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Strain number NIES-226  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Graesiella emersonii (Shihira & Krauss) Nozaki et al.  
Synonym Chlorella emersonii Shihira et Krauss; Chlorella fusca Shihira et Krauss var. vacuolata Shihira et Krauss; Graesiella vacuolata (Shihira et Krauss) Kalina et Puncochárová  
Former name Chlorella pyrenoidosa Chick  
Common name Green alga  
Locality (Date of collection) Japan (-----)  
Latitude / Longitude  
Habitat (Isolation source)  
History < IAM (1983);  
Isolator (Date of isolation) (-----)  
Identified by Nozaki, Hisayoshi et al. (Reidentify)  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2014 Feb]  
Culture condition
(Preculture condition)
Medium:  C (agar)  
Temperature:  20 (25) C
Light intensity:  4-10 (15-19) µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  3 M (14 D)  
Gene information  
Cell size (min - max) 5 - 9 μm  
Organization Unicellular 
Characteristics  
Other strain no. Other collection strain no. : IAM C-28
 
Remarks Cryopreserved 
Movie  
Reference
Hamasaki, A. 2017 Study of the optimal cultivation for micro-algae. 第12回バイオマス科学会議発表論文集, 27-28 (in Japanese with English summary).
Strain(s): 226 
DOI: 10.20550/jiebiomassronbun.12.0_27

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19212223242526272829303132333435363738394041444546505152535455565758596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123124125127128129130131132133134135137138139144147148150151152153154155156157158159160162163164165166167168169170171172173174175176177178179180181182183185186187188189191192193194195197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268271287288289290294295297298299300301302303305306308309310311312313329334336337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

Kuramoto, T., Iwamoto, K., Izumi, M., Kirihata, M., Yoshizako, F. 1999 Asymmetric reduction of ethyl 2-methyl 3-oxobutanoate by Chlorella. Biosci. Biotechnol. Biochem., 63, 598-601.
Keywords: Chlorella; asymmetric reduction; ethyl 2-methyl 3-oxobutanoate; (2S, 3S)-ethyl 2-methyl 3-hydroxybutanoate; (2S, 3R)-ethyl 2-methyl 3-hydroxybutanoate
Strain(s): 226227 

Nozaki, H., Katagiri, M., Nakagawa, M., Aizawa, K., Watanabe, M. M. 1995 Taxonomic re-examination of the two strains labeled Chlorella in the Microbial Culture Collection at the National Institute for Environmental Studies (NIES-Collection). Microbiol. Cult. Coll., 11, 11-18.
Keywords: Chlorella; Graesiella emersonii comb. nov.; green algae; morphology; taxonomy
Strain(s): 226227686687688689690 

Yoshizako, F., Ogino, M., Nishimura, A., Chubachi, M., Horii, T. 1995 Biotransformation of cyclic β-keto esters by Chlorella pyrenoidosa Chick. J. Ferment. Bioeng., 79, 141-145.
Keywords: Chlorella pyrenoidosa Chick; cyclic β-keto esters; biotransformation; alkoxycarbolyl group elimination; carbonyl group reduction
Strain(s): 226 

Yoshizako, F., Nishimura, A., Chubachi, M. 1994 Identification of algal transformation products from alicyclic ketones. J. Ferment. Bioeng., 77, 144-147.
Strain(s): 226 

Watanabe, Y., Ohmura, N., Saiki, H. 1992 Microbial CO2 fixation. 2. Isolation and determination of cultural characteristics of Chlorella strains which function under CO2 enriched atmosphere. CRIEPI Kenkyû-Hôkoku, U92014, 1-21 (in Japanese with English summary).
Keywords: climate change; CO2; CO2 fixation; microalgae; Chlorella
Strain(s): 27459294122158204226227 

Yoshizako, F., Nishimura, A., Chubachi, M. 1992 Microbial reduction of cyclohexanone by Chlorella pyrenoidosa Chick. J. Ferment. Bioeng., 74, 395-397.
Strain(s): 226 

Yoshizako, F., Nishimura, A., Chubachi, M., Horii, T., Ueno, T. 1991 Bioconversion of cyclohexaneacetic acid to monohydroxycyclo-hexaneacetic acids by Chlorella pyrenoidosa Chick. J. Ferment. Bioeng., 72, 343-346.
Strain(s): 226227 

Maruyama, I., Ando, Y., Maeda, T., Hirayama, K. 1989 Uptake of vitamin B12 by various strains of unicellular algae Chlorella. Nippon Suisan Gakkaishi, 55, 1785-1790.
Strain(s): 48226687215721692175 

Takeda, H. 1988 Classification of Chlorella strains by cell wall sugar composition. Phytochemistry, 27, 3823-3826.
Keywords: Chlorella ellipsoidea; C. fusca; C. kessleri; C. saccharophila; C. sorokiniana; C. vulgaris; Chlorophyta; anisotropy; cell wall sugar composition; chemotaxonomy; Ruthenium Red staining
Strain(s): 226215021602170 

Hirokawa, T., Takeda, H. 1980 Automatic analysing system for the liquid chromatography. J. Gen. Edu. Dept., Niigata Univ., 10, 97-106.
Strain(s): 2262170 

Ichimura, T., Itoh, T. 1977 17. Preservation methods of microalgae (I) [17. Bisaisôrui no hozonhô (I)]. In Preservation methods of microorganisms [Biseibutsu Hozonhô], Ed. by Nei, T., University of Tokyo Press, Tokyo, p. 355-373 (in Japanese without English title).
Strain(s): 19212223242728303132333436373839404144454647484950122123127131144152173175179180181182183185198200203206217226227241266267268271295309310334338379436448450455527 

Maruyama, K. 1977 Classification of Chlorella strain by infrared absorption spectra of cell samples. Bot. Mag. Tokyo, 90, 67-77.
Strain(s): 2262276856872151216321702175 

Maruyama, K. 1977 Classification of Chlorella strains by cell appearance and group sera. Bot. Mag. Tokyo, 90, 57-66.
Strain(s): 2262276852151216321702175 

Mihara, S., Hase, E. 1976 A short note on the effects of 6-methyl purine on the revolution of the cell cycle of Chlorella pyrenoidosa in synchronous culture. Plant Cell Physiol., 17, 403-406.
Strain(s): 226 

Naohara, T., Ishikawa, H. 1975 Assay of the activity of herbicides using Chlorella. Zassô-Kenkyû, 20, 82-86 (in Japanese with English summary).
Strain(s): 226 

Watanabe, A. 1960 List of algal strains in collection at the Institute of Applied Microbiology, University of Tokyo. J. Gen. Appl. Microbiol., 6, 283-292.
Strain(s): 192122232425262829374749226227 

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