Strain number NIES-2146  
Phylum Heterokontophyta  
Class Eustigmatophyceae  
Scientific name Nannochloropsis oculata (Droop) Hibberd  
Synonym  
Former name  
Common name  
Locality (Date of collection) Institute of Fisheries Nagasaki Nagasaki Japan  
Latitude / Longitude  
Habitat (Isolation source) Marine  
History < IAM (2007) < Maruyama, Ko (around 1986)  
Isolator (Date of isolation) Maruyama, Ko (1986-**-**)  
Identified by Maruyama, Ko, et al.  
State of strain Subculture; Unialgal; Clonal; Axenic[2018 Feb]  
Culture condition
(Preculture condition)
Medium:  ESM (agar)  
Temperature:  20 C
Light intensity:  5-9 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  4 M  
Gene information  
Cell size (min - max) 3 - 8 μm  
Organization Unicellular 
Characteristics Live food (Wullur et al. 2011)  
Other strain no. Other collection strain no. : IAM ST-6
 
Remarks Axenic 
Movie  
Reference
Pollner, E., Farré, E. M., Bennlng, C. 2018 Advanced genetic tools enable synthetic biology in the oleaginous microalgae Nannochloropsis sp. Plant Cell Reports, 37, 1383-1399.
Keywords: Nannochloropsis; Algal biotechnology; Marker-free engineering; Gene stacking; Synthetic biology; Episomes
Strain(s): 21452146 
PubMed: 29511798
DOI: 10.1007/s00299-018-2270-0

Shih, C., Chen, H., Lee, H., Tsai, H. 2015 Purple chromoprotein gene serves as a new selection marker for transgenesis of the microalga Nannochloropsis oculata. PLoS One, 10, e0120780 (article ID).
Strain(s): 2146 
PubMed: 25793255
DOI: 10.1371/journal.pone.0120780

Fukuda, S., Iwamoto, K., Atsumi, M., Yokoyama, A., Nakayama, T., Ishida, K-I., Inouye, I., Shiraiwa, Y. 2014 Global searches for microalgae and aquatic plants that can eliminate radioactive cesium, iodine and strontium from the radio-polluted aquatic environment: a bioremediation strategy. J. Plant Res., 127, 79-89.
Keywords: Algal phytoremediation; Bioaccumulation; Radiopollution; Radionuclide elimination; Radioactive cesium; The Fukushima 1 Nuclear Power Plant accident
Strain(s): 243671155160203233246329333391405417440487503529531538548553587597727805843859931995100310121015101710311044104512591326133113771382138814111435143914401441144214581728182618311833184018461862186519562131214421462147215021752176218522682325234123522377241124122437285428552856285828592860 
PubMed: 24346654
DOI: 10.1007/s10265-013-0596-9

Wullur, S., Sakakura, Y., Hagiwara, A. 2011 Application of the minute monogonont rotifer Proales similis de Beauchamp in larval rearing of seven-band grouper epinephelus septemfasciatus. Aquaculture, 315, 355-360.
Keywords: Rotifer; Proales similis; Brachionus rotundiformis; Live food; Fatty acid; Epinephelus septemfasciatus
Strain(s): 2146 
DOI: 10.1016/j.aquaculture.2011.02.025

Maruyama, I., Nakamura, T., Matubayashi, T., Ando, Y., Maeda, T. 1986 Identification of the alga known as "marine Chlorella" as a member of the Eustigmatophyceae. Jpn. J. Phycol. (Sôrui), 34, 319-325.
Strain(s): 2146 

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