Strain number NIES-2697  
Phylum Haptophyta  
Class Prymnesiophyceae  
Scientific name Emiliania huxleyi (Lohmann) Hay & Mohler  
Synonym Pontosphaera huxleyi Lohmann; Hymenomonas huxleyi (Lohmann) Kamptner 1930; Coccolithus huxleyi (Lohmann) Kamptner 1943; Gephyrocapsa huxleyi (Lohmann) Reinhardt 1972  
Former name  
Common name Coccolithophore  
Locality (Date of collection) Tasman Sea Australia (1998-09-01)  
Latitude / Longitude / 169.83333333 
Habitat (Isolation source) Marine (Seawater)  
History < RCC (2010);  
Isolator (Date of isolation) Probert, I. (1998-09-01)  
Identified by  
State of strain Subculture; Unialgal; Clonal; Non-axenic  
Culture condition
(Preculture condition)
Medium:  ESMK/2  
Temperature:  15 C
Light intensity:  5-11 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information CO1 ( KC404350 ) , CO1b, atp4 ( AB563780 ) , CO3 ( JN098173 KC404548 ) , 28S rRNA ( LC102344 )  
Cell size (min - max) 5 - 12 μm  
Organization Unicellular 
Characteristics Planktonic  
Other strain no. Other collection strain no. : RCC 1216
Other strain no. : AC472  
Remarks  
Movie  
Reference
Araie, H., Nakamura, H., Toney, J. L., Haigg, H. A., Plancq, J., Shiratori, T., Leavitt, P. R., Seki, O., Ishida, K-I., Sawada, K., Suzuki, I., Shiraiwa, Y. 2018 Novel alkenone-producing strains of genus Isochrysis (Haptophyta) isolated from Canadian saline lakes show temperature sensitivity of alkenones and alkenoates. Org. Geochem., 121, 89-103.
Keywords: Alkenones; Alkenone unsaturation index; Canadian salt lakes; Chemotaxonomy; Haptophytes; Isochrysis; Long-chain alkyl ketones; Paleothermometer; UK37
Strain(s): 10002697 
DOI: 10.1016/j.orggeochem.2018.04.008

Mitani, E., Nakayama, F., Matsuwaki, I., Ichi, I., Kawabata, A., Kawachi, M., Kato, M. 2017 Fatty acid composition profiles of 235 strains of three microalgal divisions within the NIES Microbial Culture Collection. Microb. Resour. Syst., 33, 19-29.
Keywords: Cryptophyta; docosahexaenoic acid; eicosapentaenoic acid; fatty acid; Haptophyta; Heterokontophyta; microalgae
Strain(s): 189141517711151452232252332342652742752762772782792802812822842933233243303333443453463473483503533723773883913954074084094134144174614624664875345485535565575585595605625875885895906036056216226236956966976986997007017027037047057067077087097107117127137147157167417657667678058379971001100210031004100510061007100910111016101710441045104610471302130313241327133013391340134913531370137513761379138313841385138613871391139213931395139813991400140116991700173018131815181618261827183118621863186418651874196319641965214221432144214521472148230023312332235123632364236523692370237625062533253425352536253725902633266826892690269126932694269626972716271727182720272227232725272627292730273127322770277127722773283928402841284228432844285928722878289028993391 

Nakamura, H., Sawada, K., Araie, H., Shiratori, T., Ishida, K-I., Suzuki, I., Shiraiwa, Y. 2016 Composition of long chain alkenones and alkenoates as a function of growth temperature in marine haptophyte Tisochrysis lutea. Org. Geochem., 99, 78-89.
Keywords: Alkenones; Chemotaxonomy; Tisochrysis; Isochrysis; UK37
Strain(s): 100025902697 
DOI: 10.1016/j.orggeochem.2016.06.006

Bendif, E. M., Probert, I., Carmichael, M., Romac, S., Hagino, K., de Vardas, C. 2014 Genetic delineation between and within the widespread coccolithophore morpho-species Emiliania huzleyi and Gephyrocapsa. J. Phycol., 50, 140-148.
Keywords: coccolithophore; DNA barcoding; Emiliania huxleyi; Gephyrocapsa oceanica; phylogeny; species complex
Strain(s): 10002697 
PubMed: 26988015
DOI: 10.1111/jpy.12147

del Campo, J., Sieracki, M.E., Molestina, R., Keeling, P., Massana, R., Ruiz-Trillo, I. 2014 The others: our biased perspective of eukaryotic genomes. Trends Ecol. Evol., 29, 252-259.
Strain(s): 100025822584258725902670267326912693269426952696269726992700275127532755275627582761336933703371 
PubMed: 24726347
DOI: 10.1016/j.tree.2014.03.006

Rokitta, S. D., de Nooijer, L. J., Trimborn, S., de Vargas, C., Rost, B., John, U. 2011 Transcriptome analyses reveal differential gene expression patterns between the life-cycle stages of Emiliania huxleyi (Haptophyta) and reflect specialization to different ecological niches. J. Phycol., 47, 829-838.
Keywords: endocytosis; life-cycle stages; microarray; quantitative RT-PCR; transcriptome profiling
Strain(s): 2697 
PubMed: 27020019
DOI: 10.1111/j.1529-8817.2011.01014.x

Reid, E. L., Worthy, C. A., Probert, I., Ali, S.T., Love, J., Napier, J., Littlechild, J. A., Somerfield, P. J., Allen , M. J. 2011 Coccolithophores: functional biodiversity, enzymes and bioprospecting. Mar. Drugs., 9, 586-602.
Keywords: functional biodiversity; bioprospecting; biocatalysis; coccolithophore
Strain(s): 2697 
DOI: 10.3390/md9040586

Langer, G., Nehrke, G., Probert, I., Ly, J., Ziveri, P. 2009 Strain-specific responses of Emiliania huxleyi to change seawater carbonate chemistry. Biogeosciences, 6, 2637-2646.
Strain(s): 2697 
DOI: 10.5194/bg-6-2637-2009

Houndan, A., Billard, C., Marie, D., Not, F., Sáez, A. G., Young, J. R., Probert, I. 2003 Holococcolithophore-heterococcolithophore (Haptophyta) life cycles: flow cytometric analysis of relative ploidy levels. Systematics and Biodiversity, 4, 453-465.
Keywords: Coccolithophore; haplo-diploid; Haptophyta; heterococcolith; holococcolith; life cycle; microalgae; ploidy
Strain(s): 26942697 

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