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Strain number NIES-212  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Pediastrum duplex Meyen  
Synonym -----  
Former name  
Common name Green alga  
Locality (Date of collection) Lake Kawaguchi Yamanashi Japan (1981-06-**)  
Latitude / Longitude 35.5130910551 / 138.748515637 
Habitat (Isolation source) Freshwater (Lake water)  
History < Watanabe, Michiko H.  
Isolator (Date of isolation) Watanabe, Michiko H. (1981-06-**)  
Identified by Watanabe, Michiko H.  
State of strain Cryopreservation; Unialgal; Clonal; Non-axenic  
Culture condition
(Preculture condition)
Medium:  C  
Temperature:  20 C
Light intensity:  8-15 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  2 M  
Gene information  
Cell size (min - max) - 17 μm  
Organization Colonial 
Characteristics  
Other strain no. Other strain no. : KW-P-1  
Remarks Cryopreserved 
Movie  
Reference
Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19212223242526272829303132333435363738394041444546505152535455565758596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123124125127128129130131132133134135137138139144147148150151152153154155156157158159160162163164165166167168169170171172173174175176177178179180181182183185186187188189191192193194195197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268271287288289290294295297298299300301302303305306308309310311312313329334336337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

Ueda, K., Nonaka, M. 1992 Division of chloroplasts in a green alga, Pediastrum duplex. Ann. Bot., 69, 113-118.
Keywords: cell wall; chloroplast division; freeze-substitution; intermediate fibres; Pediastrum duplex; transmission electron microscopy
Strain(s): 212 

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