保存株情報

株番号		NIES-2145
門名		Heterokontophyta
綱名		Eustigmatophyceae
学名		Nannochloropsis oceanica Suda & Miyashita
シノニム
以前使われていた種名		Nannochloropsis oculata (Droop) Hibberd
一般名
地名（採集年月）
緯度/経度
生息環境（分離源）		海域
分離後の履歴		← IAM ← 原慶明（筑波大 1988) ← 福島県立水産センター
分離者（分離年月）
同定者
株の状態		継代培養; 非無菌; 単藻; クローン
培養条件（前培養条件）		培地名： ESM （寒天）温度： 20 ℃ 光強度： 5-9 µmol photons/m²/sec, 明暗周期： 10L:14D 継代培養周期： 4 M
遺伝子情報
特性		オイル（炭化水素）生産 ; トリアシルグリセロール (Nobusawa et al.)
サイズ (min - max)
体制		単細胞性
その他の株番号		他のコレクション番号：IAM ST-4
注意事項
動画

文献
Pollner, E., Farré, E. M., Bennlng, C. 2018 Advanced genetic tools enable synthetic biology in the oleaginous microalgae Nannochloropsis sp. Plant Cell Reports, 37, 1383-1399. キーワード: Nannochloropsis; Algal biotechnology; Marker-free engineering; Gene stacking; Synthetic biology; Episomes Strain(s): 2145, 2146 PubMed: 29511798 DOI: 10.1007/s00299-018-2270-0 Shimakawa, G., Murakami, A., Niwa, K., Matsuda, Y., Wada, A., Miyake, C. 2018 Comparative analysis of strategies to prepare electron sinks in aquatic photoautotrophs. Photosynth. Res., キーワード: Reactive oxygen species; P700 oxidation; Photosystem I; Seaweeds Strain(s): 705, 1959, 2138, 2145, 2147 PubMed: 29845382 DOI: 10.1007/s11120-018-0522-z Nobusawa, T., Hori, K., Mori, H., Kurokawa, K., Ohta, H. 2017 Differently localized lysophosphatidic acid acyltransferasescrucial for triacylglycerol biosynthesis in the oleaginous alga Nannochloropsis. Plant J., 90, 547–559. キーワード: Nannochloropsis; triacylglycerol (TAG); biofuels; lysophosphatidic acid acyltransferase (LPAT); lipid droplets Strain(s): 2145 PubMed: 28218992 DOI: 10.1111/tpj.13512 Mitani, E., Nakayama, F., Matsuwaki, I., Ichi, I., Kawabata, A., Kawachi, M., Kato, M. 2017 Fatty acid composition profiles of 235 strains of three microalgal divisions within the NIES Microbial Culture Collection. Microb. Resour. Syst., 33, 19-29. キーワード: Cryptophyta; docosahexaenoic acid; eicosapentaenoic acid; fatty acid; Haptophyta; Heterokontophyta; microalgae Strain(s): 1, 8, 9, 14, 15, 17, 71, 115, 145, 223, 225, 233, 234, 265, 274, 275, 276, 277, 278, 279, 280, 281, 282, 284, 293, 323, 324, 330, 333, 344, 345, 346, 347, 348, 350, 353, 372, 377, 388, 391, 395, 407, 408, 409, 413, 414, 417, 461, 462, 466, 487, 534, 548, 553, 556, 557, 558, 559, 560, 562, 587, 588, 589, 590, 603, 605, 621, 622, 623, 695, 696, 697, 698, 699, 700, 701, 702, 703, 704, 705, 706, 707, 708, 709, 710, 711, 712, 713, 714, 715, 716, 741, 765, 766, 767, 805, 837, 997, 1001, 1002, 1003, 1004, 1005, 1006, 1007, 1009, 1011, 1016, 1017, 1044, 1045, 1046, 1047, 1302, 1303, 1324, 1327, 1330, 1339, 1340, 1349, 1353, 1370, 1375, 1376, 1379, 1383, 1384, 1385, 1386, 1387, 1391, 1392, 1393, 1395, 1398, 1399, 1400, 1401, 1699, 1700, 1730, 1813, 1815, 1816, 1826, 1827, 1831, 1862, 1863, 1864, 1865, 1874, 1963, 1964, 1965, 2142, 2143, 2144, 2145, 2147, 2148, 2300, 2331, 2332, 2351, 2363, 2364, 2365, 2369, 2370, 2376, 2506, 2533, 2534, 2535, 2536, 2537, 2590, 2633, 2668, 2689, 2690, 2691, 2693, 2694, 2696, 2697, 2716, 2717, 2718, 2720, 2722, 2723, 2725, 2726, 2729, 2730, 2731, 2732, 2770, 2771, 2772, 2773, 2839, 2840, 2841, 2842, 2843, 2844, 2859, 2872, 2878, 2890, 2899 Iwai, M., Hori, K., Sasaki-Sekimoto, Y., Shimojima, M., Ohta, H. 2015 Manipulation of oil synthesis in Nannochloropsis strain NIES-2145 with a phosphorus starvation–inducible promoter from Chlamydomonas reinhardtii. Front. Microbiol., 6, 912 (article ID). キーワード: algae; Nannochloropsis; phosphorus starvation; inducible promoter; triacylglycerol Strain(s): 2145 PubMed: 26441858 DOI: 10.3389/fmicb.2015.00912 Nakanishi, K., Deuchi, K., Kuwano, K. 2012 Cryopreservation of four valuable strains of microalgae, including viability and characteristics during 15 years of cryostorage. J. Appl. Phycol., 24, 1381-1385. キーワード: Cryopreservation; Cryoprotectant; Chlorella vulgaris; Nannochloropsis oculata; Tetraselmis tetrathele; Chlorophyll Strain(s): 2145, 2170 DOI: 10.1007/s10811-012-9790-8 Sharifah, E. N., Eguchi, M. 2011 The phytoplankton Nannochloropsis oculata enhances the ability ofRoseobacter clade bacteria to inhibit the growth of Vibrio anguillarum. PLoS One, 6, e26756 (article ID). Strain(s): 2145 PubMed: 22053210 DOI: 10.1371/journal.pone.0026756

文献

Pollner, E., Farré, E. M., Bennlng, C. 2018 Advanced genetic tools enable synthetic biology in the oleaginous microalgae Nannochloropsis sp. Plant Cell Reports, 37, 1383-1399.
キーワード: Nannochloropsis; Algal biotechnology; Marker-free engineering; Gene stacking; Synthetic biology; Episomes
Strain(s): 2145, 2146
PubMed: 29511798
DOI: 10.1007/s00299-018-2270-0

Shimakawa, G., Murakami, A., Niwa, K., Matsuda, Y., Wada, A., Miyake, C. 2018 Comparative analysis of strategies to prepare electron sinks in aquatic photoautotrophs. Photosynth. Res.,
キーワード: Reactive oxygen species; P700 oxidation; Photosystem I; Seaweeds
Strain(s): 705, 1959, 2138, 2145, 2147
PubMed: 29845382
DOI: 10.1007/s11120-018-0522-z

Nobusawa, T., Hori, K., Mori, H., Kurokawa, K., Ohta, H. 2017 Differently localized lysophosphatidic acid acyltransferasescrucial for triacylglycerol biosynthesis in the oleaginous alga Nannochloropsis. Plant J., 90, 547–559.
キーワード: Nannochloropsis; triacylglycerol (TAG); biofuels; lysophosphatidic acid acyltransferase (LPAT); lipid droplets
Strain(s): 2145
PubMed: 28218992
DOI: 10.1111/tpj.13512

Mitani, E., Nakayama, F., Matsuwaki, I., Ichi, I., Kawabata, A., Kawachi, M., Kato, M. 2017 Fatty acid composition profiles of 235 strains of three microalgal divisions within the NIES Microbial Culture Collection. Microb. Resour. Syst., 33, 19-29.
キーワード: Cryptophyta; docosahexaenoic acid; eicosapentaenoic acid; fatty acid; Haptophyta; Heterokontophyta; microalgae
Strain(s): 1, 8, 9, 14, 15, 17, 71, 115, 145, 223, 225, 233, 234, 265, 274, 275, 276, 277, 278, 279, 280, 281, 282, 284, 293, 323, 324, 330, 333, 344, 345, 346, 347, 348, 350, 353, 372, 377, 388, 391, 395, 407, 408, 409, 413, 414, 417, 461, 462, 466, 487, 534, 548, 553, 556, 557, 558, 559, 560, 562, 587, 588, 589, 590, 603, 605, 621, 622, 623, 695, 696, 697, 698, 699, 700, 701, 702, 703, 704, 705, 706, 707, 708, 709, 710, 711, 712, 713, 714, 715, 716, 741, 765, 766, 767, 805, 837, 997, 1001, 1002, 1003, 1004, 1005, 1006, 1007, 1009, 1011, 1016, 1017, 1044, 1045, 1046, 1047, 1302, 1303, 1324, 1327, 1330, 1339, 1340, 1349, 1353, 1370, 1375, 1376, 1379, 1383, 1384, 1385, 1386, 1387, 1391, 1392, 1393, 1395, 1398, 1399, 1400, 1401, 1699, 1700, 1730, 1813, 1815, 1816, 1826, 1827, 1831, 1862, 1863, 1864, 1865, 1874, 1963, 1964, 1965, 2142, 2143, 2144, 2145, 2147, 2148, 2300, 2331, 2332, 2351, 2363, 2364, 2365, 2369, 2370, 2376, 2506, 2533, 2534, 2535, 2536, 2537, 2590, 2633, 2668, 2689, 2690, 2691, 2693, 2694, 2696, 2697, 2716, 2717, 2718, 2720, 2722, 2723, 2725, 2726, 2729, 2730, 2731, 2732, 2770, 2771, 2772, 2773, 2839, 2840, 2841, 2842, 2843, 2844, 2859, 2872, 2878, 2890, 2899

Iwai, M., Hori, K., Sasaki-Sekimoto, Y., Shimojima, M., Ohta, H. 2015 Manipulation of oil synthesis in Nannochloropsis strain NIES-2145 with a phosphorus starvation–inducible promoter from Chlamydomonas reinhardtii. Front. Microbiol., 6, 912 (article ID).
キーワード: algae; Nannochloropsis; phosphorus starvation; inducible promoter; triacylglycerol
Strain(s): 2145
PubMed: 26441858
DOI: 10.3389/fmicb.2015.00912

Nakanishi, K., Deuchi, K., Kuwano, K. 2012 Cryopreservation of four valuable strains of microalgae, including viability and characteristics during 15 years of cryostorage. J. Appl. Phycol., 24, 1381-1385.
キーワード: Cryopreservation; Cryoprotectant; Chlorella vulgaris; Nannochloropsis oculata; Tetraselmis tetrathele; Chlorophyll
Strain(s): 2145, 2170
DOI: 10.1007/s10811-012-9790-8

Sharifah, E. N., Eguchi, M. 2011 The phytoplankton Nannochloropsis oculata enhances the ability ofRoseobacter clade bacteria to inhibit the growth of Vibrio anguillarum. PLoS One, 6, e26756 (article ID).
Strain(s): 2145
PubMed: 22053210
DOI: 10.1371/journal.pone.0026756

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