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株番号 NIES-446  
門名 Chlorophyta  
綱名 Chlorophyceae 
学名 Chlamydomonas tetragama (Bohlin) Ettl  
シノニム  
以前使われていた種名 Chlorogonium metamorphum Skuja  
一般名 緑藻 ; クラミドモナス  
地名(採集年月) 日本 茨城県 土浦市 (1985-04-13)  
緯度/経度 36.081096 / 140.16337 
生息環境(分離源) 淡水 (水)  
分離後の履歴 ← 須田彰一郎  
分離者(分離年月) 須田彰一郎 (1985-04-13)  
同定者 須田彰一郎  
株の状態 継代培養; 無菌[2014 Jan]; 単藻; クローン  
培養条件
(前培養条件)
培地名:  C  
温度: 20 ℃
光強度: 10-15 µmol photons/m2/sec,  明暗周期: 10L:14D
継代培養周期: 2 M  
遺伝子情報 atpB ( AB084319 ) , psaB ( AB084357 ) , rbcL ( AJ001880 ) , 18S rRNA ( AB007370 )  
特性 走光性 ; Authentic株, ネオタイプ  
サイズ (min - max) - 19 μm  
体制 単細胞性; 鞭毛性 
その他の株番号 その他の株番号:413D4-4  
注意事項  
動画  
文献
Munakata, H., Nakada, T., Nakahigashi, K., Nozaki, H., Tomita, M. 2016 Phylogenetic position and molecular chronology of a colonial green flagellate, Stephanosphaera pluvialis (Volvocales, Chlorophyceae), among unicellular algae. J. Eucaryot. Microbiol., 63, 340-348.
キーワード: Balticola; coloniality; divergence time; evolution; molecular phylogeny
Strain(s): 123144421428432439446567569571656861887968104822932564 
DOI: 10.1111/jeu.12283

Luo, W., Kotut, K., Krienits, L. 2013 Hidden diversity of eukaryotic plankton in the soda lake Nakuru, Kenya, during a phase of low salinity revealed by a SSU rRNA gene clone library. Hydrobiologia, 702, 95-103.
キーワード: Chlorophyta; clone library; lake Nakuru; lesser flamingo; phytoflagellates; Soda lakes; SSU rRNA
Strain(s): 4461360 
DOI: 10.1007/s10750-012-1310-y

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
キーワード: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19212223242526272829303132333435363738394041444546505152535455565758596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123124125127128129130131132133134135137138139144147148150151152153154155156157158159160162163164165166167168169170171172173174175176177178179180181182183185186187188189191192193194195197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268271287288289290294295297298299300301302303305306308309310311312313329334336337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

Nozaki, H., Onishi, K., Morita, E. 2002 Differences in pyrenoid morphology are correlated with differences in the rbcL genes of members of the Chloromonas lineage (Volvocales, Chlorophyceae). J. Mol. Evol., 55, 414-430.
キーワード: ATP synthase β-subunit (atpB) gene; Chloromonas; gene evolution; large subunit of Rubisco (rbcL) gene; morphological evolution; P700 chlorophyll a-apoprotein A2 (psaB) gene; pyrenoid; Volvocales
Strain(s): 96123144213418421425428432439446447456474522564566567569571574582653732851858861875882883968 
PubMed: 12355262
DOI: 10.1007/s00239-002-2338-9

Hepperle, D., Nozaki, H., Hohenberger, S., Huss, V. A. R., Morita, E., Krienitz, L. 1998 Phylogenetic position of the Phacotaceae within the Chlamydophyceae as revealed by analysis of 18S rDNA and rbcL sequences. J. Mol. Evol., 47, 420-430.
キーワード: Phacotaceae; Volvocales; 18S rDNA phylogeny; lorica-bearing flagellates; green algae
Strain(s): 446858859861862 
PubMed: 9767687
DOI: 10.1007/PL00006399

Nozaki, H., Ohta, N., Morita, E., Watanabe, M. M. 1998 Toward a natural system of species in Chlorogonium (Volvocales, Chlorophyta): a combined analysis of morphological and rbcL gene sequence data. J. Phycol., 34, 1024-1037.
キーワード: Chlorogonium; Chlorogonium kasakii sp. nov.; Chlorophyta; molecular phylogeny; monophyletic species; morphology; natural systematics; rbcL gene; species taxonomy; Volvocales
Strain(s): 123439446692742743744745746747748749750751752753754755756757758759760761 
DOI: 10.1046/j.1529-8817.1998.341024.x

Uchida, H., Kitae, K., Tomizawa, K., Yokota, A. 1998 Comparison of the nucleotide sequence and secondary structure of the 5.8S ribosomal RNA gene of Chlamydomonas tetragama with those of green algae. DNA Seq., 8, 403-408.
キーワード: 5.8S ribosomal RNA gene; Chlamydomonas
Strain(s): 446 

Nozaki, H., Aizawa, K., Watanabe, M. M. 1996 Re-examination of two NIES strains labeled Chlorogonium metamorphum (Volvocales, Chlorophyta) from Japan. Nova Hedwigia Beiheft, 112, 483-490.
キーワード: Chlorogonium; Chlorophyta; morphology; neotype strain; species identification; Volvocales
Strain(s): 123446 

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