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Strain number NIES-483  
Phylum Chlorophyta  
Class Nephroselmidophyceae  
Scientific name Nephroselmis olivacea Stein  
Synonym -----  
Former name  
Common name  
Locality (Date of collection) Tsuchiura Ibaraki Japan (1986-02-**)  
Latitude / Longitude 36.081096 / 140.16337 
Habitat (Isolation source) Freshwater (Pond sediment)  
History < Suda, Shoichiro  
Isolator (Date of isolation) Suda, Shoichiro (1986-05-09)  
Identified by Suda, Shoichiro  
State of strain Subculture; Unialgal; Clonal; Axenic[2014 Jan]  
Culture condition
(Preculture condition)
Medium:  AF-6  
Temperature:  20 C
Light intensity:  10-15 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  45 D  
Gene information 18S rRNA ( FN562436 ) , 18S-ITS1-5.8S-ITS2-28S ( HE610146 ) , 16S-ITS1-tRNA-Ile-tRNA-Ala-23S ( FN563086 ) , beta-tubulin ( AB491622 )  
Cell size (min - max) - 17 μm  
Organization Unicellular; Flagellate 
Characteristics Heterothallic ; Mating type (+)  
Other strain no. Other strain no. : S-N-2-1  
Remarks  
Movie  
Reference
Marin, B. 2012 Nested in the Chlorellales or independent class? Phylogeny and classification of the Pedinophyceae (Viridiplantae) revealed by molecular phylogenetic analyses of complete nuclear and plastid-encoded rRNA operons. Protist, 163, 778-805.
Keywords: Marsupiomonadales; Marsupiomonas; Pedinomonas; Pedinophyceae; Resultor; Resultomonas
Strain(s): 252483 
PubMed: 22192529
DOI: 10.1016/j.protis.2011.11.004

Marin, B., Melkonian, M. 2010 Molecular phylogeny and classification of the Mamiellophyceae class. nov. (Chlorophyta) based on sequence comparisons of the nuclear- and plastid-encoded rRNA operons. Protist, 161, 304-336.
Keywords: prasinophytes; SSU rDNA; LSU rDNA; ITS2; systematics; freshwater; Monomastigales; Dolichomastigales; Mamiellales; Monomastigaceae; Dolichomastigaceae; Crustomastigaceae; Bathycoccaceae
Strain(s): 252255360483 
PubMed: 20005168
DOI: 10.1016/j.protis.2009.10.002

Takahashi, F., Okabe, Y., Nakada, T., Sekimoto, H., Ito, M., Kataoka, H. & Nozaki, H. 2007 Origins of the secondary plastids of Euglenophyta and Chlorarachniophyta as revealed by an analysis of the plastid-targeting, nuclear-encoded gene psbO. J. Phycol., 43, 1302-1309.
Keywords: alanine-X-alanine motif; Chlorarachniophyta; Euglenophyta; phylogeny; plastids; psbO; secondary endosymbiosis; transit peptide; thylakoid-targeting domain
Strain(s): 67203254381483533624656 

Nowack, E. C. M., Podola, B., Melkonian, M. 2005 The 96-well twin-layer system: a novel approach in the cultivation of microalgae. Protist, 156, 239-251.
Keywords: cultivation; culture collections; cell immobilisation; microalgae; microtiter plates; filter plates
Strain(s): 255483485 

Yoshii, Y., Takaichi, S., Maoka, T., Suda, S., Sekiguchi, H., Nakayama, T., Inouye, I. 2005 Variation of siphonaxanthin series among the genus Nephroselmis (Prasinophyceae, Chlorophyta), including a novel primary methoxy carotenoid. J. Phycol., 41, 827-834.
Keywords: carotenoids; methylation; Nephroselmis; photosynthetic antenna system; Prasinophyceae; siphonaxanthin series
Strain(s): 2524834869349352309 

Hamana, K., Aizaki T., Arai, E., Saito, A., Uchikata, K., Ohnishi, H. 2004 Distribution of norspermidine s a cellular polyamine within micro green algae including non-photosynthetic achlorophyllous Polytoma, Polytomella, Prototheca and Helicosporidium. J. Gen. Appl. Microbiol., 50, 289-295.
Keywords: Chlorophyta; green alga; Helicosporidium; norspermidine; polyamine; Polytoma; Polytomella; Prototheca
Strain(s): 18251254255296329483486536 

Suda, S., Watanabe, M. M., Inouye, I. 2004 Electron microscopy of sexual reproduction in Nephroselmis olivacea (Prasinophyceae, Chlorophyta). Phycol. Res., 52, 273-283.
Keywords: electron microscopy; endoplasmic reticulum; germination; Nephroselmis olivacea; Prasinophyceae; sexual reproduction; zygote formation; zygote wall
Strain(s): 483485 

Marin, B., Melkonian, M. 1994 Flagellar hairs in prasinophytes (Chlorophyta): ultrastructure and distribution on the flagellar surface. J. Phycol., 30, 659-678.
Keywords: flagellar hairs; Mamiella; Mantoniella; Nephroselmis; Prasinophyceae; Pseudoscourfieldia; Pterosperma; Pyramimonas; Scherffelia; ultrastructure
Strain(s): 221483485 

Suda, S., Watanabe, M. M., Inouye, I. 1989 Evidence for sexual reproduction in the primitive green alga Nephroselmis olivacea (Prasinophyceae). J. Phycol., 25, 596-600.
Strain(s): 483485 

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