Strain number NIES-483  
Phylum Chlorophyta  
Class Nephroselmidophyceae  
Scientific name Nephroselmis olivacea Stein  
Synonym -----  
Former name  
Common name  
Locality (Date of collection) Tsuchiura Ibaraki Japan (1986-02-**)  
Latitude / Longitude 36.081096 / 140.16337 
Habitat (Isolation source) Freshwater (Pond sediment)  
History < Suda, Shoichiro  
Isolator (Date of isolation) Suda, Shoichiro (1986-05-09)  
Identified by Suda, Shoichiro  
State of strain Subculture; Unialgal; Clonal; Axenic[2014 Jan]  
Culture condition
(Preculture condition)
Medium:  AF-6  
Temperature:  20 C
Light intensity:  10-15 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  2 M  
Gene information 18S rRNA ( FN562436 ) , 18S-ITS1-5.8S-ITS2-28S ( HE610146 ) , 16S-ITS1-tRNA-Ile-tRNA-Ala-23S ( FN563086 ) , beta-tubulin ( AB491622 )  
Cell size (min - max) - 17 μm  
Organization Unicellular; Flagellate 
Characteristics Heterothallic ; Mating type (+)  
Other strain no. Other strain no. : S-N-2-1  

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