
DOI: 10.1111/j.0022-3646.1994.00353.x

Nozaki, H. 1983 Morphology and taxonomy of two species of Astrephomene (Chlorophyta, Volvocales) in Japan. J. Jpn. Bot., 58, 345-352, pls. 11-13.
Strain(s): 418419564565 

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