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Strain number NIES-127  
Phylum Streptophyta  
Class Charophyceae  
Scientific name Closterium acerosum Ehrenberg ex Ralfs  
Synonym -----  
Former name  
Common name Desmid  
Locality (Date of collection) Sapporo Hokkaido Japan (-----)  
Latitude / Longitude  
Habitat (Isolation source) Freshwater (Stream sediment)  
History < IAM (1983);  
Isolator (Date of isolation) Nishihama, Yuji (-----)  
Identified by Niiyama, Yuko  
State of strain Subculture; Unialgal; Clonal; Axenic[2013 Dec]  
Culture condition
(Preculture condition)
Medium:  C  
Temperature:  20 (20) C
Light intensity:  7-8 (13-21) µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  3 M (1 M)  
Gene information  
Cell size (min - max)  
Organization Unicellular 
Characteristics Homothallic  
Other strain no. Other collection strain no. : IAM C-435
Other strain no. : H-2-2  
Remarks  
Movie  
Reference
Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19212223242526272829303132333435363738394041444546505152535455565758596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123124125127128129130131132133134135137138139144147148150151152153154155156157158159160162163164165166167168169170171172173174175176177178179180181182183185186187188189191192193194195197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268271287288289290294295297298299300301302303305306308309310311312313329334336337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

Ichimura, T., Itoh, T. 1977 17. Preservation methods of microalgae (I) [17. Bisaisôrui no hozonhô (I)]. In Preservation methods of microorganisms [Biseibutsu Hozonhô], Ed. by Nei, T., University of Tokyo Press, Tokyo, p. 355-373 (in Japanese without English title).
Strain(s): 19212223242728303132333436373839404144454647484950122123127131144152173175179180181182183185198200203206217226227241266267268271295309310334338379436448450455527 

Ichimura, T. 1973 The life cycle and its control in some species of Closterium, with special reference to the biological species problems. Thesis D. Sci., University of Tokyo, 69 pp., 11 tables, 40 figs.
Strain(s): 666768124125127174175176177179180181182183185198202262337338450451 

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