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1 淡水産、陸生、温泉産、塩水産藻類用培地

AAF–6* Acid–CSi/5* AF–6* AF–6/2*
AFAC* Allen* BBM BG–11*
BG–11 (寒天) * C* C (寒天) * C+10% Seawater
C/6G CA* CAM* Carefoot*
CB* CB–V* CC CSi*
CSi + Cu (軟寒天) * CSi/5* CT* CYT*
DH + Fe (寒天) DY–V* HUT* HUT (軟寒天) *
M–11 MA* MAF–6* M–Allen*
M–Allen(+ U)* MBM M Chu No. 10 MDM*
MDM (寒天) * MG* MGM Modified acetate medium (mAC) *
Modified M–1 (mM–1) MW MW/5 N–Free (寒天) *
O (軟寒天) * P 35* Pro (寒天) * SOT*
SW TAP (寒天) * Tre (寒天) URO*
URO–H* URO–T VT* VTAC*
VTYT W

2 海産および汽水産藻類用培地

BESM* BESM 2* ESM* ESM (寒天) * ESM2* f/2 + NH4Cl* f/2*
IMK K* K/2* K/2ET* M–ASP7 MF mIMR* ( modified IMR )
MKM MNK* PRO–99* WESM*

3 淡水産藻類用無菌検査培地

B–I B–II B–III B–IV B–V YT

4 海産藻類用無菌検査培地

Bf/2 MM 23 STP*

5 微量金属、ビタミン混液、土壌浸出液

A2 Fe stock solution A2 trace element stock solution A5 solution Allen metals
C–Source Mix II D stock medium D trace mix DY trace metal solution
Fe ( as EDTA; 1:1 molar )* Fe solution f/2 metals* Hutner ’s trace elements
K metals K/2 metals mIMR trace metals* mM–1 Trace elements
mTYGM–9* P II metals P IV metals* PN metals
PRO–99 trace metals Soil extract Trace metal mix A5 + Co Vitamin mix S3
Waris EDTA Waris P-2

6 原生動物用培地

AF–6 + Wheat ESM + mTYGM–9 + Rice f/2 + mTYGM–9 + Rice f/2 + Wheat
Helio* LE PYG* SUY*
SUY 1/10* SUY 1/10 + mTYGM–9 + Rice SUY 1/10 + Wheat URO + Wheat
URO–H + Wheat UYTS + Rice 1/2Waris H + Si

7 淡水産紅藻用培地

Bold 3N*

8 シャジクモ類用培地

mSWC–2 ( Modified SWC–2 ) SWC–1 SWCN–1 SWCN–2 SWCN–3 SWCN–4 1/3 Herbst ASW*

1 淡水産、陸生、温泉産、塩水産藻類用培地

AAF–6*
Acid–CSi/5*
AF–6*
AF–6/2*
AFAC*
Allen*
BBM
BG–11*
BG–11 (寒天) *
C*
C (寒天) *
C+10% Seawater
C/6G
CA*
CAM*
Carefoot*
CB*
CB–V*
CC
CSi*
CSi + Cu (軟寒天)*
CSi/5*
CT*
CYT*
DH + Fe (寒天)
DY–V*
HUT*
HUT (軟寒天)*
M–11*
MA*
MAF–6*
M–Allen*
M–Allen( + U )*
MBM*
M Chu No. 10*
MDM*
MDM (寒天)*
MG*
MGM
Modified acetate medium ( mAC )*
Modified M–1 ( mM–1 )
MW*
MW/5*
N–Free (寒天)*
O (軟寒天)*
P 35*
Pro (寒天)*
SOT*
SW
TAP (寒天)*
Tre (寒天)*
URO*
URO–H*
URO–T*
VT*
VTAC*
VTYT
W

2 海産および汽水産藻類用培地

BESM*
BESM 2*
ESM*
ESM (寒天)*
ESM2*
f/2 + NH4Cl*
f/2*
IMK
K*
K/2*
M–ASP7
MF
mIMR* ( modified IMR )
MKM
MNK*
PRO–99*
WESM*

3 淡水産藻類用無菌検査培地

B–I
B–II
B–III
B–IV
B–V
YT

4 海産藻類用無菌検査培地

Bf/2
MM 23
STP*

5 微量金属、ビタミン混液、土壌浸出液

A2 Fe stock solution*
A2 trace element stock solution*
A5 solution*
Allen metals*
C–Source Mix II*
D stock medium
D trace mix
DY trace metal solution*
Fe ( as EDTA; 1:1 molar )*
Fe solution
f/2 metals*
Hutner ’s trace elements
K metals*
K/2 metals*
mIMR trace metals*
mM–1 Trace elements
mTYGM–9*
P II metals*
P IV metals*
PN metals
PRO–99 trace metals*
Soil extract
Trace metal mix A5 + Co*
Vitamin mix S3

6 原生動物用培地

AF–6 + Wheat
ESM + mTYGM–9 + Rice
f/2 + mTYGM–9 + Rice
f/2 + Wheat
Helio*
LE
PYG*
SUY*
SUY 1/10*
SUY 1/10 + mTYGM–9 + Rice
SUY 1/10 + Wheat
URO + Wheat
URO–H + Wheat
UYTS + Rice

7 淡水産紅藻用培地

Bold 3N*

8 シャジクモ類用培地

mSWC–2 ( Modified SWC–2 )
SWC–1
SWCN–1
SWCN–2
SWCN–3
SWCN–4
1/3 Herbst ASW*
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1 淡水産、陸生、温泉産、塩水産藻類用培地
AAF–6*

印刷

Prepare as for AF–6 medium but adjust to pH 5.5–5.8 .

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1 淡水産、陸生、温泉産、塩水産藻類用培地
Acid–CSi/5*

印刷

Dilute CSi 1 ) medium with distilled water to one–fifth. Adjust to pH 3 with sulfuric acid.
1 ) See CSi

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1 淡水産、陸生、温泉産、塩水産藻類用培地
AF–6*

印刷

NaNO314 mg
NH4NO32.2 mg
MgSO4 · 7H2O3 mg
KH2PO41 mg
K2HPO40.5 mg
CaCl2 · 2H2O1 mg
CaCO3 1 ) 1 mg
Fe–citrate0.2 mg
Citric acid0.2 mg
Biotin0.2 μg
Thiamine HCl1 μg
Vitamin B60.1 μg
Vitamin B120.1 μg
Trace metals 1 )0.5 mL
Distilled water99.5 mL
pH 6.6 2 ) 
1 ) In the NIES–Collection, CaCO3 is removed and PIV metals are used instead of Trace metals.
2 ) In the NIES–Collection, 40 mg MES is added and pH is adjusted to 6.6.
Reference
Kato, S. 1982 Laboratory culture and morphology of Colacium vesiculosum Ehrb. ( Euglenophyceae ). Jpn. J. Phycol., 30, 63-67 ( in Japanese with English summary ).

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1 淡水産、陸生、温泉産、塩水産藻類用培地
AF–6/2*

印刷

AF–6 1 ) medium is diluted with distilled water to one-half.
1 ) See AF–6

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1 淡水産、陸生、温泉産、塩水産藻類用培地
AFAC*

印刷

To 100 mL AF–6 1 ) medium add 20 mg sodium acetate.
1 ) See AF–6

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1 淡水産、陸生、温泉産、塩水産藻類用培地
Allen*

印刷

( NH4 )2SO4 132 mg
KH2PO427.2 mg
MgSO4 · 7H2O24.6 mg
CaCl2 · 2H2O7.4 mg
Allen metals 1 )0.01 mL
Distilled water99.9 mL
pH 2.5 2 ) 
1 ) See Allen metals
2 ) pH is adjusted to 2.5 with 0.5 mol / L H2SO4.
Reference
Allen, M. B. 1959 Studies with Cyanidium caldarium, an anomalously pigmented chlorophyte. Arch. Mikrobiol., 32, 270-277.
Starr, R. C., Zeikus, J. A. 1987 UTEX - The culture collection of algae at the University of Texas at Austin. J. Phycol., 23, Suppl. to Sept., 1-106.

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1 淡水産、陸生、温泉産、塩水産藻類用培地
BBM

印刷

NaNO3 25 mg
KH2PO417.5 mg
K2HPO410 mg
MgSO4 · 7H2O7.5 mg
CaCl2 · 2H2O2.5 mg
NaCl2.5 mg
KOH3.1 mg
FeSO4 · 7H2O0.498 mg
H3BO31.142 mg
ZnSO4 · 7H2O0.882 mg
MnCl2 · 7H2O0.144 mg
MoO30.071 mg
CuSO4 · 5H2O0.157 mg
Co( NO3 )2 · 6H2O0.049 mg
Na2EDTA5 mg
Distilled water 100 mL
Reference
Bischoff, H. W., Bold, H. C. 1963 Some soil algae from enchanted rock and related algal species. Phycological Studies IV.,Univ. No. 6318, Texas, p. 95.

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1 淡水産、陸生、温泉産、塩水産藻類用培地
BG–11*

印刷

NaNO3150 mg
K2HPO4 · 3H2O4 mg
MgSO4 · 7H2O7.5 mg
CaCl2 · 2H2O3.6 mg
Citric acid0.6 mg
Ferric ammonium citrate0.6 mg
Na2EDTA – Mg0.1 mg
Na2CO32 mg
Trace metal mix A5 + Co 1 ) 0.1 mL
Agar1.5 g
Distilled water99.9 mL
pH 7.4 
1 ) See Trace metal mix A5 + Co

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1 淡水産、陸生、温泉産、塩水産藻類用培地
C*

印刷

Ca( NO3 )2 · 4H2O15 mg
KNO310 mg
β–Na2glycerophosphate · 5H2O5 mg
MgSO4 · 7H2O 4 mg
Vitamin B120.01 μg
Biotin0.01 μg
Thiamine HCl1 μg
PIV metals 1 )0.3 mL
Tris ( hydroxymethyl ) aminomethane50 mg
Distilled water99.7 mL
pH 7.5 
Add 1.5 g agar to 100 mL of medium to give a solid medium.
1 ) See PIV metals
Reference
Ichimura, T. 1971 Sexual cell division and conjugation-papilla formation in sexual reproduction of Closterium strigosum. In Proceedings of the Seventh International Seaweed Symposium, University of Tokyo Press, Tokyo, p. 208-214.

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1 淡水産、陸生、温泉産、塩水産藻類用培地
C+10% Seawater ( N. Tezuka, unpubl. )

印刷

C 1 ) medium with 10% filtered seawater.
1 ) See C

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1 淡水産、陸生、温泉産、塩水産藻類用培地
C/6G

印刷

Mix 1 volume of C 1 ) medium and 5 volumes of Lake Nojiri water ( sterilized through GF/F filter, and store at 5°C ).
1 ) See C

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1 淡水産、陸生、温泉産、塩水産藻類用培地
CA*

印刷

Ca( NO3 )2 · 4H2O2 mg
KNO310 mg
NH4NO35 mg
β–Na2glycerophosphate · 5H2O3 mg
MgSO4 · 7H2O2 mg
Vitamin B120.01 μg
Biotin0.01 μg
Thiamine HCl1 μg
PIV metals 1 )0.1 mL
Fe ( as EDTA; 1:1 molar ) 2 )0.1 mg
HEPES40 mg
Distilled water99.9 mL
pH 7.2 
1 ) See PIV metals
2 ) See Fe ( as EDTA; 1:1 molar )
Reference
Ichimura, T., Watanabe, M. 1974 The Closterium calosporum complex from the Ryukyu Islands - Variation and taxonomical problems. Mem. Natn. Sci. Mus. Tokyo, 7, 89-102, pls. 13-14.

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1 淡水産、陸生、温泉産、塩水産藻類用培地
CAM*

印刷

CA 1 ) medium with pH adjusted to 6.5 by buffering with MES instead of HEPES.
1 ) See CA

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1 淡水産、陸生、温泉産、塩水産藻類用培地
Carefoot*

印刷

NaNO324.7 mg
CaCl2 · 2H2O1.1 mg
MgSO4 · 7H2O4.7 mg
K2HPO40.9 mg
KH2PO42.3 mg
NaCl1.5 mg
PIV metals 1 )0.5 mL
Distilled water99.5 mL
pH 7.5 
In the NIES–Collection, 0.02 μg vitamin B12, 0.02 μg biotin and 2 μg thiamine HCl are added to this medium.
1 ) See PIV metals
Reference
Carefoot, J. R. 1968 Culture and heterotrophy of the freshwater dinoflagellate, Peridinium cinctum fa. ovoplanum Lindeman. J. Phycol., 4, 129-131.

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1 淡水産、陸生、温泉産、塩水産藻類用培地
CB*

印刷

To C 1 ) medium add Bicine instead of Tris ( hydroxymethyl ) aminomethane, and adjust pH to 9.0.
1 ) See C

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1 淡水産、陸生、温泉産、塩水産藻類用培地
CB–V*

印刷

Make B–V 1 ) medium with C 2 ) medium.
1 ) See B–V
2 ) See C

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1 淡水産、陸生、温泉産、塩水産藻類用培地
CC

印刷

C 1 ) medium with pH adjusted to 3.0 by buffering with 1,2,3,4–cyclopentane tetracarboxylic acid instead of Tris ( hydroxymethyl ) aminomethane.
1 ) See C
Reference
Ichimura, T., Itoh, T. 1977 17. Preservation methods of microalgae ( I ) [17. Bisaisôrui no hozonhô ( I )]. In Preservation methods of microorganisms [ Biseibutsu Hozonhô], Ed. by Nei, T., University of Tokyo Press, Tokyo, p. 355-373 ( in Japanese without English title ).

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1 淡水産、陸生、温泉産、塩水産藻類用培地
CSi*

印刷

C 1 ) medium with pH adjusted to 7.0 by buffering with 50mg HEPES instead of Tris ( hydroxymethyl ) aminomethane. Thereafter, 10 mg Na2SiO3 · 9H2O is added.
1 ) See C

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1 淡水産、陸生、温泉産、塩水産藻類用培地
CSi + Cu*

印刷

To 100 mL CSi 1 ) medium add 0.25 mg CuSO4 · 5H2O and 100 mg agar.
1 ) See CSi

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1 淡水産、陸生、温泉産、塩水産藻類用培地
CSi/5*

印刷

Dilute CSi 1 ) medium with distilled water to 1/5.
1 ) See CSi

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1 淡水産、陸生、温泉産、塩水産藻類用培地
CT*

印刷

C 1 ) medium with pH adjusted to 8.2 by buffering with 40mg TAPS instead of Tris ( hydroxymethyl ) aminomethane.
1 ) See C
Reference
Watanabe, M. M., Ichimura, T. 1977 Fresh- and salt-water forms of Spirulina platensis in axenic cultures. Bull. Jpn. Soc. Phycol., 25, Suppl. ( Mem. Iss. Yamada ), 371-377.

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1 淡水産、陸生、温泉産、塩水産藻類用培地
CYT*

印刷

To 100 mL C 1 ) medium add 100 mg yeast extract and 200 mg tryptone.
1 ) See C

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1 淡水産、陸生、温泉産、塩水産藻類用培地
DH + Fe ( I.I. Brown, unpubl. )

印刷

D stock medium 1 )5 mL
HEPES0.12 mg
FeCl3 · 6H2O1.14 mg
Distilled water95 mL
pH 8.24 – 8.26 
After autoclaving, keep in room temperature overnight. Next day, adjust pH to 7.5–7.6 and add 1.5 g agar.
1 ) See D stock medium

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1 淡水産、陸生、温泉産、塩水産藻類用培地
DY–V*

印刷

MES20 mg
MgSO4 · 7H2O5 mg
KCl0.3 mg
NH4Cl0.27 mg
NaNO32 mg
β–Na2glycerophosphate · 5H2O0.22 mg
H3BO30.08 mg
Na2EDTA · 2H2O0.8 mg
Na2SiO3 · 9H2O1.4 mg
FeCl3 · 6H2O0.1 mg
CaCl2 · 2H2O7.5 mg
Vitamin B120.05 μg
Biotin0.05 μg
Thiamine HCl10 μg
DY trace metal solution 1 )0.1 mL
Distilled water99.9 mL
pH 6.8 
1 ) See DY trace metal solution

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1 淡水産、陸生、温泉産、塩水産藻類用培地
HUT*

印刷

KH2PO42 mg
MgSO4 · 7H2O2.5 mg
Sodium acetate40 mg
Potassium citrate4 mg
Polypeptone60 mg
Yeast extract40 mg
Vitamin B120.05 μg
Thiamine HCl0.04 mg
Distilled water100 mL
pH 6.4 
Add 150 mg agar to 100 mL of medium to give a semi-solid medium.
Reference
Ichimura, T. 1979 2. Isolation and culture methods of algae. 2.5.B. Freshwater algae [2. Sôrui no bunri to baiyôhô. 2.5.B. Tansui sôrui]. In Methods in Phycological Studies [Sôrui Kenkyûhô], Eds. by Nishizawa, K. & Chihara, M., Kyoritsu Shuppan, Tokyo, p. 301 (in Japanese without English title).

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1 淡水産、陸生、温泉産、塩水産藻類用培地
M–11

印刷

NaNO310 mg
K2HPO41 mg
MgSO4 · 7H2O7.5 mg
CaCl2 · 2H2O4 mg
Na2CO33 mg
FeSO4 · 7H2O0.1 mg
Na2EDTA · 2H2O0.1 mg
Distilled water100 mL
pH 8.0 
Reference
Hagiwara, T., Yagi, O., Takamura, Y., Sudo, R. 1984 Isolation of bacteria-free Microcystis aeruginosa from Lake Kasumigaura. Jpn. J. Water Poll. Res., 7, 437-442 ( in Japanese with English summary ).
Yagi, O., Okada, M., Sudo, R. 1979 Cultivation of Microcystis and red-tide-organisms. Res. Rep. Natl. Inst. Environ. Stud., No. 6, 223-229 ( in Japanese with English summary ).

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1 淡水産、陸生、温泉産、塩水産藻類用培地
MA*

印刷

Ca( NO3 )2 · 4H2O5 mg
KNO310 mg
NaNO35 mg
Na2SO44 mg
MgCl2 · 6H2O5 mg
β–Na2glycerophosphate · 5H2O10 mg
Na2EDTA · 2H2O0.5 mg
FeCl3 · 6H2O0.05 mg
MnCl2 · 4H2O0.5 mg
ZnCl20.05 mg
CoCl2 · 6H2O0.5 mg
Na2MoO4 · 2H2O0.08 mg
H3BO32 mg
Bicine50 mg
Distilled water100 mL
pH 8.6 
Reference
Ichimura, T. 1979 2. Isolation and culture methods of algae. 2.5.B. Freshwater algae [2. Sôrui no bunri to baiyôhô. 2.5.B. Tansui sôrui]. In Methods in Phycological Studies [Sôrui Kenkyûhô], Eds. by Nishizawa, K. & Chihara, M., Kyoritsu Shuppan, Tokyo, p. 294-305 ( in Japanese without English title ).

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1 淡水産、陸生、温泉産、塩水産藻類用培地
MAF–6*

印刷

To 100 mL of AF–6 1 ) medium add 10 mg glucose and 10 mg sodium acetate.
1 ) See AF–6

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1 淡水産、陸生、温泉産、塩水産藻類用培地
M–Allen*

印刷

( NH4 )2SO4 262 mg
KH2PO454 mg
MgSO4 · 7H2O50 mg
CaCl2 · 2H2O14 mg
A2 trace element stock solution 1 )0.2 mL
Distilled water99.4 mL
pH 2.5 2 ) 
After autoclaving, add 0.4 mL of A2 Fe stock solution 3 ) ( filter-sterilized ).
1 ) See A2 trace element stock solution
2 ) pH is adjusted to 2.5 with 0.5 mol / L H2SO4.
3 ) See A2 Fe stock solution
Indicated as “MA” medium in reference.

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1 淡水産、陸生、温泉産、塩水産藻類用培地
M–Allen( + U )*

印刷

To 100 mL M–Allen 1 ) medium add 50 mg uracil.
1 ) See M–Allen

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1 淡水産、陸生、温泉産、塩水産藻類用培地
MBM*

印刷

KNO325 mg
MgSO4 · 7H2O7.5 mg
K2HPO47.5 mg
KH2PO417.5 mg
NaCl2.5 mg
CaCl2 · 2H2O1 mg
Fe solution 1 )0.1 mL
A5 solution 2 )0.1 mL
Agar1.5 g
Distilled water99.8 mL
pH 6.0 
1 ) See Fe solution.
2 ) See A5 solution.
Indicated as “Modified Bristol medium” in reference.
Reference
Ichimura, T., Itoh, T. 1977 17. Preservation methods of microalgae ( I ) [17. Bisaisôrui no hozonhô ( I )]. In Preservation methods of microorganisms [Biseibutsu Hozonhô], Ed. by Nei, T., University of Tokyo Press, Tokyo, p. 355-373 ( in Japanese without English title ).

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1 淡水産、陸生、温泉産、塩水産藻類用培地
M Chu No. 10

印刷

Ca( NO3 )2 · 4H2O2.0 mg
KH2PO40.62 mg
MgSO4 · 7H2O2.5 mg
Na2CO32 mg
Na2SiO3 · 9H2O2.5 mg
HCl ( 1 mol / L ) 1 )0.025 mL
Na2EDTA · 2H2O0.2 mg
FeCl3 · 6H2O0.1 mg
H3BO30.248 mg
MnCl2 · 4H2O0.139 mg
( NH4 )6Mo7O24 · 4H2O0.1 mg
Vitamin B121 μg
Thiamine HCl0.1 μg
Biotin0.1 μg
Distilled water100 mL
1 ) In the NIES–Collection, pH is adjusted to 7.6 with 1 mol / L HCl.
Reference
Chu, S. P. 1942 The influence of the mineral composition of the medium on the growth of planktonic algae. Part I. Methods and culture media. J. Ecol., 30, 284-325.

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1 淡水産、陸生、温泉産、塩水産藻類用培地
MDM*

印刷

KNO3100 mg
MgSO4 · 7H2O25 mg
K2HPO425 mg
NaCl 10 mg
CaCl2 · 2H2O1 mg
Fe solution 1 )0.1 mL
A5 solution 2 )0.1 mL
Agar1.5 g
Distilled water99.8 mL
pH 8.0 
1 ) See Fe solution
2 ) See A5 solution

Reference
Watanabe, A. 1960 List of algal strains in collection at the Institute of Applied Micro-biology, University of Tokyo. J. Gen. Appl. Microbiol., 6, 283-292.

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1 淡水産、陸生、温泉産、塩水産藻類用培地
MG*

印刷

Ca ( NO3 )2·4H2O2 mg
KNO310 mg
β–Na2 glycerophosphate·5H2O3 mg
MgSO4 · 7H2O2 mg
Vitamin B120.01 µg
Biotin0.01 µg
Thiamine HCl1 µg
PIV metals 1 )0.1  mL
Fe ( as EDTA; 1:1 molar ) 2 )0.1  mL
HEPES40 mg
Distilled water99.8  mL
pH 7.2 
1 ) See PIV metals
2 ) See Fe ( as EDTA; 1:1 molar )  
Reference
Ichimura, T. 1973 The life cycle and its control in some species of Closterium, with special reference to the biological species problems. Thesis D. Sci., University of Tokyo, 69 pp., 11 tables, 40 figs.

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1 淡水産、陸生、温泉産、塩水産藻類用培地
MGM

印刷

MG 1 ) medium with pH adjusted to 6.5 by buffering with MES instead of HEPES.
1 ) See MG

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1 淡水産、陸生、温泉産、塩水産藻類用培地
Modified acetate medium ( mAC )

印刷

To 100 mL AF–6 1 ) medium, add 40mg glucose, yeast extract, tryptone, and sodium acetate.
1 ) See AF–6
Reference
Nozaki, H., Wanababe, M. M. & Aizawa, K. 1995 Morphology and paedogamous sexual reproduction in Chlorogonium capillatum sp. nov. ( Volvocales, Chlorophyta ). J. Phycol., 31, 655-663.

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1 淡水産、陸生、温泉産、塩水産藻類用培地
Modified M–1 ( mM–1 )

印刷

CaCl2 · 2H2O0.5 mg
NaNO32.5 mg
NH4Cl0.5 mg
CaSO4 · 2H2O0.4 mg
MgSO4 · 7H2O0.5 mg
Na2SiO3 · 9H2O0.2 mg
Fe ( as EDTA; 1:1 molar ) 1 )25 mL
mM–1 Trace elements 2 )0.1 mL
K2HPO46.96 mg
KH2PO4266.5 mg
Distilled water74.9 mL
pH 5.1 – 5.3 
1 ) See Fe ( as EDTA; 1:1 molar )
2 ) See mM–1 Trace elements
Reference
Hoham, R. W., Berman, J. D., Rogers, H. S., Felio, J. H., Ryba, J. B., Miller, P. R. 2006 Two new species of green snow algae from Upstate New York, Chloromonas chenangoensis sp. nov. and Chloromonas tughillensis sp. nov. ( Volvocales, Chlorophyceae ) and the effects of light on their life cycle development. Phycologia, 45, 319-330.

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1 淡水産、陸生、温泉産、塩水産藻類用培地
MW

印刷

Urea0.85 mg
NaNO30.17 mg
NH4Cl0.042 mg
Ca( NO3 )2 · 4H2O10 mg
CaCO31 mg
CaCl2 · 2H2O1.4 mg
KNO31 mg
KHCO30.9 mg
β–Na2glycerophosphate · 5H2O2 mg
MgSO4 · 7H2O1.5 mg
PIV metals 1 )0.05 mL
Vitamin B120.02 μg
Thiamine HCl2 μg
Biotin0.02 μg
Glycylglycine10 mg
Distilled water100 mL
pH 7.2 
1 ) See PIV metals
Reference
Sako, Y., Ishida, Y., Kadota, H., Hata, Y. 1984 Sexual reproduction and cyst formation in the freshwater dinoflagellate Peridinium cunningtonii. Bull. Jpn. Soc. Sci. Fish., 50, 743-750.

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1 淡水産、陸生、温泉産、塩水産藻類用培地
MW/5

印刷

MW 1 ) medium is diluted with distilled water to 1/5.
1 ) See MW

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1 淡水産、陸生、温泉産、塩水産藻類用培地
N–Free*

印刷

NaCl7 mg
MgSO4 · 7H2O38 mg
CaCl2 · 2H2O10.6 mg
K2HPO460 mg
Fe2( SO4 )3 · 6H2O1 mg
Na2EDTA · 2H2O2.7 mg
H3BO30.3 mg
MnSO4 · 4H2O 1 )0.2 mg
Na2MoO4 · 2H2O0.8 mg
ZnSO4 · 7H2O0.03 mg
CuSO4 · 5H2O8 μg
CoCl2 · 6H2O3.7 μg
Agar1.5 g
Distilled water100 mL
pH 7.5 
1 ) In the NIES–Colletion, 0.2 mg MnSO4 · 4H2O is replaced by 0.22 mg MnSO4 · 5H2O.

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1 淡水産、陸生、温泉産、塩水産藻類用培地
O*

印刷

Glucose100 mg
Tryptone100 mg
Yeast extract100 mg
Beef extract 1 )50 mg
Agar150 mg
Distilled water100 ml
1 ) In the NIES-Collection, beef extract is removed.
Indicated as “Ochomonas medium” in reference.
Reference
Ichimura, T., Itoh, T. 1977 17. Preservation methods of microalgae ( I ) [17. Bisaisôrui no hozonhô ( I )]. In Preservation methods of microorganisms [Biseibutsu Hozonhô], Ed. by Nei, T., University of Tokyo Press, Tokyo, p. 355-373 ( in Japanese without English title ).
Starr, R. C. 1964 The culture collection of algae at Indiana University. Amer. J. Bot., 51, 1013-1044.

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1 淡水産、陸生、温泉産、塩水産藻類用培地
P 35*

印刷

NH4NO310 mg
MgSO4 · 7H2O4 mg
KCl5 mg
CaCl2 · 2H2O7.4 mg
β–Na2glycerophosphate · 5H2O5 mg
Sodium acetate100 mg
Vitamin B120.01 μg
Biotin0.01 μg
Thiamine HCl1 μg
PIV metals 1 )0.3 mL
Tris ( hydroxymethyl ) aminomethane50 mg
Distilled water99.7 mL
pH 8.0 
1 ) See PIV metals
Reference
Ichimura, T. 1979 2. Isolation and culture methods of algae. 2.5.B. Freshwater algae [2. Sôrui no bunri to baiyôhô. 2.5.B. Tansui sôrui]. In Methods in Phycological Studies [Sôrui Kenkyûhô ], Eds. by Nishizawa, K. & Chihara, M., Kyoritsu Shuppan, Tokyo, p. 294-305 ( in Japanese without English title ).

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1 淡水産、陸生、温泉産、塩水産藻類用培地
Pro*

印刷

To 100 mL MBM 1 ) medium add 100 mg proteose peptone.
1 ) See MBM
Indicated as “Proteose medium” in reference.
Reference
Ichimura, T., Itoh, T. 1977 17. Preservation methods of microalgae ( I ) [17. Bisaisôrui no hozonhô ( I )]. In Preservation methods of microorganisms [Biseibutsu Hozonhô], Ed. by Nei, T., University of Tokyo Press, Tokyo, p. 355-373 ( in Japanese without English title ).
Starr, R. C. 1964 The culture collection of algae at Indiana University. Amer. J. Bot., 51, 1013-1044.

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1 淡水産、陸生、温泉産、塩水産藻類用培地
SOT*

印刷

NaHCO31.68 g
K2HPO450 mg
NaNO3250 mg
K2SO4100 mg
NaCl100 mg
MgSO4 · 7H2O20 mg
CaCl2 · 2H2O4 mg
FeSO4 · 7H2O1 mg
Na2EDTA · 2H2O8 mg
A5 solution 1 )0.1 mL
Distilled water99.9 mL
1 ) See A5 solution
Reference
Ogawa, T., Terui, G. 1970 Studies on the growth of Spirulina platensis. ( I ) On the pure culture of Spirulina platensis. J. Ferment. Technol., 48, 361-367.

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1 淡水産、陸生、温泉産、塩水産藻類用培地
SW

印刷

Put a small amount of dried soil into a test tube and add 20mL distilled water.
Reference
Pringsheim, E. G. 1946 The biphasic or soil-water culture method for growing algae and flagellata. J. Ecol., 33, 193-204.

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1 淡水産、陸生、温泉産、塩水産藻類用培地
TAP*

印刷

NH4Cl40 mg
CaCl2 · 2H2O5.1 mg
MgSO4 · 7H2O10 mg
K2HPO411.9 mg
KH2PO46.03 mg
Hutner ’s trace elements 1 )0.1 mL
Acetic acid0.1 mL
Tris ( hydroxymethyl ) aminomethane242 mg
Agar1.5 g
Distilled water99.8 mL
1 ) See Hutner ’s trace elements

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1 淡水産、陸生、温泉産、塩水産藻類用培地
Tre*

印刷

To 100 mL MBM 1 ) medium add 1 g proteose peptone and 2 g glucose.
1 ) See MBM
Indicated as “Trebouxia medium” in reference.
Reference
Ichimura, T., Itoh, T. 1977 17. Preservation methods of microalgae ( I ) [17. Bisaisôrui no hozonhô ( I )]. In Preservation methods of microorganisms [Biseibutsu Hozonhô], Ed. by Nei, T., University of Tokyo Press, Tokyo, p. 355-373 ( in Japanese without English title ).
Starr, R. C. 1964 The culture collection of algae at Indiana University. Amer. J. Bot., 51, 1013-1044.

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1 淡水産、陸生、温泉産、塩水産藻類用培地
URO*

印刷

NH4NO30.5 mg
β–Na2glycerophosphate · 5H2O0.4 mg
MgSO4 · 7H2O1 mg
CaCl2 · 2H2O1 mg
KCl0.1 mg
Thiamine HCl1 μg
Vitamin B120.01 μg
Biotin0.01 μg
Fe-EDTA0.05 mg
PIV metals 1 )0.1 mL
Distilled water99.9 mL
pH 7.5 2 ) 
1 ) See PIV metals
2 ) pH is adjusted to 7.5 with 0.1 mol / L HCl.
Reference
Kimura, B., Ishida, Y. 1985 Photophagotrophy in Uroglena americana, Chrysophyceae. Jpn. J. Limnol., 46, 315-318.
Nakahara, H., Sako, Y. 1987 2. Life history of freshwater phytoplankton [2. Tansui syokubutsu purankuton no seikatsushi]. In Freshwater red tide [Tansui Akashio ], Ed. by Kadota, H., Kôseisya-Kôseikaku, Tokyo, p. 21-77 ( in Japanese without English title ).

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1 淡水産、陸生、温泉産、塩水産藻類用培地
URO–H*

印刷

To 100 mL URO 1 ) medium add 40 mg HEPES.
1 ) See URO

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1 淡水産、陸生、温泉産、塩水産藻類用培地
URO–T*

印刷

To 100 mL URO 1 ) medium add 50 mg Tris ( hydroxymethyl ) aminomethane.
1 ) See URO

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1 淡水産、陸生、温泉産、塩水産藻類用培地
VT*

印刷

Ca( NO3 )2 · 4H2O11.78 mg
β-Na2glycerophosphate · 5H2O5 mg
MgSO4 · 7H2O4 mg
KCl5 mg
Vitamin B120.01 μg
Biotin0.01 μg
Thiamine HCl1 μg
PIV metals 1 )0.3 mL
Glycylglycine50 mg
Distilled water99.7 mL
pH 7.5 
1 ) See PIV metals
Reference
Provasoli, L., Pintner, I. J. 1959 Artificial media for fresh-water algae: problems and suggestions. In The Ecology of Algae. Spec. Pub. No. 2,, Eds. by Tryon, C. A., Jr. & Hartmann, R. T., Pymatuning Laboratory of Field Biology, University of Pittsburgh, Pittsburgh, p. 84-96.
Starr, R. C. 1973 Special methods-dry soil samples. In Handbook of Phycological Methods. Culture Methods and Growth Measurements, Ed. by Stein, J. R., Cambridge University Press, Cambridge, p. 159-167.

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1 淡水産、陸生、温泉産、塩水産藻類用培地
VTAC*

印刷

To 100 mL VT 1 ) medium add 20 mg sodium acetate.
1 ) See VT
Reference
Nozaki, H., Kuroiwa, H., Mita, T., Kuroiwa, T. 1989 Pleodorina japonica sp. nov. ( Volvocales, Chlorophyta ) with bacteria-like endosymbionts. Phycologia, 28, 252-267.

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1 淡水産、陸生、温泉産、塩水産藻類用培地
VTYT

印刷

To 100 mL VT 1 ) medium add 10 mg yeast extract and 20 mg tryptone.
1 ) See VT
Reference
Ichimura, T., Itoh, T. 1977 17. Preservation methods of microalgae ( I ) [17. Bisaisôrui no hozonhô ( I ) ]. In Preservation methods of microorganisms [Biseibutsu Hozonhô], Ed. by Nei, T., University of Tokyo Press, Tokyo, p. 355-373 ( in Japanese without English title ).

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1 淡水産、陸生、温泉産、塩水産藻類用培地
W

印刷

Ca( NO3 )2 · 4H2O10 mg
KNO31 mg
MgSO4 · 7H2O1.5 mg
β–Na2glycerophosphate · 5H2O2 mg
Urea1.7 mg
Thiamine HCl0.2 μg
Vitamin B120.002 μg
Biotin0.002 μg
PIV metals 1 )0.05 mL
Glycylglycine10 mg
Distilled water99.95 mL
pH 7.5 
1 ) See PIV metals
Reference
Watanabe, M. M. 1983 Growth characteristics of freshwater red tide alga, Peridinium based on axenic culture. Establishment of synthetic culture medium [Junsuibaiyôhô niyoru tansuiakashio Peridinium no zôsyokutokusei no kaiseki-gôseibaichi no kakuritsu ]. Res. Data Natl. Inst. Environ. Stud., No. 24, 111-121 ( in Japanese without English title ).

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2 海産および汽水産藻類用培地
BESM*

印刷

Make diluted seawater by mixing 27.5 mL seawater and 70 mL distilled water. Make ESM 1 ) medium by using this diluted seawater instead of original seawater.
1 ) See ESM

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2 海産および汽水産藻類用培地
BESM 2*

印刷

Make diluted seawater by mixing 47.5 mL seawater and 50 mL distilled water. Make ESM 1 ) medium by using this diluted seawater instead of original seawater.
1 ) See ESM

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2 海産および汽水産藻類用培地
ESM*

印刷

NaNO312 mg
K2HPO40.5 mg
Vitamin B120.1 μg
Biotin0.1 μg
Thiamine HCl10 μg
Fe – EDTA25.9 μg
Mn – EDTA33.2 μg
Tris ( hydroxymethyl ) aminomethane 100 mg
Soil extract 1 )2.5 mL
Seawater97.5 mL
pH 8.0 
Add 1.5 g agar to 100 mL of medium to give a solid medium.
1 ) The amount of Soil extract depends on the quality of the soil. In the NIES–Collection, Soil extract was reduced from 5 mL to 2.5 mL after 2002. See Soil extract.
Reference
Okaichi, T., Nishio, S., Imatomi, Y. 1982 Collection and mass culture [Shiryô no saisyu to baiyô]. In Toxic phytoplankton - Occurrence, mode of action, and toxins [Yûdoku Purankuton -Hassei, Sayôkikô, Dokuseibun ], Ed. by Jpn. Fish. Soc., Kôseisya-Kôseikaku, Tokyo, p. 22-34 ( in Japanese without English title ).

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2 海産および汽水産藻類用培地
ESM2*

印刷

Prepare as for 100 mL ESM 1 ) medium with 95.5mL instead of 97.5 mL seawater and with 5 mL instead of 2.5 mL Soil extract 2 ).
1 ) See ESM
2 ) See Soil extract

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2 海産および汽水産藻類用培地
f/2*

印刷

NaNO37.5 mg
NaH2PO4 · 2H2O0.6 mg
Vitamin B120.05 μg
Biotin0.05 μg
Thiamine HCl10 μg
Na2SiO3 · 9H2O1 mg
f/2 metals 1 )0.1 mL
Seawater99.9 mL
1 ) See f/2 metals
Reference
Guillard, R. R. L., Ryther, J. H. 1962 Studies of marine planktonic diatoms. I. Cyclotella nana Hustedt, and Detonula confervacea ( Cleve ) Gran. Can. J. Microbiol., 8, 229-239.

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2 海産および汽水産藻類用培地
f/2 + NH4Cl*

印刷

To 100 mL f/2 1 ) medium add 2.67 mg NH4Cl.
1 ) See f/2

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2 海産および汽水産藻類用培地
IMK

印刷

Into 100 mL seawater dissolve 25.2 mg powder medium of Daigo IMK ( Nihon Pharmaceutical Co., Ltd. ). In the NIES–Collection, IMK medium is used after autoclaving ( 121°C, 20 min ).

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2 海産および汽水産藻類用培地
K*

印刷

Tris-base (  pH 7.2) 1 ) 0.1 mL
NaNO37.5 mg
NH4Cl 0.267 mg
β–Na2glycerophosphate · 5H2O0.216 mg
H2SeO30.129 mg
Vitamin B120.05 μg
Biotin0.005 μg
Thiamine HCl0.01 mg
Na2SiO3 · 9H2O2 )1.535 mg
K metals 3 )0.1 mL
Seawater99.8 mL
1 ) To 100 mL of Distilled water add 12.11 g Tris-base, and pH is adjusted to 7.2.
2 ) In the NIES-Collection, Na2SiO3 · 9H2O is removed.
3 ) See K metals
Reference
Keller, M. D., Guillard, R.R.L. 1985 Factors significant to marine diatom culture. pp. 113-6. In Anderson, D.M., White, A.W., Baden, D.G. (eds.) Toxic Dinoflagellates. Elsevier, New York.
Keller, M.D., Selvin, R.C., Claus, W., Guillard, R.R.L. 1987. Media for the culture of oceanic ultraphytoplankton. J. Phycol., 23: 633-638.

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2 海産および汽水産藻類用培地
K/2 (K/2-modified by Ian Probert)*

印刷

NaNO32.44 mg
NH4Cl 0.0267 mg
KH2PO40.244 mg
Fe-EDTA0.215 mg
Vitamin B120.05 μg
Biotin0.0005 μg
Thiamine HCl0.01 mg
K/2 metals 1 )0.05 mL
Seawater99.95 mL
pH 8.2 
K medium is diluted half strength and modified by Ian. This medium should not be autoclaved but filter-sterilized.
1 ) See K/2 metals
Reference
Keller, M. D., Guillard, R.R.L. 1985 Factors significant to marine diatom culture. pp. 113-6. In Anderson, D.M., White, A.W., Baden, D.G. (eds.) Toxic Dinoflagellates. Elsevier, New York.
Keller, M.D., Selvin, R.C., Claus, W., Guillard, R.R.L. 1987. Media for the culture of oceanic ultraphytoplankton. J. Phycol., 23: 633-638.

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2 海産および汽水産藻類用培地
K/2ET*

印刷

NaNO32.44 mg
NH4Cl 0.0267 mg
KH2PO40.244 mg
Fe-EDTA0.215 mg
Vitamin B120.05 μg
Biotin0.0005 μg
Thiamine HCl0.01 mg
K/2 metals 1 )0.05 mL
Soil extract 2 )1.5 mL
Seawater98.45 mL
pH 8.2 
K medium is diluted half strength and modified by Ian. This medium should not be autoclaved but filter-sterilized.
1 ) See K/2 metals
2 ) See Soil extract
Reference
Keller, M. D., Guillard, R.R.L. 1985 Factors significant to marine diatom culture. pp. 113-6. In Anderson, D.M., White, A.W., Baden, D.G. (eds.) Toxic Dinoflagellates. Elsevier, New York.
Keller, M.D., Selvin, R.C., Claus, W., Guillard, R.R.L. 1987. Media for the culture of oceanic ultraphytoplankton. J. Phycol., 23: 633-638.

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2 海産および汽水産藻類用培地
M–ASP7

印刷

NTA7 mg
NaCl2.5 g
MgSO4 · 7H2O900 mg
KCl70 mg
CaCl2 · 2H2O30 mg
NaNO35 mg
NaH2PO4 · 2H2O2 mg
Vitamin B120.1 μg
Vitamin mix S3 1 )1 mL
Na2SiO3 · 9H2O1 mg
PN metals 2 )3 mL
Tris ( hydroxymethyl ) aminomethane100 mg
Distilled water96 mL
pH 8.0 
1 ) See Vitamin mix S3
2 ) See PN metals
Reference
Watanabe, M. M., Satake, K. N. ( Eds. ) 1991 NIES-Collection. List of Strains, Third Edition, 1991, Microalgae and Protozoa. Microbial Culture Collection, National Institute for Environmental Studies, Tsukuba, 163 pp.k.pdf

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2 海産および汽水産藻類用培地
MF

印刷

f/2 1 ) medium with Na2SiO3 · 9H2O replaced by 1 mL Soil extract 2 ) and adjusted to pH 8.0 by buffering with 100 mg Tris ( hydroxymethyl ) aminomethane.
1 ) See f/2
2 ) See Soil extract

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2 海産および汽水産藻類用培地
mIMR* ( modified IMR )

印刷

KNO31.26 mg
K2HPO40.22 mg
Na2SiO3 1 )0.61 mg
mIMR trace metals 2 )0.1 mL
Thiamine20 μg
Vitamin B121 μg
Biotin0.1 μg
Seawater100 mL
1 ) In the NIES–Collection, Na2SiO3 is replaced by Na2SiO3·9H2O.
2 ) See mIMR trace metals
Reference
Paasche, E., Bruback, S., Skattebol, S., Young, J. R., Green, J. C 1996 Growth and calcification in the coccolithophorid Emiliania huxleyi ( Haptophyceae ) at low salinities. Phycologia, 35, 394-403.

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2 海産および汽水産藻類用培地
MKM

印刷

KNO375 mg
KH2PO42.5 mg
MgSO4 · 7H2O2 mg
Fe–citrate250 μg
Agar1.5 g
Seawater50 mL
Distilled water 50 mL
Reference
Watanabe, A. 1960 List of algal strains in collection at the Institute of Applied Micro-biology, University of Tokyo. J. Gen. Appl. Microbiol., 6, 283-292.

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2 海産および汽水産藻類用培地
MNK*

印刷

NaNO32 mg
K2HPO40.1 mg
Na2HPO4 · 12H2O0.028 mg
Vitamin B120.015 μg
Biotin0.015 μg
Thiamine HCl2 μg
CoSO4 · 7H2O0.12 μg
ZnSO4 · 7H2O0.24 μg
MnCl2 · 4H2O0.9 μg
CuSO4 · 5H2O0.006 μg
Na2SeO30.003 μg
Na2MoO4 · 2H2O0.07 μg
Na2EDTA · 2H2O0.37 μg
Fe–EDTA2.6 μg
Mn–EDTA3.3 μg
Seawater100 mL
Vitamins should be added at the end of the preparation. This medium should not be autoclaved but filter–sterilized.
Reference
Noël, M.-H., Kawachi, M., Inouye, I. 2004 Induced dimorphic life cycle of a coccolithophorid, Calyptrosphaera sphaeroidea ( Prymnesiophyceae, Haptophyta ). J. Phycol., 40, 112-129.

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2 海産および汽水産藻類用培地
PRO–99*

印刷

NaH2PO4 · 2H2O 0.779 mg
NH4Cl4.28 mg
PRO–99 trace metals 1 )0.01 mL
Seawater100 mL
1 ) See PRO–99 trace metals

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2 海産および汽水産藻類用培地
WESM*

印刷

Make diluted seawater by mixing 87.5 mL seawater and 10mL distilled water. Make ESM 1 ) medium by using this diluted seawater instead of seawater.
1 ) See ESM

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3 淡水産藻類用無菌検査培地
B–I

印刷

Appropriate medium100 mL
Proteose peptone100 mg
Reference
Ichimura, T., Watanabe, M. M. 1977 An axenic clone of Microcystis aeruginosa Kutz. emend. Elenkin from Lake Kasumigaura. Bull. Jpn. Soc. Phycol., 25, 177-181.

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3 淡水産藻類用無菌検査培地
B–II

印刷

Appropriate medium100 mL
Yeast extract500 mg
Reference
Ichimura, T., Watanabe, M. M. 1977 An axenic clone of Microcystis aeruginosa Kutz. emend. Elenkin from Lake Kasumigaura. Bull. Jpn. Soc. Phycol., 25, 177-181.

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3 淡水産藻類用無菌検査培地
B–III

印刷

Appropriate medium100 mL
Peptone500 mg
Beef extract300 mg
Reference
Ichimura, T., Watanabe, M. M. 1977 An axenic clone of Microcystis aeruginosa Kutz. emend. Elenkin from Lake Kasumigaura. Bull. Jpn. Soc. Phycol., 25, 177-181.

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3 淡水産藻類用無菌検査培地
B–IV

印刷

Appropriate medium100 mL
Glucose100 mg
Peptone100 mg
Reference
Ichimura, T., Watanabe, M. M. 1977 An axenic clone of Microcystis aeruginosa Kutz. emend. Elenkin from Lake Kasumigaura. Bull. Jpn. Soc. Phycol., 25, 177-181.

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3 淡水産藻類用無菌検査培地
B–V

印刷

Appropriate medium100 mL
Sodium acetate50 mg
Glucose50 mg
Tryptone50 mg
Yeast extract30 mg
Reference
Ichimura, T., Watanabe, M. M. 1977 An axenic clone of Microcystis aeruginosa Kutz. emend. Elenkin from Lake Kasumigaura. Bull. Jpn. Soc. Phycol., 25, 177-181.

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3 淡水産藻類用無菌検査培地
YT

印刷

Appropriate medium100 mL
Yeast extract100 mg
Tryptone200 mg
Reference
Ichimura, T., Itoh, T. 1977 17. Preservation methods of microalgae ( I ) [17. Bisaisôrui no hozonhô ( I ) ]. In Preservation methods of microorganisms [Biseibutsu Hozonhô], Ed. by Nei, T., University of Tokyo Press, Tokyo, p. 355-373 ( in Japanese without English title ).

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4 海産藻類用無菌検査培地
Bf/2

印刷

ASP 7 1 )100 mL
Trypticase50 mg
Yeast extract5 mg
1 ) In the NIES-Collection, ASP 7 is replaced by f/2 medium. See f/2.
Reference
Yoshida, Y., Kawaguchi, K. 1983 Buoyancy and phototaxis of Chattonella antiqua ( Hada ) Ono. Bull. Plankton Soc. Jpn., 30, 11-19 ( in Japanese with English summary ).

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4 海産藻類用無菌検査培地
MM 23 ( M. Tatewaki, pers. comm. )

印刷

NaCl1.8 g
MgSO4 · 7H2O500 mg
KCl60 mg
NaNO3100 mg
CaCl2 · 2H2O36.7 mg
K2HPO46 mg
Sucrose400 mg
PII metals 1 )2 mL
FeCl3 · 6H2O48 μg
Thiamine HCl10 μg
Biotin0.1 μg
Vitamin B120.2 μg
C–Source Mix II 2 )1 mL
Tris ( hydroxymethyl ) aminomethane100 mg
Distilled water97 mL
pH 8.0 
1 ) See PII metals
2 ) See C–Source Mix II

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4 海産藻類用無菌検査培地
STP*

印刷

NaNO320 mg
K2HPO41 mg
Sodium glutamate50 mg
Glucose20 mg
Glycine10 mg
D,L–Alanine10 mg
Vitamin mix 8 1 )0.1 mL
Trypticase20 mg
Yeast autolysate 2 )20 mg
Sucrose100 mg
Soil extract 3 )5 mL
Seawater80 mL
Distilled water15 mL
pH 7.5 

1 ) In the NIES–Collection, Vitamin mix 8 is replaced by

Vitamin mix S3. See Vitamin mix S3.
2 ) In the NIES–Collection, yeast autolysate is replaced by yeast extract.
3 ) See Soil extract

Reference
Provasoli, L., McLaughlin, J. J. A., Droop, M. R. 1957 The development of artificial media for marine algae. Arch. Mikrobiol., 25, 392-428.

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5 微量金属、ビタミン混液、土壌浸出液
A2 Fe stock solution

印刷

EDTA · 2Na700 mg
FeCl3 · 6H2O400 mg
Distilled water100 mL
Sterilize by passing through a Millipore filter ( 0.22 μm ).

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5 微量金属、ビタミン混液、土壌浸出液
A2 trace element stock solution

印刷

H3BO3285 mg
MnCl2 · 4H2O180 mg
ZnCl210.5 mg
Na2MoO4 · 2H2O39 mg
CoCl2 · 6H2O4 mg
CuCl2 · 2H2O4.3 mg
Distilled water100 mL

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5 微量金属、ビタミン混液、土壌浸出液
A5 solution

印刷

H3BO3286 mg
MnSO4 · 7H2O 1 )250 mg
ZnSO4 · 7H2O22.2 mg
CuSO4 · 5H2O7.9 mg
Na2MoO4 · 2H2O2.1 mg
Distilled water100 mL
1 ) In the NIES–Collection, 250 mg MnSO4 · 7H2O is replaced by 217 mg MnSO4 · 5H2O.
Reference
Holm-Hansen, O., Gerloff, G. C., Skoog, F. 1954 Cobalt as an essential element for blue-green algae. Physiol. Planta., 7, 665-675.

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5 微量金属、ビタミン混液、土壌浸出液
Allen metals

印刷

Fe–EDTA30.16 g
MnCl2 · 4H2O1.79 g
H3BO32.86 g
ZnSO4 · 7H2O220 mg
CuSO4 · 5H2O79 mg
( NH4 )6Mo7O24 · 4H2O130 mg
NH4VO323 mg
Distilled water100 mL
In the NIES–Collection, Allen metals are used after dilution with distilled water to 1/1,000.
Reference
Allen, M. B. 1959 Studies with Cyanidium caldarium, an anomalously pigmented chlorophyte. Arch. Mikrobiol., 32, 270-277.

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5 微量金属、ビタミン混液、土壌浸出液
C–Source Mix II ( M. Tatewaki, pers. comm. )

印刷

Glycine100 mg
D,L-Alanine100 mg
L-Asparagine100 mg
Sodium acetate · 3H2O 1 )200 mg
Glucose200 mg
L-Glutamic acid200 mg
Distilled water100 mL
1 ) In the NIES–Collection, 200 mg sodium acetate · 3H2O is replaced by 120 mg sodium acetate, anhydrous.

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5 微量金属、ビタミン混液、土壌浸出液
D stock medium

印刷

NTA0.2 g
D trace mix 1 )1 mL
FeCl3 · 6H2O0.58 mg
CaSO4 · 2H2O120 mg
MgSO4 · 7H2O200 mg
NaCl16 mg
KNO3200 mg
NaNO31.4 g
Na2HPO4 2 )220 mg
Distilled water99 mL
1 ) See D trace mix
2 ) In the NIES-Collection, 220 mg Na2HPO4 is replaced by 550 mg Na2HPO4· 12H2O.
Reference
Castenholz, R. W. 1969 Thermophilic blue-green algae and the thermal environment. Bacteriol. Rev., 33, 476-504.

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5 微量金属、ビタミン混液、土壌浸出液
D trace mix

印刷

conc · H2SO40.05 mL
MnSO4 · H2O 1 )228 mg
ZnSO4 · 7H2O50 mg
H3BO350 mg
CuSO4 · 5H2O2.5 mg
Na2MoO4 · 2H2O2.5 mg
CoCl2 · 6H2O4.5 mg
Distilled water100 mL
1 ) In the NIES–Collection, 228 mg MnSO4 · H2O is replaced by 349 mg MnSO4 · 5H2O.
Reference
Castenholz, R. W. 1969 Thermophilic blue-green algae and the thermal environment. Bacteriol. Rev., 33, 476-504.

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5 微量金属、ビタミン混液、土壌浸出液
DY trace metal solution

印刷

MnCl2 · 4H2O20 mg
ZnSO4 · 7H2O4 mg
CoCl2 · 6H2O0.8 mg
Na2MoO4 · 6H2O 1 )2 mg
Na3VO4 · 10H2O 2 )0.2 mg
H2SeO30.2 mg
Distilled water100 mL
1 ) In the NIES–Collection, Na2MoO4 · 6H2O is replaced by Na2MoO4 · 2H2O.
2 ) In the NIES-Collection, 0.2 mg Na3VO4 · 10H2O is replaced by 0.1 mg Na3VO4.

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5 微量金属、ビタミン混液、土壌浸出液
Fe ( as EDTA; 1:1 molar )*

印刷

Fe( NH4 )2( SO4 )2 · 6H2O70.2 mg
Na2EDTA · 2H2O66 mg
Distilled water100 mL
1 mL of   this solution contains 0.1 mg Fe.
Reference
Provasoli, L. 1966 Media and prospects for the cultivation of marine algae. In Cultures and Collections of Algae, Eds. by Watanabe, A. & Hattori, A., Proc. U.S.-Japan Conf., Hakone, Sept. 1966., Jpn. Soc. Plant Physiol., p. 63-75.

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5 微量金属、ビタミン混液、土壌浸出液
Fe solution

印刷

FeSO4 · 7H2O200 mg
Distilled water100 mL
Conc · H2SO4 0.026 mL 1 )
1 ) 2 drops/500 mL
Reference
Ichimura, T., Itoh, T. 1977 17. Preservation methods of microalgae ( I ) [17. Bisaisôrui no hozonhô ( I )]. In Preservation methods of microorganisms [Biseibutsu Hozonhô], Ed. by Nei, T., University of Tokyo Press, Tokyo, p. 355-373 ( in Japanese without English title ).

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5 微量金属、ビタミン混液、土壌浸出液
f/2 metals*

印刷

Na2EDTA · 2H2O440 mg
FeCl3 · 6H2O316 mg
CoSO4 · 7H2O1.2 mg
ZnSO4 · 7H2O2.1 mg
MnCl2 · 4H2O18 mg
CuSO4 · 5H2O0.7 mg
Na2MoO4 · 2H2O0.7 mg
Distilled water100 mL
Reference
Guillard, R. R. L., Ryther, J. H. 1962 Studies of marine planktonic diatoms. I. Cyclotella nana Hustedt, and Detonula confervacea ( Cleve ) Gran. Can. J. Microbiol., 8, 229-239.

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5 微量金属、ビタミン混液、土壌浸出液
K metals

印刷

Na2EDTA · 2H2O3.722 g
Fe-EDTA0.493 g
FeCl3 · 6H2O0.315 g
MnCl2 · 4H2O17.8 mg
ZnSO4 · 7H2O2.3 mg
CoSO4 · 7H2O1.405 mg
Na2MoO4 · 2H2O0.726 mg
CuSO4 · 5H2O0.25 mg
Distilled water100 mL
Reference
Keller, M. D., Guillard, R.R.L. 1985 Factors significant to marine diatom culture. pp. 113-6. In Anderson, D.M., White, A.W., Baden, D.G. (eds.) Toxic Dinoflagellates. Elsevier, New York.
Keller, M.D., Selvin, R.C., Claus, W., Guillard, R.R.L. 1987. Media for the culture of oceanic ultraphytoplankton. J. Phycol., 23: 633-638.

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5 微量金属、ビタミン混液、土壌浸出液
K/2 metals

印刷

Na2EDTA · 2H2O3.722 g
CuSO4 · 5H2O0.25 mg
Na2MoO4 · 2H2O0.726 mg
ZnSO4 · 7H2O2.3 mg
CoSO4 · 7H2O1.405 mg
MnCl2 · 4H2O17.81 mg
H2SeO30.129 mg
NiCl2 · 6H2O 1) 0.149 mg
Distilled water100 mL
1 ) In the NIES–Collection, 0.149 mg NiCl2 · 6H2O is replaced by 0.812 mg NiCl2.
Reference
Guillard, R. R. L., Ryther, J. H. 1962 Studies of marine planktonic diatoms. I. Cyclotella nana Hustedt, and Detonula confervacea ( Cleve ) Gran. Can. J. Microbiol., 8, 229-239.

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5 微量金属、ビタミン混液、土壌浸出液
Hutner ’s trace elements

印刷

Na2EDTA · 2H2O5 g
ZnSO4 · 7H2O2.2 g
H3BO31.14 g
MnCl2 · 4H2O506 mg
FeSO4 · 7H2O499 mg
CoCl2 · 6H2O161 mg
CuSO4· 5H2O157 mg
( NH4 )6Mo7O24 · 4H2O110 mg
Distilled water100 mL
Adjust the pH to 6.5-6.8 with KOH ( ~1.6 g ). Store the solution in a refrigerator ( 5°C ). The solution should have turned to violet color before use. This process takes a while and is necessary.

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5 微量金属、ビタミン混液、土壌浸出液
mIMR trace metals*

印刷

FeCl3 · 6H2O100 mg
MnSO4 · 4H2O85.5 mg
ZnSO4 · 7H2O25 mg
Na2MoO4 · 2H2O13 mg
CoCl2 · 6H2O0.4 mg
CuSO4 · 5H2O0.4 mg
Na2SeO30.173 mg
Na2EDTA 1 )600 mg
Distilled water100 mL
1 ) In the NIES–Collection, Na2EDTA is replaced by Na2EDTA · 2H2O.

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5 微量金属、ビタミン混液、土壌浸出液
mM–1 Trace elements

印刷

CuSO4 · 5H2O10 mg
MnCl2 · 4H2O 10 mg
Br ( 1mol / L solution )0.01 mL
ZnSO4 · 7H2O 10 mg
CoCl2 · 6H2O5 mg
BaCl2 · 2H2O1 mg
H3BO310 mg
FeCl3 · 6H2O 10 mg
Na2MoO4 · 2H2O5 mg
Distilled water100 mL
Indicated as “Trace elements” in reference.
Reference
Hoham, R. W., Berman, J. D., Rogers, H. S., Felio, J. H., Ryba, J. B., Miller, P. R. 2006 Two new species of green snow algae from Upstate New York, Chloromonas chenangoensis sp. nov. and Chloromonas tughillensis sp. nov. ( Volvocales, Chlorophyceae ) and the effects of light on their life cycle development. Phycologia, 45, 319-330.

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5 微量金属、ビタミン混液、土壌浸出液
mTYGM–9*

印刷

Pre–solution
K2HPO40.28 g
KH2PO440 mg
Casein Digest0.2 g
Yeast extract0.1 g
NaCl0.75 g
Mucin, gastric0.2 g
Distilled water97 mL
Sterilize Pre–solution by autoclaving ( 121°C, 15 min ) , add aseptically 3 mL horse serum and 50 μL 10% Tween 80 dissolved in absolute ethanol ( both filter–sterilized ). Keep in a cool place.

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5 微量金属、ビタミン混液、土壌浸出液
P II metals

印刷

Na2EDTA · 2H2O100 mg
H3BO3114 mg
FeCl3 · 6H2O4.9 mg
MnSO4 · 4H2O16.4 mg
ZnSO4 · 7H2O2.2 mg
CoSO4 · 7H2O480 μg
Distilled water100 mL
Reference
Provasoli, L. 1963 Growing marine seaweeds. In Proceedings of the Fourth International Seaweed Symposium, University of Tokyo Press, Tokyo, p. 9-17.

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5 微量金属、ビタミン混液、土壌浸出液
P IV metals*

印刷

Na2EDTA · 2H2O100 mg
FeCl3 · 6H2O19.6 mg
MnCl2 · 4H2O3.6 mg
ZnCl2 1 )1.04 mg
CoCl2 · 6H2O0.4 mg
Na2MoO4 · 2H2O0.25 mg
Distilled water100 mL
1 ) In the NIES–Collection, 1.04mg ZnCl2 is replaced by 2.2mg ZnSO4 · 7H2O.
Reference
Provasoli, L., Pintner, I. J. 1959 Artificial media for fresh-water algae: problems and suggestions. In The Ecology of Algae. Spec. Pub. No. 2,, Eds. by Tryon, C. A., Jr. & Hartmann, R. T., Pymatuning Laboratory of Field Biology, University of Pittsburgh, Pittsburgh, p. 84-96.

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5 微量金属、ビタミン混液、土壌浸出液
PN metals

印刷

Na2EDTA · 2H2O100 mg
H3BO3113 mg
FeCl3 · 6H2O6.3 mg
CoSO4 · 7H2O0.093 mg
ZnSO4 · 7H2O4.66 mg
MnCl2 · 4H2O3.2 mg
Distilled water100 mL
Reference
Watanabe, M. M., Satake, K. N. ( Eds. ) 1991 NIES-Collection. List of Strains, Third Edition, 1991, Microalgae and Protozoa. Microbial Culture Collection, National Institute for Environmental Studies, Tsukuba, 163 pp.

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5 微量金属、ビタミン混液、土壌浸出液
PRO–99 trace metals

印刷

Na2EDTA 1 )0.58 g
FeCl3 · 6H2O0.422 g
ZnSO4 · 7H2O2.933 mg
CoCl2 · 6H2O1.332 mg
MnCl2 · 4H2O24 mg
Na2MoO4 · 2H2O0.839 g
Na2SeO32.306 mg
NiCl2 · 6H2O0.37 mg
Distilled water100 mL
1 ) In the NIES–Collection, 0.58 g Na2EDTA is replaced by 0.637 g Na2EDTA · 2H2O.

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5 微量金属、ビタミン混液、土壌浸出液
Soil extract

印刷

To 1000 mL distilled water add 200 mL of soil ( soil from undisturbed deciduous woodland is best ) and heat by autoclaving for 1 h at 105°C. When cool, heat by autoclaving for 1 h at 105°C again. Pass the supernatant through a GF/C filter and Celite, and then pass the filtrate through a GF/F filter. Adjust to 1000 mL by adding distilled water. Dispense 10 mL of the final filtrate into each test tube and sterilize by autoclaving for 20min at 121°C. Keep in a cool place.
Reference
Provasoli, L., McLaughlin, J. J. A., Droop, M. R. 1957 The development of artificial media for marine algae. Arch. Mikrobiol., 25, 392-428.

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5 微量金属、ビタミン混液、土壌浸出液
Trace metal mix A5 + Co

印刷

H3BO3286 mg
MnCl2 · 4H2O181 mg
ZnSO4 · 7H2O22.2 mg
Na2MoO4 · 2H2O39 mg
CuSO4 · 5H2O7.9 mg
Co( NO3 )2·6H2O4.9 mg
Distilled water100 mL

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5 微量金属、ビタミン混液、土壌浸出液
Vitamin mix S3

印刷

Thiamine HCl5 mg
Nicotinic acid1 mg
Calcium pantothenate1 mg
p–Aminobenzoic acid0.1 mg
Biotin0.01 mg
Inositol50 mg
Folic acid0.02 mg
Thymine30 mg
Distilled water100 mL
Reference
Provasoli, L. 1963 Growing marine seaweeds. In Proceedings of the Fourth International Seaweed Symposium, University of Tokyo Press, Tokyo, p. 9-17.

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5 微量金属、ビタミン混液、土壌浸出液
Waris EDTA

印刷

EDTA0.522 g
FeSO4 · 7H2O0.498 g
KOH (1 mol/L solution)5.4 mL
Distilled water94.6 mL
EDTA and FeSO4 · 7H2O is heated for 30 min (100°C ); KOH is added to the cooled mixture.
Reference
McFadden, G. I., Melkonian, M. 1986 Use of Hepes buffer for microalgael culture media and fixation for eledtron microscopy. Phycology, 25, 551-557.

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5 微量金属、ビタミン混液、土壌浸出液
Waris P-2

印刷

Na2EDTA· 2H2O0.3 g
H3BO30.114 g
MnCl2· 4H2O14.4 mg
ZnSO4·7H2O2.1 mg
CoCl2·6H2O 0.4 mg
Distilled water100 mL
Reference
McFadden, G. I., Melkonian, M. 1986 Use of Hepes buffer for microalgael culture media and fixation for eledtron microscopy. Phycology, 25, 551-557.

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6.原生動物用培地
AF–6 + Wheat

印刷

Beforehand, sterilize wheat grains by dry heating ( 150ºC, 30 min ). Keep in a cool place. For use, add a grain of sterile wheat to 10 mL AF–6 1 ) medium.
1 ) See AF–6

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6.原生動物用培地
ESM + mTYGM–9 + Rice

印刷

Beforehand, sterilize polished rice by dry heating ( 150ºC, 30 min ). Keep in a cool place. For use, add 500 μL mTYGM–9 1 ) and a grain of sterile rice to 10 mL ESM 2 ) medium.
1 ) See mTYGM–9
2 ) See ESM

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6.原生動物用培地
f/2 + mTYGM–9 + Rice

印刷

Beforehand, sterilize polished rice by dry heating ( 150ºC, 30 min ). Keep in a cool place. For use, add 500 μL mTYGM–9 1 ) and a grain of sterile rice to 10 mL f/2 2 ) medium.
1 ) See mTYGM–9
2 ) See f/2

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6.原生動物用培地
f/2 + Wheat

印刷

Beforehand, sterilize wheat grains by dry heating ( 150ºC, 30 min ). Keep in a cool place. For use, add a grain of sterile wheat to 10 mL f/2 1 ) medium.
1 ) See f/2

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6.原生動物用培地
Helio*

印刷

Sodium acetate10 mg
Polypeptone10 mg
Tryptone20 mg
Yeast extract20 mg
CaCl2 · 2H2O0.1 mg
Sea water10 mL
Distilled water90 mL

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6.原生動物用培地
LE

印刷

L solution: White part of lettuce is dried at 90ºC for 16–18 h without scorching; 300 mg of the dried lettuce is added to 100 mL boiling water ( 9:1 distilled water to tap water ) and boiled for 30 min, while stirring. The supernatant is passed through cotton wool.
E solution: 300 mg of crushed yolk of hardboiled egg is added to 100 mL water ( 9:1 distilled water to tap water ) and boiled for 30 min, while stirring. The supernatant is passed through cotton wool.
Equal quantities of L and E solutions are mixed. The pH is adjusted to 6.8–7.0 with 1 mol / L NaOH, and 100 mL of the solution is dispensed into each 200–mL Erlenmeyer flask and sterilized by autoclaving ( 121ºC, 15 min ).

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6.原生動物用培地
PYG*

印刷

Proteose Peptone0.15 g
Yeast extract0.1 g
Glucose0.5 g
Distilled water100 mL
pH 7.0 
PPYG medium is diluted half strength and reduced proteose peptone.
Reference
Mori, K., Yomo, T., Kashiwagi, A. 2011 Single-cell isolation and cloning of tetrahymena thermophila cells with a fluorescence-activated cell sorter. J. Eukaryotic Microbiol., 58(1), 37-42.

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6.原生動物用培地
SUY*

印刷

Prepare as for 100 mL URO 1 ) medium with seawater instead of distilled water. Add 10 mg yeast extract and 20 mg tryptone. Indicated as " URO–YT " in reference.
1 ) See URO
Reference
Moriya, M., Nakayama, T., Inouye, I. 2000 Ultrastructure and 18S rDNA sequence analysis of Wobblia lunata gen. et sp. nov., a new heterotrophic flagellate ( Stramenopiles, incertae sedis ). Protist, 151, 41-55.

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6.原生動物用培地
SUY 1/10*

印刷

Prepare as for 100 mL URO 1 ) medium with seawater instead of distilled water. Add 1 mg yeast extract and 2 mg tryptone. Indicated as " URO–1/10 YT " in reference.
1 ) See URO
Reference
Moriya, M., Nakayama, T., Inouye, I. 2002 A new class of the Stramenopiles, Placididea Classis nova: description of Placidia cafeteriopsis gen. et sp. nov. Protist, 153, 143-156.

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6.原生動物用培地
SUY 1/10 + mTYGM–9 + Rice

印刷

Beforehand, sterilize polished rice by dry heating ( 150ºC, 30 min ). Keep in a cool place. For use, add 500 μL mTYGM–9 1 ) and a grain of sterile rice to 10 mL SUY 1/10 2 ) medium.
1 ) See mTYGM–9
2 ) See SUY 1/10

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6.原生動物用培地
SUY 1/10 + Wheat

印刷

Beforehand, sterilize wheat grains by dry heating ( 150ºC, 30 min ). Keep in a cool place. For use, add a grain of sterile wheat to 10 mL SUY 1/10 1 ) medium.
1 ) See SUY 1/10

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6.原生動物用培地
URO + Wheat

印刷

Beforehand, sterilize wheat grains by dry heating ( 150ºC, 30 min ). Keep in a cool place. For use, add a grain of sterile wheat to 10 mL URO 1 ) medium.
1 ) See URO

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6.原生動物用培地
URO–H + Wheat

印刷

Beforehand, sterilize wheat grains by dry heating ( 150ºC, 30 min ). Keep in a cool place. For use, add a grain of sterile wheat to 10 mL URO–H 1 ) medium.
1 ) See URO–H

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6.原生動物用培地
UYTS + Rice

印刷

Prepare as for 100 mL URO 1 ) medium with 99.2 mL instead of 99.9 mL distilled water. Adjust to pH 7.5 with 0.1 mol / L HCl, and add 10 mg yeast extract, 20 mg tryptone and 0.3 ml horse serum ( UYTS medium ). Beforehand, sterilize polished rice by dry heating ( 150ºC, 30 min ). Keep in a cool place. For use, add a grain of sterile rice to 10 mL UYTS medium.
1 ) See URO

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6.原生動物用培地
1/2Waris H+Si*

印刷

HEPES11.915 mg
KNO35 mg
MgSO4 · 7H2O1 mg
(NH4)2HPO41 mg
Ca(NO3)2 · 4H2O5 mg
Waris P-20.05 mL
Waris EDTA0.05 mL
Vitamin B121 μg
Biotin0.05 μg
Thiamine HCl5 μg
Niacinamide0.005 μg
Soil estract 3 )0.5 mL
Na2SiO3 · 9H2O2.842 mg
Distilled water99.4 mL
pH 7.0 
Waris H is diluted half strength and added Na2SiO3 · 9H2O.
1 ) See Waris P-2
2 ) See Waris EDTA
3 ) See Soil extract
Reference
McFadden, G. I., Melkonian, M. 1986 Use of Hepes buffer for microalgael culture media and fixation for eledtron microscopy. Phycology, 25, 551-557.

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7.淡水産紅藻用培地
Bold 3N*

印刷

NaNO375 mg
CaCl2 · 2H2O2.5 mg
MgSO4 · 7H2O7.5 mg
K2HPO47.5 mg
KH2PO4 1 )17.5 mg
NaCl2.5 mg
Vitamin B12 2 )0. 015 μg
PIV metals 3 )0.6 mL
Soil extract 4 )4 mL
Distilled water96.4 mL
1 ) In the NIES–Collection, the amount of KH2PO4 is reduced from 17.5 mg to 10.5 mg.
2 ) In the NIES–Collection, the amount of Vitamin B12 is increased from 0.015 μg to 0.02 μg.
3 ) See PIV metals
4 ) See Soil extract

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8.シャジクモ類用培地
mSWC–2 ( Modified SWC–2 )

印刷

Put leaf mould into a glass vessel to make a thin bottom layer, and add river sand onto the bottom layer up to one–quarter to one–fifth from the bottom. Add a pinch of garden lime to the river sand before use. Dampen the soil with deionized water ( or distilled water ). Cover the glass vessel with a plastic cap or aluminum foil, and autoclave it twice with overnight rest in between ( 121ºC, 20 min ). After cooling the mixture to room temperature, pour sterilized deionized water ( or sterilized distilled water ) into it carefully ( so as not to disturb the soil ). In the case of brackish water strains, deionized water is replaced by about one–third–diluted Herbst artificial seawater ( 1/3 Herbst ASW ).

Reference
Sakayama, H., Hara Y., Nozaki, H. 2004 Taxonomic re-examination of six species of Nitella ( Charales, Charophyceae ) from Asia, and phylogenetic relationships within the genus based on rbcL and atpB gene sequences. Phycologia, 43, 91-104.

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8.シャジクモ類用培地
SWC–1

印刷

Put leaf mould into a glass vessel to make a thin bottom layer, and add black soil onto the bottom layer up to one-quarter to one–fifth from the bottom. Dampen the soil with deionized water ( or distilled water ). Cover the glass vessel with a plastic cap or aluminum foil, and autoclave it twice with overnight rest in between ( 121ºC, 20 min ). After cooling the mixture to room temperature, pour sterilized deionized water ( or sterilized distilled water ) into it carefully ( so as not to disturb the soil ).
Reference
Sakayama, H., Hara Y., Nozaki, H. 2004 Taxonomic re-examination of six species of Nitella ( Charales, Charophyceae ) from Asia, and phylogenetic relationships within the genus based on rbcL and atpB gene sequences. Phycologia, 43, 91-104.

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8.シャジクモ類用培地
SWCN–1

印刷

Put bottom mud from a paddy field into a glass vessel up to one–quarter to one–fifth from the bottom. Dampen the mud with deionized water ( or distilled water ). Cover the glass vessel or jar with a plastic cap or aluminum foil, and autoclave it twice with overnight rest in between ( 121ºC, 20 min ). After cooling the mixture to room temperature, pour sterilized deionized water ( or sterilized distilled water ) into it carefully ( so as not to disturb the soil ).

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8.シャジクモ類用培地
SWCN–2

印刷

Put leaf mould into a glass vessel to make a thin bottom layer, and add a mixture of black soil and river sand onto the bottom layer up to one–quarter to one–fifth from the bottom. Dampen the soil with deionized water ( or distilled water ). Cover the glass vessel or jar with a plastic cap or aluminum foil, and autoclave it twice with overnight rest in between ( 121ºC, 20 min ). After cooling the mixture to room temperature, pour sterilized deionized water ( or sterilized distilled water ) into it carefully ( so as not to disturb the soil ). In the case of brackish water strains, deionized water is replaced by about one–third–diluted Herbst artificial seawater ( 1/3 Herbst ASW ).

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8.シャジクモ類用培地
SWCN–3

印刷

Put a mixture of black soil and bottom mud from a paddy field into a glass vessel up to one–quarter to one–fifth from the bottom. Dampen the soil with deionized water ( or distilled water ). Cover the glass vessel or jar with a plastic cap or aluminum foil, and autoclave it twice with overnight rest in between ( 121ºC, 20 min ). After cooling the mixture to room temperature, pour sterilized deionized water ( or sterilized distilled water ) into it carefully ( so as not to disturb the soil ).

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8.シャジクモ類用培地
SWCN–4

印刷

Put a mixture of river sand and bottom mud from a paddy field into a glass vessel up to one–quarter to one–fifth from the bottom. Dampen the soil with deionized water ( or distilled water ). Cover the glass vessel or jar with a plastic cap or aluminum foil, and autoclave it twice with overnight rest in between ( 121ºC, 20 min ). After cooling the mixture to room temperature, pour sterilized deionized water ( or sterilized distilled water ) into it carefully ( so as not to disturb the soil ).

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8.シャジクモ類用培地
1/3 Herbst ASW*

印刷

NaCl3.0 mg
KCl 1 )81.4 mg
CaCl2 1 )132 mg
MgSO4 1 )660 mg
NaHCO3 1 )504 mg
Distilled water100 mL

1 ) In the NIES–Collection, the amount of KCl is reduced from 81.4 mg to 80.0 mg, 132 mg CaCl2 is replaced by 172 mg CaCl2 · 2H2O, 660 mg MgSO4 is replaced by 1.35 g MgSO4 · 7H2O, and the amount of NaHCO3 is reduced from 504 mg to 49.5 mg.
Reference
Okazaki, Y., Shimmen, T., Tazawa, M. 1984 Turgor regulation in a brackish charophyte, Lamprothamnium succinctum I. Artificial modification of intracellular osmotic pressure. Plant Cell Physiol., 25, 565-571.

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