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Strain number NIES-2142  
Phylum Heterokontophyta  
Class Chrysophyceae  
Scientific name Ochromonas danica Pringsheim  
Synonym  
Former name  
Common name  
Locality (Date of collection) near Everdrup Denmark  
Latitude / Longitude 55.203341 / 11.94647 
Habitat (Isolation source) Freshwater  
History < IAM (2007) < UTEX (1996);  
Isolator (Date of isolation) Pringsheim, E. G. (1954-**-**)  
Identified by Pringsheim, E. G.  
State of strain Subculture; Unialgal; Clonal; Axenic[2014 Feb]  
Culture condition
(Preculture condition)
Medium:  O (semi-solid)  
Temperature:  20 C
Light intensity:  13-16 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information ITS1-5.8S-ITS2 ( Y07976 ) , 16S rRNA ( X53183 ) , 28S rRNA ( KM590767 GU935636 Y07977 ) , gapC ( AJ512345 ) , gapC1 ( AJ512344 ) , psaA ( KM590836 ) , rbcL ( GU935657 ) , vppa ( AJ251725 ) , odbgp ( LC140879 )  
Cell size (min - max)  
Organization  
Characteristics Mixotrophic ; Phagotrophic  
Other strain no. Other collection strain no. : IAM CS-4 (=CS-2); ATCC 30004; CCAP 933/2; SAG 933-7; UTEX L 1298; CCAP 933/2B: CCMP 585
 
Remarks  
Movie  
Reference
Mitani, E., Nakayama, F., Matsuwaki, I., Ichi, I., Kawabata, A., Kawachi, M., Kato, M. 2017 Fatty acid composition profiles of 235 strains of three microalgal divisions within the NIES Microbial Culture Collection. Microb. Resour. Syst., 33, 19-29.
Keywords: Cryptophyta; docosahexaenoic acid; eicosapentaenoic acid; fatty acid; Haptophyta; Heterokontophyta; microalgae
Strain(s): 1891415177111514522322523323426527427527627727827928028128228429332332433033334434534634734835035337237738839139540740840941341441746146246648753454855355655755855956056258758858959060360562162262369569669769869970070170270370470570670770870971071171271371471571674176576676780583799710011002100310041005100610071009101110161017104410451046104713021303132413271330133913401349135313701375137613791383138413851386138713911392139313951398139914001401169917001730181318151816182618271831186218631864186518741963196419652142214321442145214721482300233123322351236323642365237025062533253425352536253725902633266826892690269126932694269626972716271727182720272227232725272627292730273127322770277127722773283928402841284228432844285928782890 

Yamamoto, Y., Kawashima, D., Hashizume, A., Hisamotsu, M., Isono, N. 2013 Purification and characterization of 1,3-β-D-glucan phosphorylase from Ochromonas danica. Biosci. Biotechnol. Biochem., 77, 130411-130416.
Keywords: 1,3-β-D-glucan phosphorylase; Ochromonas danica; laminarin; laminarioligosaccharides; 1,3-β-D-glucan
Strain(s): 2142 
DOI: 10.1271/bbb.130411

Kawahara, T., Kumaki, Y., Kamada, T., Ishii, T., Okino, T. 2008 Absolute configuration of chlorosulfolipids from the chrysophyta Ochromonas danica. J. Org. Chem., 74, 6016-6024.
Strain(s): 2142 

McKay, R. M., Gibbs, S. P. 1989 Immunocytochemical localization of ribulose 1,5-bisphosphate carboxylase/oxygenase in light-limited and light-saturated cells of Chlorella pyrenoidosa. Protoplasma, 149, 31-37.
Keywords: pyrenoid; ribulose 1,5-bisphosphate carboxylase/oxygenase; Chlorella pyrenoidosa; immunocytochemistry
Strain(s): 6872142 

Witt, D., Stackebrandt, E. 1988 Disproving the hypothesis of a common ancestry for the Ochromonas danica chrysoplast and Heliobacterium chlorum. Arch. Mikrobiol., 150, 244-248.
Strain(s): 2142 

Coleman, A. W. 1988 The autofluorescent flagellum: a new phylogenetic enigma. J. Phycol., 24, 118-120.
Keywords: autofluorescence; Chrysophyceae; flagellum; fluorescence; heterokont; Ochromonas; photoreceptor; Prymnesium
Strain(s): 21422144 

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