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株番号 NIES-483  
門名 Chlorophyta  
綱名 Nephroselmidophyceae 
学名 Nephroselmis olivacea Stein  
シノニム  
以前使われていた種名  
一般名  
地名(採集年月) 日本 茨城県 土浦市 (1986-02-**)  
緯度/経度 36.081096 / 140.16337 
生息環境(分離源) 淡水 (土)  
分離後の履歴 ← 須田彰一郎  
分離者(分離年月) 須田彰一郎 (1986-05-09)  
同定者 須田彰一郎  
株の状態 継代培養; 無菌[2014 Jan]; 単藻; クローン  
培養条件
(前培養条件)
培地名:  AF-6  
温度: 20 ℃
光強度: 10-15 µmol photons/m2/sec,  明暗周期: 10L:14D
継代培養周期: 2 M  
遺伝子情報 18S rRNA ( FN562436 ) , 18S-ITS1-5.8S-ITS2-28S ( HE610146 ) , 16S-ITS1-tRNA-Ile-tRNA-Ala-23S ( FN563086 ) , beta-tubulin ( AB491622 )  
特性 雌雄異株 ; 交配型 (+)  
サイズ (min - max) - 17 μm  
体制 単細胞性; 鞭毛性 
その他の株番号 その他の株番号:S-N-2-1  
注意事項 無菌  
動画  
文献
Marin, B. 2012 Nested in the Chlorellales or independent class? Phylogeny and classification of the Pedinophyceae (Viridiplantae) revealed by molecular phylogenetic analyses of complete nuclear and plastid-encoded rRNA operons. Protist, 163, 778-805.
キーワード: Marsupiomonadales; Marsupiomonas; Pedinomonas; Pedinophyceae; Resultor; Resultomonas
Strain(s): 252483 
PubMed: 22192529
DOI: 10.1016/j.protis.2011.11.004

Marin, B., Melkonian, M. 2010 Molecular phylogeny and classification of the Mamiellophyceae class. nov. (Chlorophyta) based on sequence comparisons of the nuclear- and plastid-encoded rRNA operons. Protist, 161, 304-336.
キーワード: prasinophytes; SSU rDNA; LSU rDNA; ITS2; systematics; freshwater; Monomastigales; Dolichomastigales; Mamiellales; Monomastigaceae; Dolichomastigaceae; Crustomastigaceae; Bathycoccaceae
Strain(s): 252255360483 
PubMed: 20005168
DOI: 10.1016/j.protis.2009.10.002

Takahashi, F., Okabe, Y., Nakada, T., Sekimoto, H., Ito, M., Kataoka, H. & Nozaki, H. 2007 Origins of the secondary plastids of Euglenophyta and Chlorarachniophyta as revealed by an analysis of the plastid-targeting, nuclear-encoded gene psbO. J. Phycol., 43, 1302-1309.
キーワード: alanine-X-alanine motif; Chlorarachniophyta; Euglenophyta; phylogeny; plastids; psbO; secondary endosymbiosis; transit peptide; thylakoid-targeting domain
Strain(s): 67203254381483533624656 
DOI: 10.1111/j.1529-8817.2007.00411.x

Nowack, E. C. M., Podola, B., Melkonian, M. 2005 The 96-well twin-layer system: a novel approach in the cultivation of microalgae. Protist, 156, 239-251.
キーワード: cultivation; culture collections; cell immobilisation; microalgae; microtiter plates; filter plates
Strain(s): 255483485 
PubMed: 16171190
DOI: 10.1016/j.protis.2005.04.003

Yoshii, Y., Takaichi, S., Maoka, T., Suda, S., Sekiguchi, H., Nakayama, T., Inouye, I. 2005 Variation of siphonaxanthin series among the genus Nephroselmis (Prasinophyceae, Chlorophyta), including a novel primary methoxy carotenoid. J. Phycol., 41, 827-834.
キーワード: carotenoids; methylation; Nephroselmis; photosynthetic antenna system; Prasinophyceae; siphonaxanthin series
Strain(s): 2524834869349352309 
DOI: 10.1111/j.1529-8817.2005.00103.x

Hamana, K., Aizaki T., Arai, E., Saito, A., Uchikata, K., Ohnishi, H. 2004 Distribution of norspermidine s a cellular polyamine within micro green algae including non-photosynthetic achlorophyllous Polytoma, Polytomella, Prototheca and Helicosporidium. J. Gen. Appl. Microbiol., 50, 289-295.
キーワード: Chlorophyta; green alga; Helicosporidium; norspermidine; polyamine; Polytoma; Polytomella; Prototheca
Strain(s): 18251254255296329483486536 

Suda, S., Watanabe, M. M., Inouye, I. 2004 Electron microscopy of sexual reproduction in Nephroselmis olivacea (Prasinophyceae, Chlorophyta). Phycol. Res., 52, 273-283.
キーワード: electron microscopy; endoplasmic reticulum; germination; Nephroselmis olivacea; Prasinophyceae; sexual reproduction; zygote formation; zygote wall
Strain(s): 483485 
DOI: 10.1111/j.1440-183.2004.00346.x

Marin, B., Melkonian, M. 1994 Flagellar hairs in prasinophytes (Chlorophyta): ultrastructure and distribution on the flagellar surface. J. Phycol., 30, 659-678.
キーワード: flagellar hairs; Mamiella; Mantoniella; Nephroselmis; Prasinophyceae; Pseudoscourfieldia; Pterosperma; Pyramimonas; Scherffelia; ultrastructure
Strain(s): 221483485 
DOI: 10.1111/j.0022-3646.1994.00659.x

Suda, S., Watanabe, M. M., Inouye, I. 1989 Evidence for sexual reproduction in the primitive green alga Nephroselmis olivacea (Prasinophyceae). J. Phycol., 25, 596-600.
Strain(s): 483485 
DOI: 10.1111/j.1529-8817.1989.tb00266.x

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