Strain number NIES-173  
Phylum Streptophyta  
Class Charophyceae  
Scientific name Closterium moniliferum Ehrenberg ex Ralfs var. moniliferum  
Synonym Lunulina monilifera Bory 1824  
Former name  
Common name Desmid  
Locality (Date of collection) Kitamoto Saitama Japan (1969-01-**)  
Latitude / Longitude  
Habitat (Isolation source) Freshwater (Paddy soil)  
History < IAM (1983);  
Isolator (Date of isolation) Ichimura, Terunobu (1969-03-**)  
Identified by Ichimura, Terunobu  
State of strain Subculture; Unialgal; Clonal; Axenic[2017 Dec]  
Culture condition
(Preculture condition)
Medium:  C  
Temperature:  20 (20) C
Light intensity:  4-8 (13-21) µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  3 M (1 M)  
Gene information 5.8S-ITS2-28S ( AY148950 )  
Cell size (min - max) 30 - 250 μm  
Organization Unicellular 
Characteristics Homothallic  
Other strain no. Other collection strain no. : IAM C-432 (=C-573)
Other strain no. : S-1-22  
Remarks Axenic 
Movie  
Reference
Denboh, T., Ichimura, T., Hendrayanti, D., Coleman, A. W. 2003 Closterium moniliferum-ehrenbergii (Charophyceae, Chlorophyta) species complex viewed from the 1506 Group I intron and ITS2 of nuclear rDNA. J. Phycol., 39, 960-977.
Keywords: biological species; Closterium; group I introns; ITS2; mating group; molecular phylogeny; speciation
Strain(s): 172173174182183228229 
DOI: 10.1046/j.1529-8817.2003.02145.x

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19212223242526272829303132333435363738394041444546505152535455565758596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123124125127128129130131132133134135137138139144147148150151152153154155156157158159160162163164165166167168169170171172173174175176177178179180181182183185186187188189191192193194195197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268271287288289290294295297298299300301302303305306308309310311312313329334336337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

Ichimura, T., Itoh, T. 1977 17. Preservation methods of microalgae (I) [17. Bisaisôrui no hozonhô (I)]. In Preservation methods of microorganisms [Biseibutsu Hozonhô], Ed. by Nei, T., University of Tokyo Press, Tokyo, p. 355-373 (in Japanese without English title).
Strain(s): 19212223242728303132333436373839404144454647484950122123127131144152173175179180181182183185198200203206217226227241266267268271295309310334338379436448450455527 

Ichimura, T. 1973 Culture of microalgae (6). Iden, 27, 97-101 (in Japanese).
Strain(s): 66173175179180181183198262338450 

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