Strain number NIES-543  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Volvox kirkiorum H.Nozaki, H.Kawai-Toyooka & N.Isaka  
Synonym  
Former name Volvox prolificus Iyengar  
Common name Green alga ; Volvocine alga  
Locality (Date of collection) Japan  
Latitude / Longitude  
Habitat (Isolation source) Freshwater  
History < Ogasawara, Yoshikazu  
Isolator (Date of isolation) Ogasawara, Yoshikazu  
Identified by Isaka, Nanako (Reidentify)  
State of strain Subculture; Unialgal; Clonal; Axenic[2018 Feb]  
Culture condition
(Preculture condition)
Medium:  VT  
Temperature:  23 C
Light intensity:  21-29 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  2 M  
Gene information ITS1-5.8S-ITS2 ( AB663327 ) , rbcL ( AB663325 ) , psbC ( AB663326 )  
Cell size (min - max)  
Organization Colonial; Flagellate 
Characteristics Phototaxis  
Other strain no. Other strain no. : V-sp  
Remarks Fragile species to transportation stresses; Read and agree "How to order 4.1"; Axenic 
Movie  
Reference
Hanschen, E. R., Herron, M. D., Wiens, J. J., Nozaki, H., Michod, R. E. 2018 Repeated evolution and reversibilityof self-fertilization in the volvocinegreen algae. Evolution, 72, 386-398.
Keywords: Haploid; phylogenetics; self-fertilization; sex; sexual reproduction; volvocine green algae
Strain(s): 28941845645846054154354454556456656856957157257457858265373285185685787287587687787888088288389189210381362171117132457256927383382338837803782 
PubMed: 29134623
DOI: 10.1111/evo.13394

Hanschen, E. R., Herron, M. D., Wiens, J. J., Nozaki, H., Michod, R. E. 2018 Multicellularity drives the evolution of sexual traits. Am. Nat., 192, E93-E10.
Keywords: sex; volvocine green algae; multicellularity; ancestral state; reconstruction; anisogamy; sexual dimorphism
Strain(s): 28941842145645846052254154354454556456656856957157257457858265373275185185685787287587687787888088288389189210381362171117132457256927383382338837803782 
PubMed: 30125231
DOI: 10.1086/698301

Hanschen, E. R., Davison, D. R., Ferris, P. J., Michod, R. E. 2018 On the rediscovery of Volvox perglobator (Volvocales, Chlorophyceae) and the evolution of outcrossing from self-fertilization. Evol. Ecol. Res., 19, 299-318.
Keywords: homothallism; phylogenetics; reversion; self-fertilization; sexual reproduction; volvocine green algae
Strain(s): 28942145645846047452254154354454556456656856957157257457858265373275185185687287687787888088210381122114011481156115713621708171117131859245725692738338233883780378239864145425842594260 

Isaka, N., Kawai-Toyooka, H., Matsuzaki, R., Nakada, T., Nozaki, H. 2012 Description of two new monoecious species of Volvox sect. Volvox (Volvocaceae, Chlorophyceae), based on comparative morphology and molecular phylogeny of cultured material. J. Phycol., 48, 759-767.
Keywords: Chlorophyceae; cultures; molecular phylogeny; morphology; taxonomy; Volvocaceae; Volvox ferrisii sp. nov.; Volvox kirkiorum sp. nov.; Volvox sect. Volvox
Strain(s): 54327362737273827392740 
PubMed: 27011093
DOI: 10.1111/j.1529-8817.2012.01142.x

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 192122232425262728293031323334353637383940414445465051525354555658596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123125127128129130131132133134135137138139144147151152153154155156157158159160162163164165166167168170171172173174175176177178179180181182183185186187188189191192193194197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268287288289290294295297298299300301302303305306308309310312313329334337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

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